Current Position:Home>>Kits>>ELISA Kits>> Rat GH(Growth Hormone) ELISA Kit

Rat GH(Growth Hormone) ELISA Kit

Cat.No.:E-EL-R3003
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R3003

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat GH in samples. No significant cross-reactivity or interference between Rat GH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat GH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GH. You can calculate the concentration of Rat GH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.43	10.28	41.61	4.92	12.26	43.56
Standard deviation	0.23	0.47	1.92	0.28	0.58	2.06
CV (%)	5.19	4.57	4.61	5.69	4.73	4.73

Recovery

The recovery of Rat GH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-99	91
EDTA plasma (n=8)	90-102	98
Cell culture media (n=8)	92-106	99

Linearity

Samples were spiked with high concentrations of Rat GH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	GH1, GH-N, GHN, Somatotropin
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

DIABETES (2017) IF: 9.461

Inorganic Nitrate Mimics Exercise-Stimulated Muscular Fiber-Type Switching and Myokine and γ-Aminobutyric Acid Release

DOI: 10.2337/db16-0843
	Sample: Plasma

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129

Targeting the lncMST-EPRS/HSP90AB1 complex as novel therapeutic strategy for T-2 toxin-induced growth retardation

DOI: 10.1016/j.ecoenv.2022.114243
	Sample: blood

Computational and Structural Biotechnology Journal (2020) IF: 6.018

Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

DOI: 10.1016/j.csbj.2020.11.009
PMID: 33304464	Sample: blood

FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025

PPAR-γ with its anti-fibrotic action could serve as an effective therapeutic target in T-2 toxin-induced cardiac fibrosis of rats

DOI: 10.1016/j.fct.2021.112183
PMID: 33836209	Sample: Plasma

JOURNAL OF MOLECULAR ENDOCRINOLOGY (2021) IF: 5.098

Pathological changes and oxidative stress of the HPG axis in hypothyroid rat

DOI: 10.1530/JME-21-0095
PMID: 34270447	Sample: serum

GROWTH HORMONE & IGF RESEARCH (2022) IF: 2.372

Effects of high-dose all-trans retinoic acid on longitudinal bone growth of young rats

DOI: 10.1016/j.ghir.2022.101446
PMID: 35149382	Sample: serum

MICROSCOPY AND MICROANALYSIS (2019) IF: 2.673

Bee Venom Stimulates Hormone Secretion in Rat Somatotroph and Corticotroph Cells: Digital Image Analysis of Secretory Granules

DOI: 10.1017/S1431927619014910
	Sample: serum

YONSEI MEDICAL JOURNAL (2018) IF: 1.564

Effect of the Orally Active Growth Hormone Secretagogue MK-677 on Somatic Growth in Rats

DOI: 10.3349/ymj.2018.59.10.1174
PMID: 30450851	Sample: Serum,Tissue homogenate

World Neurosurgery (2019) IF: 1.723

AFAP1-AS1 Promotes Proliferation of Pituitary Adenoma Cells through miR-103a-3p to Activate PI3K/AKT Signaling Pathway

DOI: 10.1016/j.wneu.2019.07.032
	Sample: GH3 cells,MMQ cells

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha