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Rat HO1(Heme Oxygenase 1) ELISA Kit

Citations(22)Uniprot : P06762

  • Cat.No.:E-EL-R0488

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat HO1 in samples. No significant cross-reactivity or interference between Rat HO1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat HO1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HO1. You can calculate the concentration of Rat HO1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HO1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HO1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.51 1.37 4.10 0.46 1.37 4.04
Standard deviation 0.03 0.06 0.16 0.03 0.06 0.20
CV (%) 5.88 4.38 3.90 6.52 4.38 4.95

Recovery

The recovery of Rat HO1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 83-96 90
EDTA plasma (n=8) 90-103 98
Cell culture media (n=8) 88-103 94

Linearity

Samples were spiked with high concentrations of Rat HO1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P06762
SynonymsHO1, HMOX1, HMOX1D, HSP32, Bk286b10
Research AreaCancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Antioxidants (2022) IF: 6.313
    Origanum majorana L. Extract Attenuated Benign Prostatic Hyperplasia in Rat Model: Effect on Oxidative Stress, Apoptosis, and Proliferation

    DOI: 10.3390/antiox11061149

    PMID: 35740046

    		 Sample: prostate tissue
  2. Food & Function (2022) IF: 6.317
    Serum metabolomics analysis reveals amelioration effects of sea cucumber ether phospholipids on oxidative stress and inflammation in high-fat diet-fed mice

    DOI: 10.1039/D2FO00918H

    PMID: 36106708

    		 Sample: serum
  3. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Carveol Promotes Nrf2 Contribution in Depressive Disorders through an Anti-inflammatory Mechanism

    DOI: 10.1155/2022/4509204

    PMID: 35295720

    		 Sample: cortical,hippocampal brain
  4. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 5.572
    Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway

    DOI: 10.1016/j.fct.2022.113471

    PMID: 36270409

    		 Sample: liver,kidney
  5. Frontiers in Pharmacology (2021) IF: 5.811
    Carveol a Naturally-Derived Potent and Emerging Nrf2 Activator Protects Against Acetaminophen-Induced Hepatotoxicity

    DOI: 10.3389/fphar.2020.621538

    PMID: 33597885

    		 Sample: Tissue homogenate
  6. Genes (2022) IF: 4.141
    Nrf2/HO-1 Signaling Stimulation through Acetyl-11-Keto-Beta-Boswellic Acid (AKBA) Provides Neuroprotection in Ethidium Bromide-Induced Experimental Model of Multiple Sclerosis

    DOI: 10.3390/genes13081324

    PMID: 35893061

    		 Sample: brain,CSF
  7. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.219
    Doxorubicin-induced Hepatic Toxicity in Rats: Mechanistic Protective Role of Omega-3 Fatty Acids through Nrf2/HO-1 activation and PI3K/Akt/GSK-3β Axis Modulation

    DOI: 10.1016/j.sjbs.2022.103308

    PMID: 35677895

    		 Sample: Hepatic
  8. International Journal of Nanomedicine (2017) IF: 4.3
    Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo

    DOI: 10.2147/IJN.S134897
    Sample: Cell culture supernatant,Serum
  9. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats

    DOI: 10.1016/j.ejphar.2021.174044

    PMID: 33745959

    		 Sample: Tissue homogenate(Joint Tissue)
  10. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats

    DOI: 10.1007/s10787-020-00785-5

    PMID: 33459879

    		 Sample: Tissue homogenate(hippocampus)
  11. TOXICOLOGY (2022) IF: 4.571
    Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF‑kB signaling in female albino rats

    DOI: 10.1016/j.tox.2022.153350

    PMID: 36220500

    		 Sample: liver,kidney
  12. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    Effects of sulforaphane on D-galactose-induced liver aging in rats: Role of keap-1/nrf-2 pathway.

    DOI: 10.1016/j.ejphar.2019.04.043
    Sample: liver tissue
  13. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Octreotide alleviates pancreatic damage caused by paraquat in rats by reducing inflammatory responses and oxidative stress

    DOI: 10.1016/j.etap.2020.103456

    PMID: 32673753

    		 Sample: Serum,Tissue homogenate
  14. TOXICOLOGY LETTERS (2019) IF: 3.499
    The interrelationship between gasotransmitters and lead-induced renal toxicity in rats

    DOI: 10.1016/j.toxlet.2019.04.012
    Sample: kidney tissues
  15. LIFE SCIENCES (2020) IF: 3.647
    New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signaling

    DOI: 10.1016/j.lfs.2020.117581

    PMID: 32209424

    		 Sample: Tissue homogenate
  16. Frontiers in Neuroscience (2020) IF: 3.707
    Potent Natural Antioxidant Carveol Attenuates MCAO-Stress Induced Oxidative, Neurodegeneration by Regulating the Nrf-2 Pathway

    DOI: 10.3389/fnins.2020.00659
    Sample: Tissue homogenate
  17. NEUROSCIENCE (2023) IF: 3.708
    Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate

    DOI: 10.1016/j.neuroscience.2023.01.002

    PMID: 36646412

    		 Sample: cerebral cortex,cerebellum
  18. FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775
    Activation of pCREB/Nrf-2 signaling mediates re-positioning of liraglutide as hepato-protective for methotrexate -induced liver injury (MILI)

    DOI: 10.1016/j.fct.2019.110719

    PMID: 31362085

    		 Sample: Serum
  19. TISSUE & CELL (2021) IF: 2.466
    The potential relationship between gasotransmitters and oxidative stress, inflammation and apoptosis in lead-induced hepatotoxicity in rats

    DOI: 10.1016/j.tice.2021.101511

    PMID: 33725649

    		 Sample: Tissue（liver）
  20. Journal of Plastic Surgery and Hand Surgery (2019) IF: 1.037
    Transferring the protective effect of remote ischemic preconditioning on skin flap among rats by blood serum

    DOI: 10.1080/2000656X.2019.1582422

    PMID: 30888241

    		 Sample: Serum
  21. Current Research in Toxicology (2022)
    Zn and Se abrogate heavy metal mixture induced ovarian and thyroid oxido-inflammatory effects mediated by activation of NRF2-HMOX-1 in female albino rats

    DOI: 10.1016/j.crtox.2022.100098
    Sample: ovarian,thyroid,thyroid cell
  22. Chinese Herbal Medicines (2019)
    Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats

    DOI: 10.1016/j.chmed.2018.12.004
    Sample: Plasma,Serum,Tissue homogenate
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