Rat FSH(Follicle Stimulating Hormone) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-R0391

      Synonyms:FSH

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Rat

      Detection Range: 3.13~200 ng/mL

      Sensitivity: 1.88 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Rat FSH concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FSH. You can calculate the concentration of Rat FSH in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Rat
      Detection Method Colormetric
      Detection Range 3.13—200 ng/mL
      Sensitivity 1.88 ng/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Rat FSH in samples. No significant cross-reactivity or interference between Rat FSH and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (ng/mL)
      O.D Average Corrected
      200 2.357
      2.413
      2.385 2.299
      100 1.551
      1.563
      1.557 1.471
      50 0.895
      0.869
      0.882 0.796
      25 0.391
      0.421
      0.406 0.32
      12.5 0.245
      0.227
      0.236 0.15
      6.25 0.184
      0.166
      0.175 0.089
      3.13 0.127
      0.139
      0.133 0.047
      0 0.078
      0.094
      0.086 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat FSH were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat FSH were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 10.50 22.00 75.70 10.90 21.50 75.20
      Standard deviation 0.70 1.10 3.30 0.70 1.30 3.80
      C V (%) 6.67 5.00 4.36 6.42 6.05 5.05

      Recovery

      The recovery of Rat FSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 92-102 97
      EDTA plasma (n=5) 90-106 97
      Cell culture media (n=5) 88-102 96

      Linearity

      Samples were spiked with high concentrations of Rat FSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 96-110 87-103 95-109
      Average (%) 101 94 102
      1:4 Range (%) 101-117 81-94 97-110
      Average (%) 107 87 104
      1:8 Range (%) 100-115 86-98 100-110
      Average (%) 106 91 105
      1:16 Range (%) 98-113 82-95 91-106
      Average (%) 105 87 97

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.
        IF: 2.163
        Journal: Journal of Assisted Reproduction & Genetics(2015)
        DOI: 10.1007/s10815-015-0506-2
        PMID: 26081124
        Products: E-EL-0150 E-EL-0155 E-EL-R0391 E-EL-R0026 E-EL-R0582 
        Sample type: Serum
        Reactivity: Rat
      2. Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.
        IF: 1.692
        Journal: Molecular Medicine Reports(2016)
        DOI: 10.3892/mmr.2016.5712
        PMID: 27600729
        Products: E-EL-0150 E-EL-0155 E-EL-R0391 E-EL-R0026 E-EL-R0582 
        Sample type: Serum
        Reactivity: Rat
      3. Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR.
        IF: 5.104
        Journal: Cellular Physiology and Biochemistry(2017)
        DOI: 10.1159/000457881
        PMID: 28214901
        Products: E-EL-R0026 E-EL-R0391 E-EL-0155 E-EL-0150 
        Sample type: Serum
        Reactivity: Rat
      4. Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.
        IF: 1.692
        Journal: Molecular Medicine Reports(2016)
        DOI: 10.3892/mmr.2016.5712
        PMID: 27600729
        Products: E-EL-0150 E-EL-0155 E-EL-R0391 E-EL-R0026 E-EL-R0582 
        Sample type: Serum
        Reactivity: Rat
      5. The Anorectic Agent, Lorcaserin, Disturbs Estrous Cyclicity And Produces Endometrial Hyperplasia Without Affecting Ovarian Population In Female Rats.
        IF: 2.936
        Journal: Life Sciences(2017)
        DOI: 10.1016/j.lfs.2017.06.006
        PMID: 28599864
        Products: E-EL-R0391 
        Sample type: Serum
        Reactivity: Rat
      6. ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.
        IF: 5.965
        Journal: Cell Death & Disease(2017)
        DOI: 10.1038/cddis.2017.494
        PMID: 29072679
        Products: E-EL-0154 E-EL-0155 E-EL-0150 E-EL-H0116 E-EL-H1143 E-EL-H0532 E-EL-R0026 E-EL-R0391 E-EL-R0017 
        Sample type: Serum
        Reactivity: Universal
      7. Rescue Effects Of Aqueous Seed Extracts Of Foeniculum Vulgare And Carum Carvi Against Cadmium-Induced Hepatic, Renal And Gonadal Damage In Female Albino Rats.
        IF: 0.925
        Journal: Asian Pacific Journal of Tropical Medicine(2017)
        DOI: 10.1016/j.apjtm.2017.10.019
        PMID: 29268967
        Products: E-EL-R0391 E-EL-R0026 
        Sample type: Serum
        Reactivity: Rat
      8. Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats.
        IF: 2.225
        Journal: Autonomic Neuroscience-Basic & Clinical(2015)
        DOI: 10.1016/j.autneu.2015.02.005
        PMID: 25784152
        Products: E-EL-R0391 E-EL-0071 E-EL-R0026 E-EL-0155 
        Sample type: Plasma
        Reactivity: Rat
      9. GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile.
        IF: 2.849
        Journal: Reproductive Biology and Endocrinology(2018)
        DOI: 10.1186/s12958-018-0354-x
        PMID: 29642911
        Products: E-EL-R0391 E-EL-R0026 
        Reactivity: Rat
      10. Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging.
        IF: 2.341
        Journal: Physiology & behavior(2018)
        DOI: 10.1016/j.physbeh.2018.03.007
        PMID: 29526572
        Products: E-EL-R0391 
        Reactivity: Rat
      11. Sleeve Gastrectomy Reversed Obesity-Induced Hypogonadism in a Rat Model by Regulating Inflammatory Responses in the Hypothalamus and Testis.
        IF: 3.947
        Journal: Obesity Surgery(2018)
        DOI: 10.1007/s11695-018-3150-y
        PMID: 29508270
        Products: E-EL-R0391 
        Reactivity: Rat
      12. Melatonin prevents and ameliorates lead-induced gonadotoxicity through antioxidative and hormonal mechanisms.
        IF: 1.255
        Journal: Toxicology and industrial health(2018)
        DOI: 10.1177/0748233718773508
        PMID: 29759042
        Products: E-EL-R0597 E-EL-R0391 
        Sample type: Plasma
        Reactivity: Rat
      13. GnRH dysregulation in polycystic ovarian syndrome (PCOS) is a manifestation of an altered neurotransmitter profile.
        IF: 2.849
        Journal: Reproductive Biology and Endocrinology(2018)
        DOI: 10.1186/s12958-018-0354-x
        PMID: 29642911
        Products: E-EL-R0391 E-EL-R0026 
        Sample type: Serum
        Reactivity: Rat
      14. Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy.
        IF: 3.259
        Journal: Andrology(2018)
        DOI: 10.1111/andr.12490
        PMID: 29654715
        Products: E-EL-R0389 E-EL-R0026 E-EL-R0391 
        Sample type: Serum
        Reactivity: Rat
      15. Expression of FKBP52 in the ovaries of PCOS rats
        IF: 2.784
        Journal: International Journal of Molecular Medicine(2018)
        DOI: 10.3892/ijmm.2018.3998
        PMID: 30483787
        Products: E-EL-R0391 E-EL-R0026 E-EL-0150 E-EL-0154 E-EL-0155 
        Sample type: Serum
        Reactivity: Rat
      16. Effects of chlorocholine chloride on pubertal development and reproductive functions in male rats
        IF: 3.499
        Journal: Toxicology Letters(2019)
        DOI: 10.1016/j.toxlet.2019.10.024
        PMID: 31689472
        Products: E-EL-R2530 E-EL-0071 E-EL-R0391 E-EL-R0026 
        Sample type: Serum
        Reactivity: Rat
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