Current Position:Home>>Kits>>ELISA Kits>> Rat MMP-3(Matrix Metalloproteinase 3) ELISA Kit

Rat MMP-3(Matrix Metalloproteinase 3) ELISA Kit

Cat.No.:E-EL-R0619
Reactivity: Rat

Filter:

ELISA

To Purchase E-EL-R0619

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat MMP-3 in samples. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat MMP-3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-3. You can calculate the concentration of Rat MMP-3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.10	4.30	0.50	1.10	4.40
Standard deviation	0.03	0.06	0.19	0.03	0.06	0.15
CV (%)	6.00	5.45	4.42	6.00	5.45	3.41

Recovery

The recovery of Rat MMP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-110	102
EDTA plasma (n=8)	85-96	90
Cell culture media (n=8)	85-98	92

Linearity

Samples were spiked with high concentrations of Rat MMP-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	MMP3, CHDS6, SL-1, STMY, STMY1, STR1, Progelatinase
Research Area	Cancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats

DOI: 10.1016/j.biopha.2022.112948

PMID: 35430394
Sample: skin tissue
Bone & Joint Research (2021) IF: 5.853
PARP-1 inhibition attenuates the inflammatory response in the cartilage of a rat model of osteoarthritis

DOI: 10.1302/2046-3758.107.BJR-2020-0200.R2

PMID: 34254815
Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Role of Glutamine-Glutamate/GABA cycle and potential target GLUD2 in alleviation of rheumatoid arthritis by Tripterygium hypoglaucum (levl.) Hutch based on metabolomics and molecular pharmacology

DOI: 10.1016/j.jep.2021.114561

PMID: 34454056
Sample: serum
Scientific Reports (2022) IF: 4.38
Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats

DOI: 10.1038/s41598-022-09380-w

PMID: 35354886
Sample: plasma
PHYTOTHERAPY RESEARCH (2017) IF: 3.092
Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

DOI: 10.1002/ptr.5949

Sample: Serum
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model

DOI: 10.1007/s10787-018-0452-6

Sample: serum
INTERNATIONAL ENDODONTIC JOURNAL (2019) IF: 3.331
Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

DOI: 10.1111/iej.13121

Sample: Serum
Biomed Research International (2022) IF: 3.411
Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

DOI: 10.1155/2022/4117520

PMID: 35509713
Sample: plasma
International Journal of Medical Sciences (2021) IF: 3.738
Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs, NF-κB signaling pathway

DOI: 10.7150/ijms.52330

PMID: 33456343
Sample: serum
PHYTOTHERAPY RESEARCH (2019) IF: 3.766
Vernonia amygdalina inhibited osteoarthritis development by anti‐inflammatory and anticollagenase pathways in cartilage explant and osteoarthritis‐induced rat model

DOI: 10.1002/ptr.6366

Sample: serum
Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

DOI: 10.22038/ijbms.2020.45193.10527

PMID: 33149866
Sample: Tissue homogenate
JOURNAL OF FOOD BIOCHEMISTRY (2019) IF: 1.358
Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti‐inflammatory, anti‐oxidative, and anti‐protease pathways

DOI: 10.1111/jfbc.12755

Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha