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Rat GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit

  • Cat.No.:E-EL-R0008

  • Reactivity: Rat

To Purchase E-EL-R0008

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat GM-CSF in samples. No significant cross-reactivity or interference between Rat GM-CSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat GM-CSF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GM-CSF. You can calculate the concentration of Rat GM-CSF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat GM-CSF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat GM-CSF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.74 130.81 345.85 50.91 121.73 371.14
Standard deviation 2.61 5.57 11.27 3.22 4.89 13.14
CV (%) 5.25 4.26 3.26 6.32 4.02 3.54


The recovery of Rat GM-CSF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 98
EDTA plasma (n=8) 87-100 93
Cell culture media (n=8) 91-103 97


Samples were spiked with high concentrations of Rat GM-CSF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsGMCSF, CSF2
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Inflammation (2020) IF: 3.212
    Sex-Based Differences in Monocytic Lineage Cells Contribute to More Severe Collagen-Induced Arthritis in Female Rats Compared with Male Rats

    DOI: 10.1007/s10753-020-01302-0

    PMID: 32857321

    Sample: Cell culture supernatant
  2. Mediators of Inflammation (2020) IF: 3.758
    Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD

    DOI: 10.1155/2020/4260204

    PMID: 32454790

    Sample: Tissue homogenate,Bronchoalveolar Lavage Fluid
  3. Mechanisms of Ageing And Development (2017) IF: 3.087
    Strain Specificities In Cellular And Molecular Immunopathogenic Mechanisms Underlying Development Of Experimental Autoimmune Encephalomyelitis In Aged Rats.

    DOI: 10.1016/j.mad.2017.03.001

    PMID: 28284714

    Sample: Cell culture supernatant
  4. Immunologic research (2019) IF: 2.507
    Noradrenaline modulates CD4+ T cell priming in rat experimental autoimmune encephalomyelitis: a role for the α 1-adrenoceptor

    DOI: 10.1007/s12026-019-09082-y

    PMID: 31396845

    Sample: Tissue homogenate
  5. International Immunopharmacology (2016) IF: 2.956
    Estradiol Enhances Capacity Of TLR-Matured Splenic Dendritic Cells To Polarize CD4+ Lymphocytes Into IL-17/GM-CSF-Producing Cells In Vitro.

    DOI: 10.1016/j.intimp.2016.09.001

    PMID: 27620506

    Sample: Cell culture supernatant
  6. Plos One (2016) IF: 2.806
    GM-CSF-Producing Th Cells In Rats Sensitive And Resistant To Experimental Autoimmune Encephalomyelitis.

    DOI: 10.1371/journal.pone.0166498

    PMID: 27832210

    Sample: Cell culture supernatant
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