Rat HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit (E-EL-R0513)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Hypoxia-inducible factor 1 alpha (HIF-1alpha) is the oxygen-regulated alpha subunit of the transcription factor HIF-1, a heterodimer of alpha and beta subunits that serves as the principal molecular sensor of oxygen availability in mammalian cells. A member of the basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) family, it is synthesized continuously but rapidly degraded in normoxia through oxygen-dependent prolyl hydroxylation, ubiquitination and proteasomal destruction. Under hypoxia this degradation is suppressed, and the alpha subunit accumulates, translocates to the nucleus and dimerizes with the beta subunit. Once stabilized, HIF-1 acts as a master regulator of the hypoxic homeostatic response, activating genes that increase oxygen delivery and support metabolic adaptation, including vascular endothelial growth factor (VEGF), erythropoietin and glycolytic enzymes. It participates in embryonic vascularization, ischemic adaptation and ischemic disease, and alternatively spliced alpha-subunit isoforms with distinct regulatory properties have been identified. Because of its central role in oxygen sensing, HIF-1alpha is of major interest in cancer biology, where intratumoral hypoxia drives angiogenesis, metabolic reprogramming and therapy resistance, and it is also relevant to ischemia, wound healing and pulmonary hypertension. This ELISA kit enables quantitative determination of rat HIF-1alpha in cell and tissue lysates. In rat models it supports studies of hypoxic signaling, neovascularization and the response to hypoxic or ischemic challenge.