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Rat GLP-1(Glucagon Like Peptide 1) ELISA Kit

Cat.No.:E-EL-R3007
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 pg/mL
Sensitivity	0.94 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat GLP-1 in samples. No significant cross-reactivity or interference between Rat GLP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat GLP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GLP-1. You can calculate the concentration of Rat GLP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GLP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GLP-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	4.19	10.25	48.72	4.77	10.96	47.45
Standard deviation	0.23	0.50	2.43	0.26	0.52	2.43
CV (%)	5.49	4.88	4.99	5.45	4.74	5.12

Recovery

The recovery of Rat GLP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-103	99
EDTA plasma (n=8)	86-97	92
Cell culture media (n=8)	83-97	91

Linearity

Samples were spiked with high concentrations of Rat GLP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	GLP1
Research Area	Cardiovascular, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

MOLECULAR NEUROBIOLOGY (2017) IF: 6.19

Brain GLP-1/IGF-1 Signaling and Autophagy Mediate Exendin-4 Protection Against Apoptosis in Type 2 Diabetic Rats

DOI: 10.1007/s12035-017-0622-3
	Sample: Tissue homogenate

JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195

Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway

DOI: 10.1016/j.jep.2022.115862
PMID: 36283638	Sample: serum

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219

Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage

DOI: 10.1016/j.sjbs.2021.07.010
PMID: 34764759	Sample: brain

PHYTOMEDICINE (2020) IF: 4.268

Cyclocarya paliurus polysaccharides alleviate type 2 diabetic symptoms by modulating gut microbiota and short-chain fatty acids

DOI: 10.1016/j.phymed.2020.153268
PMID: 32663709	Sample: Serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556

Liraglutide Protects Against Brain Amyloid-β1–42 Accumulation in Female Mice with Early Alzheimer’s Disease-Like Pathology by Partially Rescuing Oxidative/Nitrosative Stress and Inflammation

DOI: 10.3390/ijms21051746
	Sample: brain cortical

EUROPEAN JOURNAL OF NUTRITION (2020) IF: 4.664

Renoprotective effects of vitamin D3 supplementation in a rat model of metabolic syndrome

DOI: 10.1007/s00394-020-02249-6
	Sample: serum

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2019) IF: 3.532

Vitamin D3 potentiates the renoprotective effects of vildagliptin in a rat model of fructose/salt-induced insulin resistance

DOI: 10.1016/j.ejps.2019.105196
	Sample: serum

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2019) IF: 3.571

Antiobesity Effect of Flaxseed Polysaccharide via Inducing Satiety due to Leptin Resistance Removal and Promoting Lipid Metabolism through the AMP-Activated Protein Kinase (AMPK) Signaling Pathway

DOI: 10.1021/acs.jafc.9b02434
	Sample: serum

Heliyon (2022) IF: 3.776

Mangrove fruit (Bruguiera gymnorhiza) increases circulating GLP-1 and PYY, modulates lipid profiles, and reduces systemic inflammation by improving SCFA levels in obese wistar rats

DOI: 10.1016/j.heliyon.2022.e10887
	Sample: blood,caecum

Integrative Medicine Research (2019) IF: 2.379

Orthosiphon stamineus as a potential antidiabetic drug in maternal hyperglycemia in streptozotocin-induced diabetic rats

DOI: 10.1016/j.imr.2019.05.006
	Sample: plasm

Dose-Response (2022) IF: 2.623

Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :

DOI: 10.1177/15593258221114184
	Sample: serum,Pancreatic,intestinal tissue

Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63

Ge-Gen-Jiao-Tai-Wan Affects Type 2 Diabetic Rats by Regulating Gut Microbiota and Primary Bile Acids

DOI: 10.1155/2021/5585952
PMID: 33953783	Sample: Serum

BIOTECHNIC & HISTOCHEMISTRY (2021) IF: 1.718

Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones

DOI: 10.1080/10520295.2021.1896780
PMID: 33722110	Sample: serum

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2020) IF: 1.946

Vitamin D3 potentiates the nephroprotective effects of metformin in a rat model of metabolic syndrome: role of AMPK/SIRT1 activation and DPP-4 inhibition

DOI: 10.1139/cjpp-2020-0435
	Sample: serum

Vascular Health and Risk Management (2023)

The Effectiveness of Nigella sativa and Ginger as Appetite Suppressants: An Experimental Study on Healthy Wistar Rats

DOI: 10.2147/VHRM.S396295
	Sample: serum

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