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Rat IL-18(Interleukin 18) ELISA Kit

  • Cat.No.:E-EL-R0567

  • Reactivity: Rat

To Purchase E-EL-R0567

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-18 in samples. No significant cross-reactivity or interference between Rat IL-18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-18 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-18. You can calculate the concentration of Rat IL-18 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-18 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.00 118.30 412.00 56.70 128.40 387.30
Standard deviation 3.60 4.90 21.00 3.10 6.50 12.80
CV (%) 6.92 4.14 5.10 5.47 5.06 3.30

Recovery

The recovery of Rat IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 91
EDTA plasma (n=8) 95-108 101
Cell culture media (n=8) 88-102 93

Linearity

Samples were spiked with high concentrations of Rat IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P97636
SynonymsIL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor
Research AreaCancer, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Aging (2020) IF: 4.831
    Salidroside ameliorates Parkinson's disease by inhibiting NLRP3-dependent pyroptosis

    DOI: 10.18632/aging.103215

    PMID: 32432571

    Sample: Cell culture supernatant,Tissue homogenate
  2. Laboratory Investigation (2018) IF: 4.254
    The role of Ca 2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis

    DOI: 10.1038/s41374-018-0135-3

    PMID: 30487596

    Sample: Plasma
  3. Oxidative Medicine and Cellular Longevity (2018) IF: 4.936
    Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways.

    DOI: 10.1155/2018/9647809

    PMID: 29977458

    Sample: Cells
  4. Oxidative Medicine and Cellular Longevity (2016) IF: 4.593
    Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved In The Susceptibility To Ischemic Acute Kidney Injury In Diabetes.

    DOI: 10.1155/2016/2386068

    PMID: 27867451

    Sample: Tissue homogenate
  5. Annals of Translational Medicine (2020) IF: 3.297
    Palmitic acid stimulates NLRP3 inflammasome activation through TLR4-NF-κB signal pathway in hepatic stellate cells

    DOI: 10.21037/atm.2020.02.21

    PMID: 32309315

    Sample: Serum,Cell culture supernatant,Tissue homogenate
  6. IF: 3.743
    Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma
    Sample: Serum,Tissue homogenates
  7. Biomedicine & Pharmacotherapy (2019) IF: 3.743
    Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma

    DOI: 10.1016/j.biopha.2019.109188

    PMID: 31315072

    Sample: Serum,Tissue homogenate
  8. Acta Cirurgica Brasileira (2018) IF: 3.0895
    Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation.

    DOI: 10.1590/s0102-865020180030000006

    PMID: 29668774

    Sample: Serum
  9. ACS omega (2020) IF: 2.87
    Hydroxysafflor Yellow A Together with Blood-Brain Barrier Regulator Lexiscan for Cerebral Ischemia Reperfusion Injury Treatment

    DOI: 10.1021/acsomega.0c02502

    PMID: 32775917

    Sample: Tissue homogenate
  10. Molecular and Cellular Biochemistry (2019) IF: 2.795
    The protective effect of cannabinoid type 2 receptor activation on renal ischemia–reperfusion injury

    DOI: 10.1007/s11010-019-03616-6

    PMID: 31446615

    Sample: Serum
  11. Journal of Ovarian Research (2020) IF: 2.989
    The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats

    DOI: 10.1186/s13048-020-00650-7

    PMID: 32386521

    Sample: Serum
  12. FEBS Open bio (2020) IF: 2.231
    ASIC1a inhibits cell pyroptosis induced by acid‐induced activation of rat hepatic stellate cells

    DOI: 10.1002/2211-5463.12850

    PMID: 32237041

    Sample: Cell culture medium
  13. Radiation Oncology (2020) IF: 2.817
    Hippocampal changes in inflammasomes, apoptosis, and MEMRI after radiation-induced brain injury in juvenile rats

    DOI: 10.1186/s13014-020-01525-3

    PMID: 32276638

    Sample: Tissue homogenate
  14. RSC Advances (2019) IF: 2.936
    Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation

    DOI: 10.1039/C8RA08662A

    Sample: Tissue homogenate
  15. Journal of cellular biochemistry (2019) IF: 2.959
    Protective effect of N-(p-amylcinnamoyl) anthranilic acid, phospholipase A2 enzyme inhibitor, and transient receptor potential melastatin-2 channel blocker against renal ischemia–reperfusion injury

    DOI: 10.1002/jcb.27664

    PMID: 30259992

    Sample: Serum
  16. Journal of diabetes research (2019) IF: 2.885
    Lipopolysaccharide (LPS) Aggravates High Glucose-and Hypoxia/Reoxygenation-Induced Injury through Activating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis in H9C2 Cardiomyocytes

    DOI: 10.1155/2019/8151836

    PMID: 30911553

    Sample: Cell culture supernatant
  17. BioMed Research International (2019) IF: 2.197
    Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    DOI: 10.1155/2019/3018357

    PMID: 31240210

    Sample: Cell culture supernatant
  18. Experimental and Therapeutic Medicine (2018) IF: 1.411
    Thymoquinone reduces spinal cord injury by inhibiting inflammatory response, oxidative stress and apoptosis via PPARγ and PI3K/Akt pathways.

    DOI: 10.3892/etm.2018.6072

    PMID: 29904397

    Sample: Tissue homogenate
  19. Journal of Family and Reproductive Health (2020)
    Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats

    DOI: 10.1177/1535370220931038

    PMID: 32493122

    Sample: Tissue homogenate
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