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Rat COL3(Collagen Type Ⅲ) ELISA Kit

  • Cat.No.:E-EL-R0235

  • Reactivity: Rat

To Purchase E-EL-R0235

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat COL3 in samples. No significant cross-reactivity or interference between Rat COL3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat COL3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL3. You can calculate the concentration of Rat COL3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat COL3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat COL3 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 216.81 334.18 1952.24 213.45 338.11 1998.02
Standard deviation 12.49 17.61 92.93 11.78 20.22 64.74
CV (%) 5.76 5.27 4.76 5.52 5.98 3.24


The recovery of Rat COL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 95
EDTA plasma (n=8) 94-106 100
Cell culture media (n=8) 94-109 101


Samples were spiked with high concentrations of Rat COL3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

  1. International Journal of Nanomedicine (2022) IF: 6.4
    Direct Detection of Pulmonary Fibrosis by Near-Infrared-Responsive Biomimetic Platelets

    DOI: 10.2147/IJN.S334331

    PMID: 35046651

    Sample: lung tissue
  2. Frontiers in Medicine (2022) IF: 5.058
    Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.

    DOI: 10.3389/fmed.2022.953749

    Sample: renal tissue
  3. PHYTOMEDICINE (2021) IF: 5.34
    Effective-components combination improves airway remodeling in COPD rats by suppressing M2 macrophage polarization via the inhibition of mTORC2 activity

    DOI: 10.1016/j.phymed.2021.153759

    PMID: 34600177

    Sample: bronchoalveolar lavage fluid (BALF)
  4. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    Effects of Secreted frizzled-related protein 1 on inhibiting cardiac remodeling.

    DOI: 10.26355/eurrev_202006_21525

    Sample: Cell culture supernatant
  5. LIFE SCIENCES (2018) IF: 3.234
    Inhibiting autophagy promotes collagen degradation by regulating matrix metalloproteinases in pancreatic stellate cells

    DOI: 10.1016/j.lfs.2018.07.049

    PMID: 30056017

    Sample: Cell culture supernatant
    Study of synergistic role of allogenic skin-derived precursor differentiated Schwann cells and heregulin-1β in nerve regeneration with an acellular nerve allograft

    DOI: 10.1016/j.neuint.2016.04.003

    Sample: Tissue homogenate
  7. STARCH-STARKE (2018) IF: 2.173
    Anti-Adhesion Mesh for Hernia Repair Based on Modified Bacterial Cellulose

    DOI: 10.1002/star.201700319

    Sample: fibroblast cells (RFL-6 cells)
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