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Rat NFKB-p65(Nuclear Factor Kappa B p65) ELISA Kit

  • Cat.No.:E-EL-R0674

  • Reactivity: Rat

To Purchase E-EL-R0674

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 78.13-5000 pg/mL
Sensitivity 46.88 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NFKB-p65 in samples. No significant cross-reactivity or interference between Rat NFKB-p65 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p65 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p65. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p65 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p65, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFKB-p65. You can calculate the concentration of Rat NFKB-p65 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NFKB-p65 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NFKB-p65 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 247.16 793.15 2350.95 248.82 741.40 2123.84
Standard deviation 13.77 35.06 123.19 12.69 32.03 93.02
CV (%) 5.57 4.42 5.24 5.10 4.32 4.38

Recovery

The recovery of Rat NFKB-p65 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-105 98
EDTA plasma (n=8) 88-99 93
Cell culture media (n=8) 95-111 102

Linearity

Samples were spiked with high concentrations of Rat NFKB-p65 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

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 Browse all ancillary reagents for ELISA kits


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Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Brain, Behavior, and Immunity (2020) IF: 6.633
    Protective effect of Indole-3-carbinol, an NF-κB inhibitor in experimental paradigm of Parkinson's disease: In silico and in vivo studies

    DOI: 10.1016/j.bbi.2020.08.001

    PMID: 32800927

    Sample: Tissue homogenate
  2. Molecular Neurobiology (2018) IF: 6.19
    Stimulation of ACE2/ANG (1–7)/Mas Axis by Diminazene Ameliorates Alzheimer’s Disease in the D-Galactose-Ovariectomized Rat Model: Role of PI3K/Akt Pathway.

    DOI: 10.1007/s12035-018-0966-3

    PMID: 29516284

  3. Neuropharmacology (2020) IF: 4.431
    Caffeic acid phenethyl ester counteracts doxorubicin-induced chemobrain in Sprague-Dawley rats: Emphasis on the modulation of oxidative stress and neuroinflammation

    DOI: 10.1016/j.neuropharm.2020.108334

    PMID: 33011199

    Sample: Tissue homogenate
  4. Biochemical Pharmacology (2020) IF: 4.96
    Correlation between angiotensin 1-7-mediated Mas receptor expression with motor improvement, activated STAT3/SOCS3 cascade, and suppressed HMGB-1/RAGE/NF-κB signaling in 6-hydroxydopamine hemiparkinsonian rats

    DOI: 10.1016/j.bcp.2019.113681

    PMID: 31669235

    Sample: Tissue homogenate
  5. Environmental Science and Pollution Research (2020) IF: 3.056
    Aged garlic extract rescues ethephon-induced kidney damage by modulating oxidative stress, apoptosis, inflammation, and histopathological changes in rats

    DOI: 10.1007/s11356-020-10997-5

    PMID: 33011947

    Sample: Serum
  6. International Immunopharmacology (2020) IF: 3.943
    The ameliorating effect of cannabinoid type 2 receptor activation on brain, lung, liver and heart damage in cecal ligation and puncture-induced sepsis model in rats

    DOI: 10.1016/j.intimp.2019.105978

    PMID: 31767546

    Sample: Tissue homogenate
  7. Food Research International (2019) IF: 3.52
    Effects of chia (Salvia hispanica L.) on calcium bioavailability and inflammation in Wistar rats

    DOI: 10.1016/j.foodres.2018.08.078

    PMID: 30716985

    Sample: Tissue homogenate
  8. European Journal of pharmacology (2018) IF: 3.04
    role of Wnt4/β-catenin, Ang Ii/tgfβ, Ace2, Nf-κb, and Il-18 in attenuating renal ischemia/reperfusion-induced injury in rats treated with Vit D and pioglitazone.

    DOI: 10.1016/j.ejphar.2018.04.032

    PMID: 29715453

    Sample: Tissue homogenate
  9. Bioscience reports (2019) IF: 2.942
    Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

    DOI: 10.1042/BSR20191252

    PMID: 31651935

    Sample: Serum,Plasma,Cell sample
  10. BMC complementary and alternative medicine (2019) IF: 2.833
    The effects of Pandanus amaryllifolius (Roxb.) leaf water extracts on fructose-induced metabolic syndrome rat model

    DOI: 10.1186/s12906-019-2627-0

    PMID: 31462242

    Sample: Plasma
  11. Biomedicine & Pharmacotherapy (2017) IF: 2.759
    Cytokine Production In Vitro And In Rat Model Of Colitis In Response To Lactobacillus Plantarum LS/07.

    DOI: 10.1016/j.biopha.2017.07.138

    PMID: 28830068

    Sample: Tissue homogenate
  12. Inflammopharmacology (2017) IF: 2.59
    Ameliorative Potential Of Rutin In Combination With Nimesulide In STZ Model Of Diabetic Neuropathy: Targeting Nrf2/HO-1/NF-kB And COX Signalling Pathway.

    DOI: 10.1007/s10787-017-0413-5

    PMID: 29094308

    Sample: Tissue homogenate
  13. Journal of Ethnopharmacology (2017) IF: 2.981
    Renoprotective And Antioxidative Effects Of Methanolic Paederia Foetida Leaf Extract On Experimental Diabetic Nephropathy In Rats.

    DOI: 10.1016/j.jep.2017.01.035

    PMID: 28111217

    Sample: Serum,Tissue homogenate
  14. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Tocotrienol Alleviates Inflammation And Oxidative Stress In A Rat Model Of Spinal Cord Injury Via Suppression Of Transforming Growth Factor-β.

    DOI: 10.3892/etm.2017.4505

    PMID: 28672950

    Sample: Serum
  15. Molecular Medicine Reports (2015) IF: 1.692
    Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism.

    DOI: 10.3892/mmr.2015.4425

    PMID: 26458544

    Sample: Serum
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