Current Position:Home>>Kits>>ELISA Kits>> Rat NFKB-p65(Nuclear Factor Kappa B p65) ELISA Kit
Cat.No.:E-EL-R0674
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 78.13-5000 pg/mL |
Sensitivity | 46.88 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat NFKB-p65 in samples. No significant cross-reactivity or interference between Rat NFKB-p65 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p65 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NFKB-p65 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NFKB-p65 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 247.16 | 793.15 | 2350.95 | 248.82 | 741.40 | 2123.84 |
Standard deviation | 13.77 | 35.06 | 123.19 | 12.69 | 32.03 | 93.02 |
CV (%) | 5.57 | 4.42 | 5.24 | 5.10 | 4.32 | 4.38 |
The recovery of Rat NFKB-p65 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 93-105 | 98 |
EDTA plasma (n=8) | 88-99 | 93 |
Cell culture media (n=8) | 95-111 | 102 |
Samples were spiked with high concentrations of Rat NFKB-p65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | NFKB3, p65, RelA |
Research Area | Cancer, Cell Biology, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Neuroscience, Signal transduction |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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PMID: 33711550 |
Sample: Whole Blood |
PMID: 35658227 |
Sample: testicular tissue |
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Sample: testicular tissue |
Sample: Tissue homogenate |
DOI: 10.3390/nu14194177 |
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PMID: 36235829 |
Sample: plasma |
Sample: kidney |
PMID: 36115582 |
Sample: gastric tissue |
PMID: 35963297 |
Sample: liver |
PMID: 35452635 |
Sample: blood,cardiac tissue |
Sample: Tissue homogenate |
Sample: prostatic tissue |
DOI: 10.3390/ph15111402 |
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Sample: testes |
DOI: 10.3390/ph15070852 |
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PMID: 35890148 |
Sample: testicular tissue |
PMID: 35092748 |
Sample: hippocampus,PFC,striatum tissue |
DOI: 10.1039/D1FO00802A |
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PMID: 34369542 |
Sample: liver |
DOI: 10.1039/D1FO04205J |
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Sample: adipose tissue |
PMID: 35888772 |
Sample: liver |
PMID: 35578102 |
Sample: hippocampus |
PMID: 36269541 |
Sample: PFC,hippocampal tissues |
PMID: 36059979 |
Sample: stomach tissue |
Sample: Stomach tissue |
Sample: hippocampal |
DOI: 10.1155/2022/1193392 |
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Sample: serum |
PMID: 33384599 |
Sample: Serum |
Sample: striata |
Sample: liver tissue |
PMID: 35963490 |
Sample: brain |
PMID: 34455773 |
Sample: brain tissue |
PMID: 33011199 |
Sample: Tissue homogenate |
PMID: 33745959 |
Sample: Tissue homogenate(Joint Tissue) |
PMID: 34448020 |
Sample: brain |
Sample: brain tissue |
Sample: Tissue homogenate |
Sample: skeletal muscle |
PMID: 36144661 |
Sample: stomach tissue |
PMID: 36014311 |
Sample: Stomach tissue |
PMID: 35504204 |
Sample: Peritoneal tissue |
PMID: 34953450 |
Sample: renal tissue |
Sample: Tissue homogenate |
PMID: 33011947 |
Sample: Serum |
DOI: 10.3390/life13020388 |
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Sample: gastric tissue |
PMID: 36071485 |
Sample: adipose tissue |
Sample: hippocampal |
PMID: 31767546 |
Sample: Tissue homogenate |
Sample: plasma,renal tissues |
Sample: liver tissue |
PMID: 30716985 |
Sample: Tissue homogenate |
PMID: 34228267 |
Sample: brain |
Sample: Blood |
Sample: serum and liver |
DOI: 10.3390/life12030355 |
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Sample: Heart |
DOI: 10.1042/BSR20191252 |
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PMID: 31651935 |
Sample: Serum,Plasma,Cell sample |
PMID: 32383030 |
Sample: Serum |
DOI: 10.1002/brb3.1801 |
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Sample: hippocampal tissue |
DOI: 10.1155/2022/8202975 |
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Sample: hippocampal tissue |
Sample: PC12 cells |
Sample: Plasma |
Sample: Tissue homogenate |
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Sample: Stomach tissue |
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Sample: oral mucosa |
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Sample: serum |
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