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Rat KIM-1(Kidney Injury Molecule 1) ELISA Kit

Citations(23)

  • Cat.No.:E-EL-R3019

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat KIM-1 in samples. No significant cross-reactivity or interference between Rat KIM-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat KIM-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat KIM-1. You can calculate the concentration of Rat KIM-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat KIM-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat KIM-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.92 2.30 8.98 0.97 2.38 9.60
Standard deviation 0.05 0.11 0.49 0.05 0.11 0.45
CV (%) 5.43 4.78 5.46 5.15 4.62 4.69

Recovery

The recovery of Rat KIM-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 92
EDTA plasma (n=8) 87-101 95
Cell culture media (n=8) 90-101 94

Linearity

Samples were spiked with high concentrations of Rat KIM-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsHAVCR1, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1
Research AreaCancer, Immunology, Microbiology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400
    Sample: plasma
  2. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Renoprotective and therapeutic effects of newly water, ethanol, and butanol ginseng fractions in hypertensive and chronic kidney disease with L-NAME

    DOI: 10.1016/j.biopha.2021.111978

    PMID: 34411920

    		 Sample: serum
  3. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Preventing the onset of diabetes-induced chronic kidney disease during prediabetes: The effects of oleanolic acid on selected markers of chronic kidney disease in a diet-induced prediabetic rat model

    DOI: 10.1016/j.biopha.2021.111570

    PMID: 33932738

    		 Sample: Plasma
  4. LIFE SCIENCES (2021) IF: 5.037
    Epigenetic restoration of endogenous Klotho expression alleviates acute kidney injury-diabetes comorbidity

    DOI: 10.1016/j.lfs.2021.120194

    PMID: 34864061

    		 Sample: urinary
  5. LIFE SCIENCES (2022) IF: 5.037
    Angiotensin 1–7 mitigates rhabdomyolysis induced renal injury in rats via modulation of TLR-4/NF-kB/iNOS and Nrf-2/heme‑oxygenase-1 signaling pathways

    DOI: 10.1016/j.lfs.2022.120678
    Sample: serum
  6. LIFE SCIENCES (2021) IF: 5.037
    Agomelatine prevents gentamicin nephrotoxicity by attenuating oxidative stress and TLR-4 signaling, and upregulating PPARγ and SIRT1

    DOI: 10.1016/j.lfs.2021.119600

    PMID: 33984362

    		 Sample: Serum
  7. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Ameliorative Effects of Bredemolic Acid on Markers Associated with Renal Dysfunction in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2978340

    PMID: 32655765

    		 Sample: Plasma,Urine,Tissue homogenate
  8. FASEB JOURNAL (2020) IF: 5.192
    Pre‐ischemic renal lavage protects against renal ischemia‐reperfusion injury by attenuation of local and systemic inflammatory responses

    DOI: 10.1096/fj.201902943R
    Sample: Urine
  9. PHYTOMEDICINE (2022) IF: 5.34
    Piperazine ferulate attenuates gentamicin-induced acute kidney injury via the NF-κB/NLRP3 pathway

    DOI: 10.1016/j.phymed.2022.154021

    PMID: 35286937

    		 Sample: serum
  10. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    Saroglitazar, a dual PPAR-α/γ agonist, alleviates LPS-induced hepatic and renal injury in rats

    DOI: 10.1016/j.intimp.2023.109688
    Sample: liver
  11. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Protective effect of fatty acid amide hydrolase inhibitor URB597 and monoacylglycerol lipase inhibitor KML29 on renal ischemia–reperfusion injury via toll-like receptor 4/nuclear factor-kappa B pathway

    DOI: 10.1016/j.intimp.2022.109586
    Sample: serum
  12. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Amelioration of risk factors associated with diabetic nephropathy in diet-induced pre-diabetic rats by an uracil-derived diimine ruthenium(II) compound

    DOI: 10.1016/j.biopha.2020.110483

    PMID: 32768965

    		 Sample: Plasma,Tissue homogenate
  13. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    Umbelliferone attenuates gentamicin-induced renal toxicity by suppression of TLR-4/NF-κB-p65/NLRP-3 and JAK1/STAT-3 signaling pathways

    DOI: 10.1007/s11356-020-11416-5

    PMID: 33128149

    		 Sample: Serum
  14. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    Aged garlic extract rescues ethephon-induced kidney damage by modulating oxidative stress, apoptosis, inflammation, and histopathological changes in rats

    DOI: 10.1007/s11356-020-10997-5

    PMID: 33011947

    		 Sample: Serum
  15. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.568
    An in vivo and in vitro model on the protective effect of cilnidipine on contrast-induced nephropathy via regulation of apoptosis and CaMKⅡ/mPTP pathway

    DOI: 10.1002/jbt.23238

    PMID: 36207783

    		 Sample: serum
  16. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 3.738
    Candesartan Protects Against Cadmium-Induced Hepatorenal Syndrome by Affecting Nrf2, NF-κB, Bax/Bcl-2/Cyt-C, and Ang II/Ang 1–7 Signals

    DOI: 10.1007/s12011-022-03286-4
    Sample: kidney
  17. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 3.738
    Acetaminophen-Induced Nephrotoxicity: Suppression of Apoptosis and Endoplasmic Reticulum Stress Using Boric Acid

    DOI: 10.1007/s12011-022-03114-9

    PMID: 35020164

    		 Sample: serum
  18. JOURNAL OF SURGICAL RESEARCH (2018) IF: 2.051
    Effect of Hydrogen Sulfide on Kidney Injury in Rat Model of Crush Syndrome

    DOI: 10.1016/j.jss.2018.10.033

    PMID: 30691831

    		 Sample: Tissue homogenate
  19. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2021) IF: 2.273
    Anti-hyperglycaemic effects of dioxidovanadium complex cis-[VO2(obz)py] avert kidney dysfunction in streptozotocin-induced diabetic male Sprague–Dawley rats

    DOI: 10.1139/cjpp-2020-0278
    Sample: plasma
  20. MOLECULAR AND CELLULAR BIOCHEMISTRY (2019) IF: 2.884
    The protective effect of cannabinoid type 2 receptor activation on renal ischemia–reperfusion injury

    DOI: 10.1007/s11010-019-03616-6

    PMID: 31446615

    		 Sample: Serum
  21. JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 2.959
    Protective effect of N‐(p‐amylcinnamoyl) anthranilic acid, phospholipase A2 enzyme inhibitor, and transient receptor potential melastatin‐2 channel blocker against renal ischemia–reperfusion injury

    DOI: 10.1002/jcb.27664
    Sample: Serum
  22. INTERNATIONAL UROLOGY AND NEPHROLOGY (2019) IF: 1.596
    Effects of hydrogen sulfide on acetaminophen-induced acute renal toxicity in rats

    DOI: 10.1007/s11255-018-2053-0

    PMID: 30604234

    		 Sample: Tissue homogenate
  23. European Journal of Trauma and Emergency Surgery (2019) IF: 1.781
    Beneficial effects of bardoxolone methyl, an Nrf2 activator, on crush-related acute kidney injury in rats

    DOI: 10.1007/s00068-019-01216-z

    PMID: 31471671

    		 Sample: Tissue homogenate
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