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Rat IL-4(Interleukin 4) ELISA Kit

  • Cat.No.:E-EL-R0014

  • Reactivity: Rat

To Purchase E-EL-R0014

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-4 in samples. No significant cross-reactivity or interference between Rat IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-4. You can calculate the concentration of Rat IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.20 134.60 394.80 47.80 134.90 433.70
Standard deviation 3.20 6.50 15.40 2.50 5.90 21.30
CV (%) 6.78 4.83 3.90 5.23 4.37 4.91

Recovery

The recovery of Rat IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma (n=8) 86-99 93
Cell culture media (n=8) 91-103 96

Linearity

Samples were spiked with high concentrations of Rat IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P20096
SynonymsIL4, BCGF-1, BCGF1, BSF-1, BSF1
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Pineal Research (2014) IF: 10.391
    Melatonin Normalizes Clinical And Biochemical Parameters Of Mild Inflammation In Diet-Induced Metabolic Syndrome In Rats.

    DOI: 10.1111/jpi.12168

    PMID: 25113124

    Sample: Plasma
  2. European review for medical and pharmacological sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (miR-2) and miR-101 on sepsis-induced myocardial injury in rats

    DOI: 10.26355/eurrev_202006_21528

    PMID: 99999999

    Sample: Serum
  3. Mediators of Inflammation (2020) IF: 3.758
    Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD

    DOI: 10.1155/2020/4260204

    PMID: 32454790

    Sample: Tissue homogenate,Bronchoalveolar Lavage Fluid
  4. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    Sample: Serum, Tissue homogenate
  5. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

    DOI: 10.1016/j.biopha.2018.07.081

    PMID: 30119204

    Sample: Serum
  6. Phytotherapy Research (2017) IF: 3.092
    Antiinflammation Effects And Mechanisms Study Of Geniposide On Rats With Collagen-Induced Arthritis.

    DOI: 10.1002/ptr.5775

    PMID: 28127805

    Sample: Cell culture supernatant
  7. Journal of cellular biochemistry (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    Sample: Serum,Bronchoalveolar Lavage Fluid
  8. Gene (2015) IF: 2.498
    study some immunological parameters in rat infected with Cryptococcus neoformans

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  9. American Journal of Reproductive Immunology (2019) IF: 2.745
    Nisin Reduces Uterine Inflammation in Rats by Modulating Concentrations of Pro- And Anti-Inflammatory Cytokines

    DOI: 10.1111/aji.13096

    PMID: 30681748

    Sample: Serum
  10. International Journal of Endocrinology (2015) IF: 2.51
    17β Estradiol Modulates Perfusion Pressure And Expression Of 5-LOX And CYP450 4A In The Isolated Kidney Of Metabolic Syndrome Female Rats.

    DOI: 10.1155/2015/149408

    PMID: 26491436

    Sample: Tissue homogenate
  11. International Immunopharmacology (2014) IF: 2.956
    Effects And Mechanisms Of Geniposide On Rats With Adjuvant Arthritis.

    DOI: 10.1016/j.intimp.2014.02.021

    PMID: 24583144

    Sample: Cell lysate
  12. in vivo (2019) IF: 1.541
    Oral Administration of Chitosan Attenuates Bleomycin-induced Pulmonary Fibrosis in Rats

    DOI: 10.21873/invivo.11624

    PMID: 31471392

    Sample: Bronchoalveolar Lavage Fluid
  13. Chemical and Pharmaceutical Bulletin (2018) IF: 1.133
    Effect of the Tibetan Medicine Zuotai on Degranulation and Inflammatory Mediator Release in RBL-2H3 Cells.

    DOI: 10.1248/cpb.c18-00014

    PMID: 29848888

    Sample: Cells
  14. Toxicology Research (2017) IF: 1.969
    Nano NIO Induced Liver Toxicity Via Activating The NF-κB Signaling Pathway In Rats.

    DOI: 10.1039/c6tx00444j

    PMID: 30090495

    Sample: Tissue homogenate
  15. Preprint (2020)
    Characterization of Essential Fatty Acid Semicarbazide (EFASC) from Linum usitatissium as a Safe and Effective Adjuvant

    DOI: 99999999

    PMID: 99999999

    Sample: Serum
  16. Biochem. Cell. Arch. (2018) IF: /
    EVALUATE THE IMMUNE RESPONSE OF ACINETOBACTER BAUMANNII ANTIGENS IN WHITE ALBION RATS.
    Sample: Serum
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