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Rat LEP(Leptin) ELISA Kit

Citations(24)Uniprot : P50596

  • Cat.No.:E-EL-R0582

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat LEP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat LEP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat LEP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.50 4.40 0.50 1.50 4.80
Standard deviation 0.03 0.08 0.17 0.03 0.08 0.23
CV (%) 6.00 5.33 3.86 6.00 5.33 4.79

Recovery

The recovery of Rat LEP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-107 101
EDTA plasma (n=8) 85-98 91
Cell culture media (n=8) 95-109 102

Linearity

Samples were spiked with high concentrations of Rat LEP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P50596
SynonymsLEPD, OB, OBS, Obesity Homolog
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance

    DOI: 10.1016/j.etap.2020.103506

    PMID: 33002592

    		 Sample: Serum
  2. Applied Physiology, Nutrition, and Metabolism (2018) IF: 3.455
    Switching to normal diet reverses kwashiorkor-induced salivary impairments via increased nitric oxide level and expression of aquaporin 5 in the submandibular glands of male Wistar rats

    DOI: 10.1139/apnm-2018-0282

    PMID: 30212637

    		 Sample: saliva
  3. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  4. Andrology (2020) IF: 2.86
    Orlistat attenuates obesity-induced decline in steroidogenesis and spermatogenesis by up-regulating steroidogenic genes

    DOI: 10.1111/andr.12824

    PMID: 32438512

    		 Sample: Tissue homogenate
  5. Journal of Diabetes Research (2015) IF: 2.717
    Edible Bird'S Nest Prevents High Fat Diet-Induced Insulin Resistance In Rats.

    DOI: 10.1155/2015/760535

    PMID: 26273674

    		 Sample: Serum
  6. Food & Nutrition Research (2016) IF: 2.039
    Perinatal Exposure To Germinated Brown Rice And Its Gamma Amino-Butyric Acid-Rich Extract Prevents High Fat Diet-Induced Insulin Resistance In First Generation Rat Offspring.

    DOI: 10.3402/fnr.v60.30209

    PMID: 26842399

    		 Sample: Serum
  7. Food & Nutrition Research (2015) IF: 2.039
    N-Acetylneuraminic Acid Attenuates Hypercoagulation On High Fat Diet-Induced Hyperlipidemic Rats.

    DOI: 10.3402/fnr.v59.29046

    PMID: 26642300

    		 Sample: Serum
  8. BioMed Research International (2015) IF: 2.476
    N-Acetylneuraminic Acid Supplementation Prevents High Fat Diet-Induced Insulin Resistance In Rats Through Transcriptional And Nontranscriptional Mechanisms.

    DOI: 10.1155/2015/602313

    PMID: 26688813

    		 Sample: Serum
  9. Journal of Developmental Origins Of Health And Disease (2017) IF: 2.07
    Effect Of Neonatal Orally Administered S-allyl Cysteine In High-Fructose Diet Fed Wistar Rats.

    DOI: 10.1017/S2040174417000940

    PMID: 29151399

    		 Sample: Plasma
  10. Islets (2017) IF: 2.2
    The Adverse Effects Of Chronic Low-Dose Exposure To Nonylphenol On Type 2 Diabetes Mellitus In High Sucrose-High Fat Diet-Treated Rats.

    DOI: 10.1080/19382014.2017.1404211

    PMID: 29215945

    		 Sample: Plasma
  11. Current Drug Delivery (2016) IF: 2.516
    Effect Of Glycine On Adipocyte Hypertrophy In A Metabolic Syndrome Rat Model.

    DOI: 10.2174/156720181301160314151554

    PMID: 26996629

    		 Sample: Serum
  12. BMC Complementary and Alternative Medicine (2017) IF: 2.288
    In Utero Exposure To Germinated Brown Rice And Its Oryzanol-Rich Extract Attenuated High Fat Diet-Induced Insulin Resistance In F1 Generation Of Rats.

    DOI: 10.1186/s12906-017-1571-0

    PMID: 28109299

    		 Sample: Serum
  13. Food & Nutrition Research (2015) IF: 2.039
    N-Acetylneuraminic Acid Attenuates Hypercoagulation On High Fat Diet-Induced Hyperlipidemic Rats.

    DOI: 10.3402/fnr.v59.29046

    PMID: 26642300

    		 Sample: Serum
  14. Drug Design (2015) IF: 2.822
    Edible Bird’S Nest Attenuates Procoagulation Effects Of High-Fat Diet In Rats.

    DOI: 10.2147/DDDT.S87772

    PMID: 26251574

    		 Sample: Serum
  15. Journal of Assisted Reproduction & Genetics (2015) IF: 2.163
    Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.

    DOI: 10.1007/s10815-015-0506-2

    PMID: 26081124

    		 Sample: Serum
  16. Canadian Journal of Physiology and Pharmacology (2018) IF: 1.822
    Combined Treatment with Interlukin-1 and Tumor Necrosis Factor-Alpha Antagonists Improve Type 2 Diabetes in Rats.

    DOI: 10.1139/cjpp-2017-0769
  17. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    		 Sample: Serum
  18. Molecular Medicine Reports (2016) IF: 1.692
    Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.

    DOI: 10.3892/mmr.2016.5712

    PMID: 27600729

    		 Sample: Serum
  19. Eastern Anatolian Journal of Science (2020)
    Peripheral Adropin Application Regulates Nutritional Behavior and Fat Tissue-mediated Energy Metabolism
    Sample: Serum,Tissue homogenate
  20. The Journal of Nutritional Biochemistry (2020)
    Fish oil and corn oil induced differential effect on beiging of visceral and subcutaneous white adipose tissue in high fat diet induced obesity

    DOI: 10.1016/j.jnutbio.2020.108458
    Sample: Serum
  21. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE (2019) IF: 0.166
    Inhibition of leptinleptinr-kisspeptinkiss1r signaling pathway promotes follicular development in polycystic ovarian rats
    Sample: Serum
  22. United States Patent Application Publication (2018)
    Compounds, Compositions and Methods for Prevention or Treatment of Liver and Lipid-Related Conditions
    Sample: Plasma
  23. Preventive nutrition and food science (2018) IF: /
    Insulinotropic Effect of S-Allyl Cysteine in Rat Pups.

    DOI: 10.3746/pnf.2018.23.1.15

    PMID: 29662843

    		 Sample: Plasma
  24. Asian Pacific Journal of Tropical Medicine (2016) IF: 0.925
    Changes Of Adipocytokine Expression After Diabetic Rats Received Sitagliptin And The Molecular Mechanism .

    DOI: 10.1016/j.apjtm.2016.07.014

    PMID: 27633305

    		 Sample: Serum
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