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Rat I-PTH(Intact Parathormone) ELISA Kit

  • Cat.No.:E-EL-R0535

  • Reactivity: Rat

To Purchase E-EL-R0535

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat I-PTH in samples. No significant cross-reactivity or interference between Rat I-PTH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat I-PTH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat I-PTH. You can calculate the concentration of Rat I-PTH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat I-PTH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat I-PTH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.45 154.88 373.68 45.17 166.01 402.93
Standard deviation 2.60 6.91 19.51 3.04 7.29 13.94
CV (%) 5.26 4.46 5.22 6.73 4.39 3.46


The recovery of Rat I-PTH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 95
EDTA plasma (n=8) 92-107 98
Cell culture media (n=8) 94-109 101


Samples were spiked with high concentrations of Rat I-PTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaSignal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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  1. LIFE SCIENCES (2022) IF: 6.78
    The Cannabinoid-2 receptor agonist, 1-phenylisatin, protects against cisplatin-induced nephrotoxicity in mice

    DOI: 10.1016/j.lfs.2022.120928

    PMID: 36058263

    Sample: kidney tissue
  2. Stem Cell Research & Therapy (2020) IF: 6.832
    Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model

    DOI: 10.1186/s13287-020-01733-4

    PMID: 32456711

    Sample: Plasma
  3. Food & Function (2022) IF: 5.396
    Diosmin, a citrus fruit-derived phlebotonic bioflavonoid protects rats from chronic kidney disease-induced loss of bone mass and strength without deteriorating the renal function

    DOI: 10.1039/D1FO03867B

    PMID: 35119062

    Sample: serum
  4. BONE (2020) IF: 4.147
    Calcium repletion to rats with calcipenic rickets fails to recover bone quality: A calcipenic “memory”

    DOI: 10.1016/j.bone.2020.115562

    Sample: serum
  5. Frontiers in Pharmacology (2020) IF: 4.225
    Bazedoxifene Attenuates Abdominal Aortic Aneurysm Formation via Downregulation of Interleukin-6/Glycoprotein 130/Signal Transducer and Activator of Transcription 3 Signaling Pathway in Apolipoprotein E–Knockout Mice

    DOI: 10.3389/fphar.2020.00392

    Sample: Cell culture medium
  6. Frontiers in Physiology (2023) IF: 4.755
    Relative comparison of chronic kidney disease-mineral and bone disorder rat models

    DOI: 10.3389/fphys.2023.1083725

    Sample: Serum
  7. Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
    Beneficial Effects of Total Phenylethanoid Glycoside Fraction Isolated from Cistanche deserticola on Bone Microstructure in Ovariectomized Rats

    DOI: 10.1155/2019/2370862

    PMID: 31346358

    Sample: Serum,Urine
  8. BIOMETALS (2022) IF: 3.378
    Iron overload impairs renal function and is associated with vascular calcification in rat aorta

    DOI: 10.1007/s10534-022-00449-7

    PMID: 36178540

    Sample: serum
  9. ENDOCRINE (2022) IF: 3.925
    Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling

    DOI: 10.1007/s12020-022-03199-1

    PMID: 36184719

    Sample: serum
  10. Annals of Translational Medicine (2021) IF: 3.932
    Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B

    DOI: 10.21037/atm-21-934

    PMID: 33850900

    Sample: Serum
  11. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Effect of parathyroid hormone on cardiac function in rats with cardiomyopathy

    DOI: 10.3892/etm.2018.6528

    PMID: 30214507

    Sample: Serum
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