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Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

  • Cat.No.:E-EL-R0513

  • Reactivity: Rat

To Purchase E-EL-R0513

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat HIF-1α in samples. No significant cross-reactivity or interference between Rat HIF-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat HIF-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HIF-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HIF-1α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 23.60 50.30 194.20 22.50 49.60 180.80
Standard deviation 1.30 2.70 6.20 1.20 2.10 6.30
CV (%) 5.51 5.37 3.19 5.33 4.23 3.48

Recovery

The recovery of Rat HIF-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 102
EDTA plasma (n=8) 87-99 93
Cell culture media (n=8) 85-99 90

Linearity

Samples were spiked with high concentrations of Rat HIF-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsHIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
Research AreaCancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Antioxidants (2022) IF: 7.675
    Effect of Empagliflozin on Thioacetamide-Induced Liver Injury in Rats: Role of AMPK/SIRT-1/HIF-1α Pathway in Halting Liver Fibrosis

    DOI: 10.3390/antiox11112152

    Sample: liver
  2. EUROPEAN JOURNAL OF PHARMACOLOGY (2023) IF: 5.195
    Linagliptin counteracts rotenone’s toxicity in non-diabetic rat model of Parkinson’s disease: Insights into the neuroprotective roles of DJ-1, SIRT-1/Nrf-2 and implications of HIF1-α

    DOI: 10.1016/j.ejphar.2023.175498

    Sample: midbrain,striatum
  3. CELL TRANSPLANTATION (2022) IF: 4.064
    Exosomes Derived From Mesenchymal Stem Cells Pretreated With Ischemic Rat Heart Extracts Promote Angiogenesis via the Delivery of DMBT1:

    DOI: 10.1177/09636897221102898

    Sample: heart tissue
  4. Drug Design Development and Therapy (2023) IF: 4.319
    Anti-Arthritic Effect of Edaravone Against Complete Freund Adjuvant Induced Arthritis via Osteoclast Differentiation and HIF-1α–VEGF–ANG-1 Axis

    DOI: 10.2147/DDDT.S391606

    Sample: serum
  5. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Investigation on the Q-markers of Bushen Huoxue Prescriptions for DR treatment based on chemometric methods and spectrum-effect relationship

    DOI: 10.1016/j.jep.2021.114800

    PMID: 34748867

    Sample: eyes
  6. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2021) IF: 3.377
    Metformin ameliorates bladder dysfunction in a rat model of partial bladder outlet obstruction

    DOI: 10.1152/ajprenal.00625.2020

    PMID: 33645317

    Sample: Tissue homogenate(Bladder)
  7. CELLULAR AND MOLECULAR NEUROBIOLOGY (2020) IF: 3.606
    Sulforaphane Induces Glioprotection After LPS Challenge

    DOI: 10.1007/s10571-020-00981-5

    PMID: 33079284

    Sample: Cell lysate
  8. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway

    DOI: 10.1111/jfbc.14397

    PMID: 36069470

    Sample: heart tissue
  9. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Alleviation of remote lung injury following liver ischemia/reperfusion: Possible protective role of vildagliptin

    DOI: 10.1016/j.intimp.2020.107305

    PMID: 33388732

    Sample: lung tissue
  10. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2019) IF: 2.058
    Protective effect of dipeptidyl peptidase-4 inhibitors in testicular torsion/detorsion in rats: a possible role of HIF-1α and nitric oxide

    DOI: 10.1007/s00210-019-01765-5

    Sample: testicular
  11. Molecular Medicine Reports (2022) IF: 2.952
    Reparative effects of chronic intermittent hypobaric hypoxia pre‑treatment on intervertebral disc degeneration in rats

    DOI: 10.3892/mmr.2022.12689

    PMID: 35315494

    Sample: serum
  12. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2020) IF: 0.504
    Effect of penehyclidine hydrochloride on IL-6, TNF-α, HIF- 1α and oxidative stress in lung tissue of young rats with acute lung injury

    DOI: 10.4314/tjpr.v19i7.14

    Sample: Serum
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