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Rat EGF(Epidermal Growth Factor) ELISA Kit

  • Cat.No.:E-EL-R0369

  • Reactivity: Rat

To Purchase E-EL-R0369

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat EGF in samples. No significant cross-reactivity or interference between Rat EGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat EGF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EGF. You can calculate the concentration of Rat EGF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat EGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat EGF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 55.10 94.40 445.80 57.60 94.60 475.90
Standard deviation 3.40 5.30 15.20 3.20 5.60 19.00
CV (%) 6.17 5.61 3.41 5.56 5.92 3.99


The recovery of Rat EGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 92
EDTA plasma (n=8) 93-107 99
Cell culture media (n=8) 93-107 100


Samples were spiked with high concentrations of Rat EGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsURG, HOMG4, Beta-Urogastrone
Research AreaCancer, Cell Biology, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

    Photo-responsive supramolecular hyaluronic acid hydrogels for accelerated wound healing

    DOI: 10.1016/j.jconrel.2020.04.014

    PMID: 32283209

    Sample: Tissue homogenate
  2. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    Sample: serum
  3. Scientific Reports (2021) IF: 4.38
    A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer

    DOI: 10.1038/s41598-021-04008-x

    PMID: 34961769

    Sample: serum
  4. PLANTA MEDICA (2022) IF: 3.007
    Curative effect and mechanisms of Radix Arnebiae oil on burn wound healing in rats

    DOI: 10.1055/a-1997-5566

    Sample: burn wound tissue
    Impact of repeated intravenous infusions of umbilical cord–derived versus bone marrow–derived mesenchymal stem cells on angiogenesis in a pregnant experimentally induced deep venous thrombosis rat model

    DOI: 10.1007/s11626-022-00733-3

    Sample: serum
  6. Annals of Surgical Treatment and Research (2019) IF: 1.181
    Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

    DOI: 10.4174/astr.2019.97.6.271

    PMID: 31824881

    Sample: Serum,Tissue homogenate
  7. Annals of Transplantation (2021) IF: 1.53
    Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia

    DOI: 10.12659/AOT.928047

    PMID: 33414361

    Sample: The amniotic fluid samples
  8. Asian Journal of Surgery (2019) IF: 1.56
    Potent therapeutic effects of ruscogenin on gastric ulcer established by acetic acid

    DOI: 10.1016/j.asjsur.2019.07.001

    PMID: 31345657

    Sample: Tissue homogenate
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Verified Customer

h*********aSubmitted [ Jun 17 2019 ]

  • Species:Human
  • Sample:Rat urine
  • Sample source:Rat urine
  • Description:The ELISA Kit of Rat EGF I bought to detect Rat urine . The result is helpful to my subject . Rat EGF concentration of urine is high . it’s more sensitive than serum value . In the process , CSD help me a lot . thy are so patient . although I made a mistake at first . they found it and correct it .I got the satisfying answer in final . I am very grateful .
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