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Rat NGF(Nerve Growth Factor) ELISA Kit

Citations(6)

Cat.No.:E-EL-R0652
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	39.06-2500 pg/mL
Sensitivity	23.44 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat NGF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NGF. You can calculate the concentration of Rat NGF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NGF were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	133.12	309.15	1103.70	127.82	305.03	1172.82
Standard deviation	8.52	17.78	53.86	6.62	12.57	56.76
CV (%)	6.40	5.75	4.88	5.18	4.12	4.84

Recovery

The recovery of Rat NGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	99
EDTA plasma (n=8)	93-108	99
Cell culture media (n=8)	90-107	98

Linearity

Samples were spiked with high concentrations of Rat NGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Beta-NGF, HSAN5, NGFB, NGF-B
Research Area	Cancer, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BEHAVIOURAL BRAIN RESEARCH (2019) IF: 2.77

Voluntary, involuntary and forced exercises almost equally reverse behavioral impairment by regulating hippocampal neurotrophic factors and oxidative stress in experimental Alzheimer’s disease model

DOI: 10.1016/j.bbr.2019.02.030
	Sample: Tissue Homogenate

NEUROSCIENCE LETTERS (2022) IF: 3.046

Differential effects of voluntary and forced exercise trainings on spatial learning ability and hippocampal biomarkers in aged female rats

DOI: 10.1016/j.neulet.2022.136499
PMID: 35121056	Sample: Hippocampus

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019) IF: 3.091

Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

DOI: 10.1111/aji.13096
	Sample: Serum

LIFE SCIENCES (2019) IF: 3.448

Effects of noopept on cognitive functions and pubertal process in rats with diabetes

DOI: 10.1016/j.lfs.2019.116698
PMID: 31356906	Sample: Serum,Tissue Homogenate

International Journal of Biological Sciences (2017) IF: 3.873

Dual Delivery of NGF and bFGF Coacervater Ameliorates Diabetic Peripheral Neuropathy via Inhibiting Schwann Cells Apoptosis

DOI: 10.7150/ijbs.18636
	Sample: Cell Culture Supernatant

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 5.19

Therapeutic antischizophrenic activity of prodigiosin and selenium co-supplementation against amphetamine hydrochloride-induced behavioural changes and oxidative, inflammatory, and apoptotic challenges in rats

DOI: 10.1007/s11356-022-22409-x
PMID: 36048389	Sample: Brain Tissue

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