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Rat CRP(C-Reactive Protein) ELISA Kit

Citations(48)

  • Cat.No.:E-EL-R0506

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat CRP in samples. No significant cross-reactivity or interference between Rat CRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat CRP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CRP. You can calculate the concentration of Rat CRP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CRP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.40 4.80 0.50 1.40 4.60
Standard deviation 0.03 0.06 0.24 0.03 0.08 0.22
CV (%) 6.00 4.29 5.00 6.00 5.71 4.78

Recovery

The recovery of Rat CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 94
EDTA plasma (n=8) 84-96 91
Cell culture media (n=8) 86-99 92

Linearity

Samples were spiked with high concentrations of Rat CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
    Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway

    DOI: 10.1016/j.biopha.2023.114330
    Sample: serum
  2. JOURNAL OF PINEAL RESEARCH (2014) IF: 7.812
    Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet‐induced metabolic syndrome in rats

    DOI: 10.1111/jpi.12168
    Sample: Plasma
  3. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    The dual gastro- and neuroprotective effects of curcumin loaded chitosan nanoparticles against cold restraint stress in rats

    DOI: 10.1016/j.biopha.2022.112778

    PMID: 35272135

    		 Sample: brain tissue
  4. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Lipoic acid (LA) dose-dependently protects bone losses in the mandible of rats during the development of osteopenia by inhibiting oxidative stress and promoting bone formation

    DOI: 10.1016/j.biopha.2021.112467

    PMID: 34891114

    		 Sample: serum
  5. Nutrients (2023) IF: 6.706
    Photoperiod Conditions Modulate Serum Oxylipins Levels in Healthy and Obese Rats: Impact of Proanthocyanidins and Gut Microbiota

    DOI: 10.3390/nu15030707
    Sample: serum
  6. Journal of Inflammation Research (2022) IF: 6.922
    Gastroprotective Effect of Azilsartan Through Ameliorating Oxidative Stress, Inflammation, and Restoring Hydroxyproline, and Gastrin Levels in Ethanol-Induced Gastric Ulcer

    DOI: 10.2147/JIR.S365090

    PMID: 35592072

    		 Sample: serum
  7. Journal of Inflammation Research (2021) IF: 6.922
    Anti-Inflammatory Potentials of the n-Hexane Fraction of Alstonia boonei Stem Bark in Lipopolysaccharide-Induced Inflammation in Wistar Rats

    DOI: 10.2147/JIR.S304076
    Sample: serum
  8. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Validation of traditional claims of anti-arthritic efficacy of trans-Himalayan snow mountain garlic (Allium ampeloprasum L.) extract using adjuvant-induced arthritis rat model: A comparative evaluation with normal garlic (Allium sativum L.) and dexamethas

    DOI: 10.1016/j.jep.2022.115939

    PMID: 36435406

    		 Sample: serum
  9. MOLECULAR NUTRITION & FOOD RESEARCH (2021) IF: 5.82
    Effects of High Intakes of Fructose and Galactose, with or without Added Fructooligosaccharides, on Metabolic Factors, Inflammation, and Gut Integrity in a Rat Model

    DOI: 10.1002/mnfr.202001133

    PMID: 33548087

    		 Sample: Serum
  10. INFLAMMATION (2021) IF: 4.092
    Neuropeptide W Attenuates Oxidative Multi-Organ Injury in Rats Induced with Intra-Abdominal Sepsis

    DOI: 10.1007/s10753-021-01545-5
    Sample: serum
  11. Nutrition & Metabolism (2021) IF: 4.169
    Diet-induced prediabetes: effects of exercise treatment on risk factors for cardiovascular complications

    DOI: 10.1186/s12986-021-00573-0

    PMID: 33888141

    		 Sample: Tissue homogenate(heart)
  12. JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION (2022) IF: 4.256
    Body, metabolic and renal changes following cross-sex estrogen/progestogen therapy in a rodent model simulating its use by transwomen

    DOI: 10.1007/s40618-022-01817-3

    PMID: 35689728

    		 Sample: serum
  13. Foods (2021) IF: 4.35
    Quality of Dabai Pulp Oil Extracted by Supercritical Carbon Dioxide and Supplementation in Hypercholesterolemic Rat—A New Alternative Fat

    DOI: 10.3390/foods10020262
    Sample: Serum
  14. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats

