Rat FETUA(Fetuin A) ELISA Kit (E-EL-R3059)

Name: Rat FETUA(Fetuin A) ELISA Kit
Brand: Elabscience
SKU: E-EL-R3059
Price: 150.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	30.51 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat FETUA in samples. No significant cross-reactivity or interference between Rat FETUA and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	2.288	2.192	2.240	2.157
2500	1.609	1.655	1.632	1.549
1250	0.980	0.956	0.968	0.885
625	0.551	0.555	0.553	0.470
312.5	0.304	0.312	0.308	0.225
156.25	0.178	0.186	0.182	0.099
78.13	0.133	0.139	0.136	0.053
0	0.082	0.084	0.083	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	232.050	532.480	1704.410	248.730	515.320	1543.910
Standard deviation	12.390	30.780	71.410	20.890	43.800	131.700
CV（%）	5.340	5.780	4.190	8.400	8.500	8.530

Recovery

The recovery of Rat FETUA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-110	102
EDTA plasma （n=8）	88-101	94
Cell culture media （n=8）	94-107	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat FETUA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-116	94-108	91-104
1:2	Average	106	99	96
1:4	Range	100-117	93-106	89-103
1:4	Average	108	100	96
1:8	Range	101-113	91-103	91-106
1:8	Average	107	97	97

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	87.99-100.93
Sample 2（n=16）	95.04-100.00

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FETUA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FETUA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FETUA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FETUA. You can calculate the concentration of Rat FETUA in the samples by comparing the OD of the samples to the standard curve.

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