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Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit

Cat.No.:E-EL-R1327
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat CKMB in samples. No significant cross-reactivity or interference between Rat CKMB and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat CKMB concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CKMB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CKMB were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	93.35	286.64	760.84	100.16	266.78	728.82
Standard deviation	4.85	13.27	36.44	6.24	15.71	23.91
CV (%)	5.20	4.63	4.79	6.23	5.89	3.28

Recovery

The recovery of Rat CKMB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	93
EDTA plasma (n=8)	90-105	98
Cell culture media (n=8)	87-99	93

Linearity

Samples were spiked with high concentrations of Rat CKMB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CKMB
Research Area	Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Circulation (2017) IF: 19.309

Functional Metabolomics Characterizes A Key Role For N-Acetyl-Neuraminic Acid In Coronary Artery Diseases.

DOI: 10.1161/CIRCULATIONAHA.117.031139
PMID: 29212895	Sample: Cell culture supernatant

Nature Communications (2021) IF: 14.919

The long noncoding RNA lncCIRBIL disrupts the nuclear translocation of Bclaf1 alleviating cardiac ischemia–reperfusion injury

DOI: 10.1038/s41467-020-20844-3
PMID: 33483496	Sample: Plasma

Cardiovascular Diabetology (2016) IF: 4.752

N-Acetylcysteine Attenuates Myocardial Dysfunction And Postischemic Injury By Restoring Caveolin-3/eNOS Signaling In Diabetic Rats.

DOI: 10.1186/s12933-016-0460-z
PMID: 27733157	Sample: Serum

Clinical Science (2016) IF: 4.936

Selective Inhibition Of PTEN Preserves Ischemic Postconditioning Cardioprotection In STZ-Induced Type1 Diabetic Rats: Role Of The PI3K/ Akt And JAK2/ STAT3 Pathways.

DOI: 10.1042/CS20150496
PMID: 26666444	Sample: Serum

European review for medical and pharmacological sciences (2020) IF: 3.024

The effect of myocardial infarction-associated transcript 2 (miR-2) and miR-101 on sepsis-induced myocardial injury in rats

DOI: 10.26355/eurrev_202006_21528
	Sample: Serum

Immunological Investigations (2020) IF: 2.511

Overexpression of lncRNA HULC Attenuates Myocardial Ischemia/reperfusion Injury in Rat Models and Apoptosis of Hypoxia/reoxygenation Cardiomyocytes via Targeting miR-377-5p through NLRP3/Caspase-1/IL-1β Signaling Pathway Inhibition

DOI: 10.1080/08820139.2020.1791178
PMID: 32674625	Sample: Cell culture supernatant

Molecular Medicine Reports (2017) IF: 2.1

AMPK activation restores ischemic post-conditioning cardioprotection in STZ-induced type 1 diabetic rats: Role of autophagy

DOI: 10.3892/mmr.2017.7033
PMID: 28765969	Sample: Serum

RSC Advances (2019) IF: 2.936

Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation

DOI: 10.1039/C8RA08662A
	Sample: Tissue homogenate

Journal of Pharmacy and Pharmacology (2018) IF: 2.309

Total flavones of Rhododendron simsii Planch flower protect isolated rat heart from ischaemia-reperfusion injury and its mechanism of UTR-RhoA-ROCK pathway inhibition

DOI: 10.1111/jphp.13016
PMID: 30251312	Sample: Tissue homogenate

Journal of biochemical and molecular toxicology (2019) IF: 1.837

Hesperidin Regresses Cardiac Hypertrophy by Virtue of PPAR-γ Agonistic, Anti-Inflammatory, Antiapoptotic, and Antioxidant Properties

DOI: 10.1002/jbt.22283
PMID: 30623541	Sample: Serum

Molecular Medicine Reports (2015) IF: 1.692

Candesartan Ameliorates Acute Myocardial Infarction In Rats Through Inducible Nitric Oxide Synthase, Nuclear FactorκB, Monocyte Chemoattractant Protein1, Activator Protein1 And Restoration Of Heat Shock Protein 72.

DOI: 10.3892/mmr.2015.4432
PMID: 26499133	Sample: Serum

International Journal of Clinical and Experimental Pathology (2016) IF: 1.706

Increased Expression Of RP105 Decreases Cardiomyocytes Hypoxia/Reoxygenation Injury Through The TLR4/NF-κB Signaling Pathway.


	Sample: Cell culture supernatant

Xenobiotica (2015) IF: 1.932

Protective Effects Of Berberine Against Doxorubicin-Induced Cardiotoxicity In Rats By Inhibiting Metabolism Of Doxorubicin.

DOI: 10.3109/00498254.2015.1034223
PMID: 25970816	Sample: Serum

Journal of Drug Delivery and Therapeutics (2020)

Nigella sativa seed extract protects against cadmium-induced cardiotoxicity in rats

DOI: 10.22270/jddt.v10i1-s.3868
	Sample: Serum

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