    DOI: 10.1016/j.ejphar.2021.174044

    PMID: 33745959

    		 Sample: Tissue homogenate(Joint Tissue)
  15. Nutrients (2020) IF: 4.546
    Effect of Defatted Dabai Pulp Extract in Urine Metabolomics of Hypercholesterolemic Rats

    DOI: 10.3390/nu12113511

    PMID: 33202660

    		 Sample: Serum
  16. International Journal of Environmental Research and Public Health (2022) IF: 4.614
    Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid

    DOI: 10.3390/ijerph19137921

    PMID: 35805580

    		 Sample: plasma
  17. Biomedicines (2022) IF: 4.757
    Kinetin Ameliorates Cisplatin-Induced Hepatotoxicity and Lymphotoxicity via Attenuating Oxidative Damage, Cell Apoptosis and Inflammation in Rats

    DOI: 10.3390/biomedicines10071620

    PMID: 35884925

    		 Sample: lymphocytes
  18. Scientific Reports (2022) IF: 4.996
    Network pharmacology combined with pharmacodynamics revealed the anti-inflammatory mechanism of Tanreqing capsule against acute-exacerbation chronic obstructive pulmonary disease

    DOI: 10.1038/s41598-022-18326-1

    PMID: 35978041

    		 Sample: serum
  19. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172786

    PMID: 31712060

    		 Sample: Serum
  20. PLoS One (2022) IF: 3.24
    The effect of TNF-α inhibitor treatment on microRNAs and endothelial function in collagen induced arthritis

    DOI: 10.1371/journal.pone.0264558

    PMID: 35213638

    		 Sample: serum
  21. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2019) IF: 3.319
    Cardioprotective effects of a ruthenium (II) Schiff base complex in diet-induced prediabetic rats

    DOI: 10.2147/DMSO.S183811
    Sample: Tissue homogenate,Plasma
  22. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023) IF: 3.322
    Acupuncture exerts preventive effects in rats of chronic unpredictable mild stress: The involvement of inflammation in amygdala and brain-spleen axis

    DOI: 10.1016/j.bbrc.2023.01.046
    Sample: Serum,amygdala
  23. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    Gynura procumbens ethanol extract improves vascular dysfunction by suppressing inflammation in postmenopausal rats fed a high-fat diet

    DOI: 10.1080/13880209.2021.1970199

    PMID: 34493166

    		 Sample: plasma
  24. Journal of Functional Foods (2020) IF: 3.701
    Regulation of inflammatory response and oxidative stress by tocotrienol in a rat model of non-alcoholic fatty liver disease

    DOI: 10.1016/j.jff.2020.104209
    Sample: serum and liver
  25. IUBMB LIFE (2021) IF: 3.885
    Endogenous dipeptide-carnosine supplementation ameliorates hypobaric hypoxia-induced skeletal muscle loss via attenuating endoplasmic reticulum stress response and maintaining proteostasis

    DOI: 10.1002/iub.2539

    PMID: 34455667

    		 Sample: serum
  26. Cancer Management and Research (2021) IF: 3.989
    Cardioprotective Effect of Quercetin and Sitagliptin in Doxorubicin-Induced Cardiac Toxicity in Rats

    DOI: 10.2147/CMAR.S300495

    PMID: 33737832

    		 Sample: Serum
  27. BMC Complementary and Alternative Medicine (2015) IF: 2.02
    Edible Bird’s Nest attenuates high fat diet-induced oxidative stress and inflammation via regulation of hepatic antioxidant and inflammatory genes

    DOI: 10.1186/s12906-015-0843-9
    Sample: Serum
  28. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019) IF: 2.041
    The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2019-0369

    PMID: 31491333

    		 Sample: Serum,Plasma,Tissue homogenate
  29. Therapeutic Advances in Endocrinology and Metabolism (2020) IF: 2.173
    Plant-derived oleanolic acid ameliorates markers of subclinical inflammation and innate immunity activation in diet-induced pre-diabetic rats:

    DOI: 10.1177/2042018820935771
    Sample: Plasma
  30. BMC Cardiovascular Disorders (2022) IF: 2.174
    Increased protein phosphatase 5 expression in inflammation-induced left ventricular dysfunction in rats

    DOI: 10.1186/s12872-022-02977-z
    Sample: serum
  31. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.21
    The pectin–insulin patch application prevents the onset of peripheral neuropathy-like symptoms in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2018-0415
    Sample: Plasma
  32. AUTOIMMUNITY (2019) IF: 2.403
    The changes in immune cell concentration during the progression of pre-diabetes to type 2 diabetes in a high-fat high-carbohydrate diet-induced pre-diabetic rat model

