Current Position:Home>>Kits>>ELISA Kits>> Rat COL1(Collagen Type Ⅰ) ELISA Kit

Rat COL1(Collagen Type Ⅰ) ELISA Kit

Cat.No.:E-EL-R3008
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R3008

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	7.81-500 ng/mL
Sensitivity	4.69 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat COL1 in samples. No significant cross-reactivity or interference between Rat COL1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat COL1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL1. You can calculate the concentration of Rat COL1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat COL1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat COL1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	20.63	55.58	244.42	23.19	56.65	218.01
Standard deviation	1.05	2.56	12.27	1.16	2.83	8.72
CV (%)	5.09	4.61	5.02	5.00	5.00	4.00

Recovery

The recovery of Rat COL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-100	97
EDTA plasma (n=8)	95-103	99
Cell culture media (n=8)	88-94	91

Linearity

Samples were spiked with high concentrations of Rat COL1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 7.446

Design, Synthesis, and Biological Evaluation of Novel Evodiamine Derivatives as Potential Antihepatocellular Carcinoma Agents

DOI: 10.1021/acs.jmedchem.2c00520
	Sample: HSC-T6 cell

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2018) IF: 6.514

Design, synthesis and biological evaluation of non-secosteriodal vitamin D receptor ligand bearing double side chain for the treatment of chronic pancreatitis

DOI: 10.1016/j.ejmech.2018.01.073
PMID: 29407979	Sample: Cell culture supernatant

PHYTOMEDICINE (2021) IF: 5.34

Effective-components combination improves airway remodeling in COPD rats by suppressing M2 macrophage polarization via the inhibition of mTORC2 activity

DOI: 10.1016/j.phymed.2021.153759
PMID: 34600177	Sample: bronchoalveolar lavage fluid (BALF)

Journal of Materials Chemistry B (2018) IF: 4.776

Comparative evaluation of the biocompatible and physical–chemical properties of poly(lactide-co-glycolide) and polydopamine as coating materials for bacterial cellulose

DOI: 10.1039/C8TB02456A
	Sample: fibroblast cells (RFL-6 cells)

European Review for Medical and Pharmacological Sciences (2020) IF: 3.024

Effects of Secreted frizzled-related protein 1 on inhibiting cardiac remodeling.

DOI: 10.26355/eurrev_202006_21525
	Sample: Cell culture supernatant

LIFE SCIENCES (2018) IF: 3.234

Inhibiting autophagy promotes collagen degradation by regulating matrix metalloproteinases in pancreatic stellate cells

DOI: 10.1016/j.lfs.2018.07.049
PMID: 30056017	Sample: Cell culture supernatant

NEUROCHEMISTRY INTERNATIONAL (2016) IF: 3.385

Study of synergistic role of allogenic skin-derived precursor differentiated Schwann cells and heregulin-1β in nerve regeneration with an acellular nerve allograft

DOI: 10.1016/j.neuint.2016.04.003
	Sample: Tissue homogenate

STARCH-STARKE (2018) IF: 2.173

Anti-Adhesion Mesh for Hernia Repair Based on Modified Bacterial Cellulose

DOI: 10.1002/star.201700319
	Sample: fibroblast cells (RFL-6 cells)

BIOTECHNIC & HISTOCHEMISTRY (2020) IF: 1.203

Effects of cannabinoid receptor 2 synthetic agonist, AM1241, on bleomycin induced pulmonary fibrosis

DOI: 10.1080/10520295.2020.1758343
	Sample: Tissue homogenate

Veterinary World (2021)

Wound healing potential of human umbilical cord mesenchymal stem cell conditioned medium: An in vitro and in vivo study in diabetes-induced rats

DOI: 10.14202/vetworld.2021.2109-2117
	Sample: Fibroblasts

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha