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Rat MMP-9(Matrix Metalloproteinase 9) ELISA Kit

Citations(23)

  • Cat.No.:E-EL-R3021

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 ng/mL
Sensitivity 4.69 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat MMP-9 in samples. No significant cross-reactivity or interference between Rat MMP-9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat MMP-9 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-9. You can calculate the concentration of Rat MMP-9 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-9 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-9 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 23.64 61.14 223.70 24.02 56.48 214.26
Standard deviation 1.41 2.78 9.91 1.21 2.80 11.48
CV (%) 5.96 4.55 4.43 5.04 4.96 5.36

Recovery

The recovery of Rat MMP-9 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-104 101
EDTA plasma (n=8) 95-109 103
Cell culture media (n=8) 91-101 95

Linearity

Samples were spiked with high concentrations of Rat MMP-9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsMMP9, CLG4B,Gelatinase B, GELB, MANDP2, 92kDa Type IV Collagenase, 92 KDa Gelatinase
Research AreaCancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Antioxidants (2022) IF: 6.313
    Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity

    DOI: 10.3390/antiox11020412

    PMID: 35204293

    		 Sample: serum
  2. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway

    DOI: 10.1016/j.biopha.2020.111184

    PMID: 33418305

    		 Sample: Plasma
  3. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Huangbai liniment and berberine promoted wound healing in high-fat diet/Streptozotocin-induced diabetic rats

    DOI: 10.1016/j.biopha.2022.112948

    PMID: 35430394

    		 Sample: skin tissue
  4. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need

    DOI: 10.1016/j.biopha.2022.112723

    PMID: 35202914

    		 Sample: lung
  5. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition

    DOI: 10.1155/2021/8836355
    Sample: serum
  6. PHYTOMEDICINE (2022) IF: 6.656
    Intragastric administration of Pien Tze Huang enhanced wound healing in diabetes by inhibiting inflammation and improving energy generation

    DOI: 10.1016/j.phymed.2022.154578
    Sample: skin tissue
  7. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953
    Metabolites of gut microbiota fermenting Poria cocos polysaccharide alleviates chronic nonbacterial prostatitis in rats

    DOI: 10.1016/j.ijbiomac.2022.04.029

    PMID: 35398386

    		 Sample: prostate
  8. BIOMACROMOLECULES (2021) IF: 6.988
    Fabrication of In Situ Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model

    DOI: 10.1021/acs.biomac.0c01709

    PMID: 33899465

    		 Sample: Tissue homogenate,Serum
  9. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY (2022) IF: 5.067
    Niacin mitigates blood–brain barrier tight junctional proteins dysregulation and cerebral inflammation in ketamine rat model of psychosis: Role of GPR109A receptor

    DOI: 10.1016/j.pnpbp.2022.110583
    Sample: Brain tissue
  10. JOURNAL OF HEART AND LUNG TRANSPLANTATION (2014) IF: 5.611
    Effect of chronic left ventricular unloading on myocardial remodeling: Multimodal assessment of two heterotopic heart transplantation techniques

    DOI: 10.1016/j.healun.2014.11.015
    Sample: Tissue homogenate
  11. Frontiers in Pharmacology (2021) IF: 5.811
    The Mechanisms of Cucurbitacin E as a Neuroprotective and Memory-Enhancing Agent in a Cerebral Hypoperfusion Rat Model: Attenuation of Oxidative Stress, Inflammation, and Excitotoxicity.

    DOI: 10.3389/fphar.2021.794933
    Sample: brain
  12. Frontiers in Pharmacology (2021) IF: 5.811
    l-Borneol Exerted the Neuroprotective Effect by Promoting Angiogenesis Coupled With Neurogenesis via Ang1-VEGF-BDNF Pathway

    DOI: 10.3389/fphar.2021.641894

    PMID: 33746762

    		 Sample: Serum
  13. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    HGF and TSG-6 Released by Mesenchymal Stem Cells Attenuate Colon Radiation-Induced Fibrosis

    DOI: 10.3390/ijms22041790

    PMID: 33670243

    		 Sample: Tissue homogenate
  14. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Network pharmacology analysis uncovers the effect on apoptotic pathway by Bu-Fei formula for COPD treatment

    DOI: 10.1016/j.jep.2022.115022

    PMID: 35074456

    		 Sample: serum
  15. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Integrated serum pharmacochemistry and network pharmacological analysis used to explore possible anti-rheumatoid arthritis mechanisms of the Shentong-Zhuyu decoction

    DOI: 10.1016/j.jep.2021.113988

    PMID: 33667569

    		 Sample: Serum
  16. MEDIATORS OF INFLAMMATION (2020) IF: 4.711
    Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD

    DOI: 10.1155/2020/4260204

    PMID: 32454790

    		 Sample: Tissue homogenate,Bronchoalveolar Lavage Fluid
  17. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019) IF: 3.091
    Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

    DOI: 10.1111/aji.13096
    Sample: Serum
  18. PLoS One (2021) IF: 3.24
    Matrix metalloproteinase 9 a potential major player connecting atherosclerosis and osteoporosis in high fat diet fed rats

    DOI: 10.1371/journal.pone.0244650

    PMID: 33571214

    		 Sample: Tissue homogenate
  19. International Journal of Medical Sciences (2021) IF: 3.738
    Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs, NF-κB signaling pathway

    DOI: 10.7150/ijms.52330

    PMID: 33456343

    		 Sample: serum
  20. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849
    Design, in vitro bioactivity and in vivo influence on oxidative stress and matrix metalloproteinases of bioglasses in experimental skin wound

    DOI: 10.1016/j.jtemb.2021.126846

    PMID: 34438314

    		 Sample: skin
  21. SCANDINAVIAN CARDIOVASCULAR JOURNAL (2020) IF: 1.084
    St. Thomas and del Nido cardioplegia are superior to Custodiol cardioplegia in a rat model of donor heart

    DOI: 10.1080/14017431.2020.1846772

    PMID: 33185130

    		 Sample: Tissue homogenate
  22. Journal of Stroke & Cerebrovascular Diseases (2015) IF: 1.669
    Neuroprotective Effects of Brain-Derived Neurotrophic Factor and Noggin-Modified Bone Mesenchymal Stem Cells in Focal Cerebral Ischemia in Rats

    DOI: 10.1016/j.jstrokecerebrovasdis.2015.10.013
    Sample: Cell culture supernatant
  23. LaboratoriumsMedizin-Journal of Laboratory Medicine (2018) IF: 0.216
    The antiiflammatory effect of Ginkgo biloba in lipopolysaccharide-induced rat sepsis model

    DOI: 10.1515/labmed-2017-0113
    Sample: Serum
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