    DOI: 10.1080/08916934.2019.1575820

    PMID: 30776930

    		 Sample: Plasma
  33. Journal of Ovarian Research (2019) IF: 2.469
    Abnormal expression of HSP70 may contribute to PCOS pathology

    DOI: 10.1186/s13048-019-0548-7
    Sample: serum
  34. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Combination of Chinese and Western Medicine Optimizes the Intestinal Microbiota of Exacerbated Chronic Obstructive Pulmonary Disease in Rats

    DOI: 10.1155/2021/9975407

    PMID: 34539809

    		 Sample: serum
  35. BIOMARKERS (2022) IF: 2.663
    Justicia carnea extracts ameliorated hepatocellular damage in streptozotocin-induced type 1 diabetic male rats via decrease in oxidative stress, inflammation and increasing other risk markers

    DOI: 10.1080/1354750X.2022.2157487
    Sample: liver
  36. PLoS One (2020) IF: 2.74
    Associations of inflammatory markers with impaired left ventricular diastolic and systolic function in collagen-induced arthritis

    DOI: 10.1371/journal.pone.0230657

    PMID: 32208438

    		 Sample: Serum
  37. BIOMEDICINE & PHARMACOTHERAPY (2017) IF: 2.759
    Anti-steatotic and anti-inflammatory effects of Hovenia dulcis Thunb. extracts in chronic alcohol-fed rats

    DOI: 10.1016/j.biopha.2017.03.077
    Sample: Serum
  38. JOURNAL OF BIOMEDICAL SCIENCE (2015) IF: 2.763
    High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats

    DOI: 10.1186/s12929-015-0211-6
    Sample: Serum
  39. Journal of Ovarian Research (2020) IF: 2.989
    The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats.

    DOI: 10.1186/s13048-020-00650-7
    Sample: Serum
  40. Annals of Surgical Treatment and Research (2019) IF: 1.181
    Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

    DOI: 10.4174/astr.2019.97.6.271

    PMID: 31824881

    		 Sample: Serum,Tissue homogenate
  41. Annals of Surgical Treatment and Research (2022) IF: 1.859
    Comparison of the healing effects of mesazaline and Ganoderma lucidum in acetic acid-induced colitis in rats

    DOI: 10.4174/astr.2022.102.1.29

    PMID: 35071117

    		 Sample: colonic tissue
  42. XENOBIOTICA (2021) IF: 1.908
    Pharmacokinetics, distribution and efficacy of triptolide PLGA microspheres after intra-articular injection in a rat rheumatoid arthritis model

    DOI: 10.1080/00498254.2021.1923860

    PMID: 33938387

    		 Sample: Serum,Synovia
  43. PHARMACEUTICAL BIOLOGY (2018) IF: 1.918
    Mechanism of TangGanJian on nonalcoholic fatty liver disease with type 2 diabetes mellitus

    DOI: 10.1080/13880209.2018.1504972
    Sample: serum
  44. Physiological Reports (2021)
    Inflammation-induced left ventricular fibrosis is partially mediated by tumor necrosis factor-α

    DOI: 10.14814/phy2.15062
    Sample: serum
  45. Heliyon (2021)
    Changes in antioxidants status, atherogenic index and cardiovascular variables after prolonged doses of D-ribose-L-cysteine in male Wistar rats

    DOI: 10.1016/j.heliyon.2021.e06287
    Sample: plasma
  46. Advances in Traditional Medicine (2023)
    Amaranthus spinosus (Spiny Pigweed) methanol leaf extract alleviates oxidative and inflammation induced by doxorubicin in male sprague dawley rats

    DOI: 10.1007/s13596-022-00677-9
    Sample: serum
  47. Scientific African (2022)
    Allium vineale methanol extract attenuated oxidative stress and inflammation induced by doxorubicin in Sprague Dawley Rats

    DOI: 10.1016/j.sciaf.2022.e01244
    Sample: serum
  48. Asian Pacific Journal of Tropical Medicine (2016) IF: 0.841
    Asiatic acid-pectin hydrogel matrix patch transdermal delivery system influences parasitaemia suppression and inflammation reduction in P. berghei murine malaria infected Sprague–Dawley rats

    DOI: 10.1016/j.apjtm.2016.10.008
    Sample: Plasma,Tissue homogenate
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