Current Position:Home>>Kits>>ELISA Kits>> Rat IL-18(Interleukin 18) ELISA Kit
Cat.No.:E-EL-R0567
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat IL-18 in samples. No significant cross-reactivity or interference between Rat IL-18 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat IL-18 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-18 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 52.00 | 118.30 | 412.00 | 56.70 | 128.40 | 387.30 |
Standard deviation | 3.60 | 4.90 | 21.00 | 3.10 | 6.50 | 12.80 |
CV (%) | 6.92 | 4.14 | 5.10 | 5.47 | 5.06 | 3.30 |
The recovery of Rat IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 85-100 | 91 |
EDTA plasma (n=8) | 95-108 | 101 |
Cell culture media (n=8) | 88-102 | 93 |
Samples were spiked with high concentrations of Rat IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P97636
|
Synonyms | IL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor |
Research Area | Cancer, Metabolism, Immunology |
![]() |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
![]() |
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
![]() |
3. Aspirate and wash the plate for 3 times |
![]() |
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
![]() |
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
![]() |
6. Add 50μL Stop Solution |
![]() |
7. Read the plate at 450nm immediately. Calculation of the results |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
Sample: serum |
DOI: 10.1155/2022/8215842 |
|
PMID: 35847592 |
Sample: Serum,H9C2 cell |
PMID: 36139384 |
Sample: PC12 cell |
Sample: serum |
Sample: brain tissue |
PMID: 33848736 |
Sample: Cell sample,Tissue Slice |
PMID: 33711550 |
Sample: Whole Blood |
PMID: 35671584 |
Sample: serum |
PMID: 34474337 |
Sample: H9c2 cell |
DOI: 10.1155/2016/2386068 |
|
Sample: Tissue homogenate |
PMID: 36302499 |
Sample: myocardium,serum |
DOI: 10.1155/2020/2963540 |
|
PMID: 33294118 |
Sample: Cell culture medium |
Sample: blood,Small intestine tissue |
Sample: serum |
PMID: 34692767 |
Sample: H9C2 cell |
PMID: 35111693 |
Sample: serum,hippocampal tissue |
PMID: 35286937 |
Sample: serum |
PMID: 35578102 |
Sample: hippocampus |
Sample: serum |
PMID: 34646128 |
Sample: penumbra tissue |
PMID: 33203418 |
Sample: Tissue homogenate |
PMID: 33633569 |
Sample: Serum |
PMID: 34610336 |
Sample: plasma, heart |
DOI: 10.1002/tox.23358 |
|
PMID: 34464015 |
Sample: serum |
DOI: 10.1002/tox.23471 |
|
PMID: 35099110 |
Sample: serum |
PMID: 30487596 |
Sample: Plasma |
DOI: 10.1002/cbin.12002 |
|
Sample: serum |
PMID: 34106384 |
Sample: serum |
PMID: 35792203 |
Sample: H9C2 cell |
Sample: BV-2 cells |
PMID: 32432571 |
Sample: Cell culture supernatant,Tissue homogenate |
PMID: 33872850 |
Sample: Bronchoalveolar Lavage Fluid |
PMID: 33752080 |
Sample: Serum,Cell culture supernatant |
PMID: 33725636 |
Sample: serum,Cell culture supernatant(THP-1 cells) |
DOI: 10.1155/2018/9647809 |
|
Sample: Cells |
DOI: 10.1155/2019/8151836 |
|
Sample: Cell culture supernatant |
DOI: 10.1039/C8RA08662A |
|
Sample: Tissue homogenate |
DOI: 10.1002/tox.22902 |
|
Sample: spinal cord tissue |
Sample: lung tissues,Bone marrow-derived macrophage (BMDMs |
Sample: brain |
PMID: 35820551 |
Sample: serum |
PMID: 32309315 |
Sample: Serum,Cell culture supernatant,Tissue homogenate |
PMID: 35856408 |
Sample: blood |
DOI: 10.3390/jpm13030377 |
|
Sample: lung tissues |
Sample: serum |
PMID: 31315072 |
Sample: Serum,Tissue homogenate |
DOI: 10.1530/REP-20-0643 |
|
PMID: 33989172 |
Sample: Ovary |
DOI: 10.21037/atm-21-6822 |
|
PMID: 35284559 |
Sample: serum |
DOI: 10.1155/2019/4921086 |
|
Sample: gastric tissue |
DOI: 10.1155/2019/3018357 |
|
PMID: 31240210 |
Sample: Cell culture supernatant |
PMID: 32237041 |
Sample: Cell culture medium |
Sample: serum |
PMID: 34650639 |
Sample: brain tissue |
Sample: serum |
DOI: 10.1155/2021/6613140 |
|
Sample: Tissue homogenate |
DOI: 10.1155/2021/8874757 |
|
PMID: 33613687 |
Sample: Serum |
Sample: Tissue homogenate |
PMID: 36277145 |
Sample: AC16 cell |
PMID: 32276638 |
Sample: Tissue homogenate |
Sample: Tissue homogenate |
PMID: 31446615 |
Sample: Serum |
PMID: 35419615 |
Sample: Normal rat hepatocytes (BRL) |
DOI: 10.1002/jcb.27664 |
|
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Tissue homogenate |
Sample: hippocampus |
Sample: serum |
DOI: 10.12659/MSM.920668 |
|
Sample: serum,intestinal tissue |
DOI: 10.12659/MSM.917213 |
|
Sample: cortical |
Sample: serum |
Sample: serum |