Rat IL-1β(Interleukin 1 Beta) ELISA Kit (E-EL-R0012)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the OD of the samples to the standard curve.

Interleukin-1 beta (IL-1beta) is a prototypical pro-inflammatory cytokine of the interleukin-1 family and a central mediator of host defense against infection and tissue injury. It is synthesized as the inactive precursor pro-IL-1beta, which lacks a classical secretory signal peptide, and is produced mainly by activated monocytes, macrophages and dendritic cells after stimulation through Toll-like receptors or by tumor necrosis factor and other cytokines. Secretion of mature IL-1beta requires proteolytic processing: inflammasome assembly activates caspase-1, which cleaves the precursor, and the mature cytokine exits the cell through gasdermin-D pores or other non-classical routes. Released IL-1beta engages the type I IL-1 receptor with its accessory protein, recruiting MyD88 and IRAK to activate NF-kappaB and MAPK signaling. This induces inflammatory mediators, adhesion molecules and acute-phase proteins, coordinating fever, leukocyte recruitment and systemic inflammation. The activity of IL-1beta is restrained by the interleukin-1 receptor antagonist and by soluble and decoy receptors, and failure of these controls contributes to autoinflammatory and chronic autoimmune diseases. This ELISA kit enables quantitative determination of rat IL-1beta in serum, plasma and cell culture supernatants, allowing innate immune activation and the efficacy of anti-inflammatory interventions to be quantified.