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Rat IFN-γ(Interferon Gamma) ELISA Kit

Citations(14)Uniprot : P01581

Cat.No.:E-EL-R0009
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat IFN-γ in samples. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IFN-γ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	109.20	267.20	908.90	99.30	278.90	944.10
Standard deviation	6.00	14.70	48.20	6.10	16.50	34.00
CV (%)	5.49	5.50	5.30	6.14	5.92	3.60

Recovery

The recovery of Rat IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-97	91
EDTA plasma (n=8)	90-103	98
Cell culture media (n=8)	91-106	98

Linearity

Samples were spiked with high concentrations of Rat IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01581
Synonyms	IFNG, IFG, IFI, Type II Interferon
Research Area	Cancer, Immunology, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Cellular and Molecular Life Sciences (2016) IF: 5.788

Enteric Glial Cells Are Susceptible To Clostridium Difficile Toxin B.

DOI: 10.1007/s00018-016-2426-4
PMID: 27891552	Sample: Cell culture supernatant

Neuroscience (2018) IF: 3.056

Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest

DOI: 10.1016/j.neuroscience.2018.09.041
PMID: 30300703	Sample: Serum

Apoptosis (2018) IF: 3.967

miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

DOI: 10.1007/s10495-018-1474-7
PMID: 30167920	Sample: Serum

Journal of cellular physiology (2019) IF: 3.923

Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

DOI: 10.1002/jcp.27501
PMID: 30580448	Sample: Serum

Toxins (2018) IF: 3.273

The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

DOI: 10.3390/toxins10110449
PMID: 30384460	Sample: Serum, Tissue homogenate

Biomedicine & Pharmacotherapy (2018) IF: 3.457

Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

DOI: 10.1016/j.biopha.2018.07.081
PMID: 30119204	Sample: Serum

Toxicology Research (2020) IF: 2.283

The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

DOI: 10.1093/toxres/tfaa030
PMID: 32670559	Sample: Tissue homogenate

Journal of cellular biochemistry (2018) IF: 2.959

Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

DOI: 10.1002/jcb.28243
PMID: 30520122	Sample: Serum,Bronchoalveolar Lavage Fluid

American Journal of Reproductive Immunology (2019) IF: 2.745

Nisin Reduces Uterine Inflammation in Rats by Modulating Concentrations of Pro- And Anti-Inflammatory Cytokines

DOI: 10.1111/aji.13096
PMID: 30681748	Sample: Serum

Theranostics (2020) IF: 8.5790

The probable roles of valsartan in alleviating chronic obstructive pulmonary disease following co-exposure to cold stress and fine particulate matter.

DOI: 10.7150/thno.45777
	Sample: Tissue homogenate

Virus Research (2014) IF: 2.628

Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.

DOI: 10.1016/j.virusres.2014.10.011
PMID: 25451068	Sample: Tissue homogenate

Experimental and Therapeutic Medicine (2020) IF: 1.785

Macleaya cordata extracts exert antiviral effects in newborn mice with rotavirus-induced diarrhea via inhibiting the JAK2/STAT3 signaling pathway

DOI: 10.3892/etm.2020.8766
PMID: 32742353	Sample: Feces

in vivo (2019) IF: 1.541

Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

DOI: 10.21873/invivo.11624
PMID: 31471392	Sample: Bronchoalveolar Lavage Fluid

Theranostics (2020) IF: 8.5790

Reducing PD-L1 expression with an self-asembled nanodrug: an alternative to PD-L1 antibody for enhanced chemo-immunotherapy

DOI: 10.7150/thno.45777
	Sample: Tissue homogenate

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