Rat FGF23(Fibroblast Growth Factor 23) ELISA Kit (E-EL-R3031)

Name: Rat FGF23(Fibroblast Growth Factor 23) ELISA Kit
Brand: Elabscience
SKU: E-EL-R3031
Price: 150.0 USD
Availability: InStock

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Rat FGF23(Fibroblast Growth Factor 23) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat FGF23 in samples.No significant cross-reactivity or interference between Rat FGF23 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.196	2.150	2.173	2.094
2000	1.530	1.578	1.554	1.475
1000	0.821	0.841	0.831	0.752
500	0.428	0.412	0.420	0.341
250	0.277	0.283	0.280	0.201
125	0.172	0.164	0.168	0.089
62.5	0.131	0.125	0.128	0.049
0	0.078	0.080	0.079	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	210.990	552.600	1592.580	210.510	523.540	1654.930
Standard deviation	12.620	27.460	93.800	13.510	34.080	134.710
CV（%）	5.980	4.970	5.890	6.420	6.510	8.140

Recovery

The recovery of Rat FGF23 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-105	102
EDTA plasma （n=8）	96-111	104
Cell culture media （n=8）	89-95	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat FGF23 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-101	94-104	85-98
1:2	Average	96	98	92
1:4	Range	98-109	88-101	88-100
1:4	Average	103	94	92
1:8	Range	101-109	94-109	102-112
1:8	Average	104	101	105

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	105.99-106.99
Sample 2（n=16）	98.54-103.20

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FGF23. You can calculate the concentration of Rat FGF23 in the samples by comparing the OD of the samples to the standard curve.

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