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Rat IL-1α(Interleukin 1 Alpha) ELISA Kit

  • Cat.No.:E-EL-R0011

  • Reactivity: Rat

To Purchase E-EL-R0011

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-1α in samples. No significant cross-reactivity or interference between Rat IL-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1α. You can calculate the concentration of Rat IL-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-1α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.60 159.90 473.30 45.40 160.00 489.20
Standard deviation 2.40 7.50 22.70 2.40 8.80 20.50
CV (%) 5.26 4.69 4.80 5.29 5.50 4.19


The recovery of Rat IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 94
EDTA plasma (n=8) 86-99 92
Cell culture media (n=8) 94-108 101


Samples were spiked with high concentrations of Rat IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P16598
SynonymsIL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha
Research AreaCancer, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Neuroinflammation (2020) IF: 5.793
    Microglia induce the transformation of A1/A2 reactive astrocytes via the CXCR7/PI3K/Akt pathway in chronic post-surgical pain

    DOI: 10.1186/s12974-020-01891-5

    PMID: 32665021

    Sample: Tissue homogenate
  2. Neurobiology of aging (2019) IF: 4.454
    CIP2A-promoted astrogliosis induces AD-like synaptic degeneration and cognitive deficits

    DOI: 10.1016/j.neurobiolaging.2018.11.023

    PMID: 30594047

    Sample: Tissue homogenate,Cell culture medium
  3. Chemosphere (2018) IF: 4.427
    Fluoride altered rat's blood testis barrier by affecting the F-actin via IL-1α.

    DOI: 10.1016/j.chemosphere.2018.08.009

    PMID: 30099167

    Sample: Tissue homogenate
  4. International Journal of Endocrinology (2015) IF: 2.51
    17β Estradiol Modulates Perfusion Pressure And Expression Of 5-LOX And CYP450 4A In The Isolated Kidney Of Metabolic Syndrome Female Rats.

    DOI: 10.1155/2015/149408

    PMID: 26491436

    Sample: Tissue homogenate
  5. International Immunopharmacology (2015) IF: 2.956
    Anti-Inflammatory Effects And Pharmacokinetics Study Of Geniposide On Rats With Adjuvant Arthritis.

    DOI: 10.1016/j.intimp.2014.11.017

    PMID: 25434608

    Sample: Cell culture supernatant
  6. Iranian Journal of Basic Medical Sciences (2018) IF: 1.424
    The Protective Effects Of Naringin Against 5-Fluorouracil-Induced Hepatotoxicity And Nephrotoxicity In Rats.

    DOI: 10.22038/IJBMS.2018.27510.6714

    PMID: 29796225

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