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Rat IFN-γ(Interferon Gamma) ELISA Kit

  • Cat.No.:E-EL-R0009

  • Reactivity: Rat

To Purchase E-EL-R0009

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IFN-γ in samples. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IFN-γ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 109.20 267.20 908.90 99.30 278.90 944.10
Standard deviation 6.00 14.70 48.20 6.10 16.50 34.00
CV (%) 5.49 5.50 5.30 6.14 5.92 3.60

Recovery

The recovery of Rat IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-97 91
EDTA plasma (n=8) 90-103 98
Cell culture media (n=8) 91-106 98

Linearity

Samples were spiked with high concentrations of Rat IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01581
SynonymsIFNG, IFG, IFI, Type II Interferon
Research AreaCancer,Immunology,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cellular and Molecular Life Sciences (2016) IF: 5.788
    Enteric Glial Cells Are Susceptible To Clostridium Difficile Toxin B.

    DOI: 10.1007/s00018-016-2426-4

    PMID: 27891552

    Sample: Cell culture supernatant
  2. Neuroscience (2018) IF: 3.056
    Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest

    DOI: 10.1016/j.neuroscience.2018.09.041

    PMID: 30300703

    Sample: Serum
  3. Apoptosis (2018) IF: 3.967
    miR-A-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

    DOI: 10.1007/s10495-018-1474-7

    PMID: 30167920

    Sample: Serum
  4. Journal of cellular physiology (2019) IF: 3.923
    Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

    DOI: 10.1002/jcp.27501

    PMID: 30580448

    Sample: Serum
  5. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    Sample: Serum, Tissue homogenate
  6. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

    DOI: 10.1016/j.biopha.2018.07.081

    PMID: 30119204

    Sample: Serum
  7. Toxicology Research (2020) IF: 2.283
    The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

    DOI: 10.1093/toxres/tfaa030/5837334

    PMID: 99999999

    Sample: Tissue homogenate
  8. Toxicology Research (2020) IF: 2.283
    The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

    DOI: 10.1093/toxres/tfaa030

    PMID: 32670559

    Sample: Tissue homogenate
  9. Journal of cellular biochemistry (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    Sample: Serum,Bronchoalveolar Lavage Fluid
  10. American Journal of Reproductive Immunology (2019) IF: 2.745
    Nisin Reduces Uterine Inflammation in Rats by Modulating Concentrations of Pro- And Anti-Inflammatory Cytokines

    DOI: 10.1111/aji.13096

    PMID: 30681748

    Sample: Serum
  11. Virus Research (2014) IF: 2.628
    Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.

    DOI: 10.1016/j.virusres.2014.10.011

    PMID: 25451068

    Sample: Tissue homogenate
  12. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Macleaya cordata extracts exert antiviral effects in newborn mice with rotavirus-induced diarrhea via inhibiting the JAK2/STAT3 signaling pathway

    DOI: 10.3892/etm.2020.8766

    PMID: 32742353

    Sample: Feces
  13. in vivo (2019) IF: 1.541
    Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

    DOI: 10.21873/invivo.11624

    PMID: 31471392

    Sample: Bronchoalveolar Lavage Fluid
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