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Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit

  • Cat.No.:E-EL-R0520

  • Reactivity: Rat

To Purchase E-EL-R0520

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NOS2/iNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NOS2/iNOS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.00 2.70 8.10 1.10 2.70 7.70
Standard deviation 0.10 0.10 0.40 0.10 0.10 0.40
CV (%) 10.00 3.70 4.94 9.09 3.70 5.19


The recovery of Rat NOS2/iNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 89-101 96
Cell culture media (n=8) 89-102 94


Samples were spiked with high concentrations of Rat NOS2/iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCancer, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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  1. Reproductive Toxicology (2020) IF: 3.121
    Obesity-induced testicular oxidative stress, inflammation and apoptosis: Protective and therapeutic effects of orlistat

    DOI: 10.1016/j.reprotox.2020.05.009

    PMID: 32450208

    Sample: Tissue homogenate
  2. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Trichosanthes kirilowii lectin ameliorates streptozocin-induced kidney injury via modulation of the balance between M1/M2 phenotype macrophage

    DOI: 10.1016/j.biopha.2018.10.060

    PMID: 30396096

    Sample: Serum
  3. Journal of cellular physiology (2019) IF: 3.923
    Physiological and structural changes of the lung tissue in male albino rat exposed to immobilization stress

    DOI: 10.1002/jcp.27594

    PMID: 30317604

    Sample: Tissue homogenate
  4. Molecular vision (2018) IF: 2.219
    Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress

    PMID: 30090013

    Sample: Tissue homogenate
  5. Plos One (2017) IF: 2.806
    Reduction Of Oxidative-Nitrosative Stress Underlies Anticataract Effect Of Topically Applied Tocotrienol In Streptozotocin-Induced Diabetic Rats.

    DOI: 10.1371/journal.pone.0174542

    PMID: 28350848

    Sample: Tissue homogenate
  6. Molecules (2016) IF: 2.861
    Zerumbone, A Bioactive Sesquiterpene, Ameliorates Diabetes-Induced Retinal Microvascular Damage Through Inhibition Of Phospho-P38 Mitogen-Activated Protein Kinase And Nuclear Factor-κB Pathways.

    DOI: 10.3390/molecules21121708

    PMID: 27973425

    Sample: Tissue homogenate
  7. Cytotechnology (2019) IF: 1.461
    Parkia Speciosa Empty Pod Extract Exerts Anti-Inflammatory Properties by Modulating NFκB and MAPK Pathways in Cardiomyocytes Exposed to Tumor Necrosis Factor-α

    DOI: 10.1007/s10616-018-0267-8

    PMID: 30600464

    Sample: Cell culture supernatant
  8. Journal of plastic surgery and hand surgery (2019) IF: 1.1
    Transferring the protective effect of remote ischemic preconditioning on skin flap among rats by blood Serum

    DOI: 10.1080/2000656X.2019.1582422

    PMID: 30888241

    Sample: Serum
  9. Molecular Medicine Reports (2015) IF: 1.692
    Candesartan Ameliorates Acute Myocardial Infarction In Rats Through Inducible Nitric Oxide Synthase, Nuclear FactorκB, Monocyte Chemoattractant Protein1, Activator Protein1 And Restoration Of Heat Shock Protein 72.

    DOI: 10.3892/mmr.2015.4432

    PMID: 26499133

    Sample: Serum
  10. Clinics in Shoulder and Elbow (2019)
    The Evaluation of Exogenous Melatonin Administration in Supraspinatus Overuse Tendinopathy in an Experimental Rat Model

    DOI: 10.5397/cise.2019.22.2.79

    Sample: Plasma
  11. GSC Biological and Pharmaceutical Sciences (2019)
    Protective effects of naringin in indomethacin-induced gastric ulcer in rats

    DOI: 10.30574/gscbps.2019.8.2.0132

    Sample: Tissue homogenate
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