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Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

  • Cat.No.:E-EL-R1327

  • Reactivity: Rat

To Purchase E-EL-R1327

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat CKMB in samples. No significant cross-reactivity or interference between Rat CKMB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat CKMB concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CKMB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CKMB were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 93.35 286.64 760.84 100.16 266.78 728.82
Standard deviation 4.85 13.27 36.44 6.24 15.71 23.91
CV (%) 5.20 4.63 4.79 6.23 5.89 3.28


The recovery of Rat CKMB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 93
EDTA plasma (n=8) 90-105 98
Cell culture media (n=8) 87-99 93


Samples were spiked with high concentrations of Rat CKMB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCardiovascular, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Circulation (2017) IF: 19.309
    Functional Metabolomics Characterizes A Key Role For N-Acetyl-Neuraminic Acid In Coronary Artery Diseases.

    DOI: 10.1161/CIRCULATIONAHA.117.031139

    PMID: 29212895

    Sample: Cell culture supernatant
  2. Nature Communications (2021) IF: 14.919
    The long noncoding RNA lncCIRBIL disrupts the nuclear translocation of Bclaf1 alleviating cardiac ischemia–reperfusion injury

    DOI: 10.1038/s41467-020-20844-3

    PMID: 33483496

    Sample: Plasma
  3. Cardiovascular Diabetology (2016) IF: 4.752
    N-Acetylcysteine Attenuates Myocardial Dysfunction And Postischemic Injury By Restoring Caveolin-3/eNOS Signaling In Diabetic Rats.

    DOI: 10.1186/s12933-016-0460-z

    PMID: 27733157

    Sample: Serum
  4. Clinical Science (2016) IF: 4.936
    Selective Inhibition Of PTEN Preserves Ischemic Postconditioning Cardioprotection In STZ-Induced Type1 Diabetic Rats: Role Of The PI3K/ Akt And JAK2/ STAT3 Pathways.

    DOI: 10.1042/CS20150496

    PMID: 26666444

    Sample: Serum
  5. European review for medical and pharmacological sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (miR-2) and miR-101 on sepsis-induced myocardial injury in rats

    DOI: 10.26355/eurrev_202006_21528

    Sample: Serum
  6. Immunological Investigations (2020) IF: 2.511
    Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase-1/IL-1β Signaling Pathway Inhibition

    DOI: 10.1080/08820139.2020.1791178

    PMID: 32674625

    Sample: Cell culture supernatant
  7. Molecular Medicine Reports (2017) IF: 2.1
    AMPK activation restores ischemic post-conditioning cardioprotection in STZ-induced type 1 diabetic rats: Role of autophagy

    DOI: 10.3892/mmr.2017.7033

    PMID: 28765969

    Sample: Serum
  8. RSC Advances (2019) IF: 2.936
    Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation

    DOI: 10.1039/C8RA08662A

    Sample: Tissue homogenate
  9. Journal of Pharmacy and Pharmacology (2018) IF: 2.309
    Total flavones of Rhododendron simsii Planch flower protect isolated rat heart from ischaemia-reperfusion injury and its mechanism of UTR-RhoA-ROCK pathway inhibition

    DOI: 10.1111/jphp.13016

    PMID: 30251312

    Sample: Tissue homogenate
  10. Journal of biochemical and molecular toxicology (2019) IF: 1.837
    Hesperidin Regresses Cardiac Hypertrophy by Virtue of PPAR-γ Agonistic, Anti-Inflammatory, Antiapoptotic, and Antioxidant Properties

    DOI: 10.1002/jbt.22283

    PMID: 30623541

    Sample: Serum
  11. Molecular Medicine Reports (2015) IF: 1.692
    Candesartan Ameliorates Acute Myocardial Infarction In Rats Through Inducible Nitric Oxide Synthase, Nuclear FactorκB, Monocyte Chemoattractant Protein1, Activator Protein1 And Restoration Of Heat Shock Protein 72.

    DOI: 10.3892/mmr.2015.4432

    PMID: 26499133

    Sample: Serum
  12. International Journal of Clinical and Experimental Pathology (2016) IF: 1.706
    Increased Expression Of RP105 Decreases Cardiomyocytes Hypoxia/Reoxygenation Injury Through The TLR4/NF-κB Signaling Pathway.
    Sample: Cell culture supernatant
  13. Xenobiotica (2015) IF: 1.932
    Protective Effects Of Berberine Against Doxorubicin-Induced Cardiotoxicity In Rats By Inhibiting Metabolism Of Doxorubicin.

    DOI: 10.3109/00498254.2015.1034223

    PMID: 25970816

    Sample: Serum
  14. Journal of Drug Delivery and Therapeutics (2020)
    Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats

    DOI: 10.22270/jddt.v10i1-s.3868

    Sample: Serum
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