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Rat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit

Citations(25)

  • Cat.No.:E-EL-R0520

  • Reactivity: Rat
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Manual MSDS

To Purchase E-EL-R0520

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NOS2/iNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NOS2/iNOS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.00 2.70 8.10 1.10 2.70 7.70
Standard deviation 0.10 0.10 0.40 0.10 0.10 0.40
CV (%) 10.00 3.70 4.94 9.09 3.70 5.19

Recovery

The recovery of Rat NOS2/iNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma (n=8) 89-101 96
Cell culture media (n=8) 89-102 94

Linearity

Samples were spiked with high concentrations of Rat NOS2/iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsNOS2A, HEP-NOS, I-NOS
Research AreaCancer, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Antioxidants (2022) IF: 6.313
    Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model

    DOI: 10.3390/antiox11020255

    PMID: 35204140

    		 Sample: Testis
  2. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 6.53
    Trichosanthes kirilowii lectin ameliorates streptozocin-induced kidney injury via modulation of the balance between M1/M2 phenotype macrophage

    DOI: 10.1016/j.biopha.2018.10.060

    PMID: 30396096

    		 Sample: Serum
  3. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953
    Metabolites of gut microbiota fermenting Poria cocos polysaccharide alleviates chronic nonbacterial prostatitis in rats

    DOI: 10.1016/j.ijbiomac.2022.04.029

    PMID: 35398386

    		 Sample: prostate
  4. Frontiers in Neuroscience (2022) IF: 5.152
    Inhibition of BET Protein Function Suppressed the Overactivation of the Canonical NF-κB Signaling Pathway in 6-OHDA-Lesioned Rat Model of Levodopa-Induced Dyskinesia

    DOI: 10.3389/fnins.2022.896322

    PMID: 35801173

    		 Sample: striatal tissue
  5. Chinese Medicine (2022) IF: 5.455
    Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway

    DOI: 10.1186/s13020-021-00562-8

    PMID: 35123524

    		 Sample: serum
  6. Frontiers in Pharmacology (2021) IF: 5.811
    Parkia speciosa Hassk. Empty Pod Extract Alleviates Angiotensin II-Induced Cardiomyocyte Hypertrophy in H9c2 Cells by Modulating the Ang II/ROS/NO Axis and MAPK Pathway

    DOI: 10.3389/fphar.2021.741623
    Sample: H9c2 cell
  7. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Rutin Modulates MAPK Pathway Differently from Quercetin in Angiotensin II-Induced H9c2 Cardiomyocyte Hypertrophy

    DOI: 10.3390/ijms22105063

    PMID: 34064664

    		 Sample: Cell lysate
  8. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021) IF: 4.31
    Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet

    DOI: 10.1152/ajpendo.00093.2021

    PMID: 34229480

    		 Sample: serum,testis
  9. Scientific Reports (2022) IF: 4.38
    Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats

    DOI: 10.1038/s41598-022-09380-w

    PMID: 35354886

    		 Sample: plasma
  10. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats

    DOI: 10.1016/j.biopha.2020.110781

    PMID: 33152939

    		 Sample: Tissue homogenate
  11. Biomedicines (2022) IF: 4.757
    The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model

    DOI: 10.3390/biomedicines10102508

    PMID: 36289770

    		 Sample: liver tissue
  12. REPRODUCTIVE TOXICOLOGY (2020) IF: 3.121
    Obesity-induced testicular oxidative stress, inflammation and apoptosis: Protective and therapeutic effects of orlistat

    DOI: 10.1016/j.reprotox.2020.05.009

    PMID: 32450208

    		 Sample: Tissue homogenate
  13. REPRODUCTION (2020) IF: 3.206
    Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

    DOI: 10.1530/REP-20-0381

    PMID: 33112813

    		 Sample: testis
  14. METABOLIC BRAIN DISEASE (2021) IF: 3.584
    Protective mechanism of Syringic acid in an experimental model of Parkinson’s disease

    DOI: 10.1007/s11011-021-00704-9
    Sample: Tissue homogenate
  15. CELL STRESS & CHAPERONES (2021) IF: 3.667
    Protective effect of hydrogen sulfide against stress-induced lung injury: involvement of Nrf2, NFκB/iNOS, and HIF-1α signaling pathways

    DOI: 10.1007/s12192-021-01248-8

    PMID: 34881408

    		 Sample: lung
  16. JOURNAL OF CELLULAR PHYSIOLOGY (2018) IF: 3.923
    Physiological and structural changes of the lung tissue in male albino rat exposed to immobilization stress

    DOI: 10.1002/jcp.27594

    PMID: 30317604

    		 Sample: Tissue homogenate
  17. MOLECULAR VISION (2018) IF: 2.219
    Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress

    PMID: 30090013

    		 Sample: retinal tissues
  18. MOLECULES (2016) IF: 2.465
    Zerumbone, a Bioactive Sesquiterpene, Ameliorates Diabetes-Induced Retinal Microvascular Damage through Inhibition of Phospho-p38 Mitogen-Activated Protein Kinase and Nuclear Factor-κB Pathways

    DOI: 10.3390/molecules21121708
    Sample: Tissue homogenate
  19. PLoS One (2017) IF: 2.806
    Reduction of oxidative-nitrosative stress underlies anticataract effect of topically applied tocotrienol in streptozotocin-induced diabetic rats

    DOI: 10.1371/journal.pone.0174542
    Sample: Tissue homogenate
  20. HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
    Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat:

    DOI: 10.1177/09603271211026729
    Sample: liver
  21. NEUROSCIENCE RESEARCH (2023) IF: 2.904
    Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: insights into the role of adenosine A1 receptors

    DOI: 10.1016/j.neures.2023.02.004
    Sample: retinal
  22. Journal of Plastic Surgery and Hand Surgery (2019) IF: 1.037
    Transferring the protective effect of remote ischemic preconditioning on skin flap among rats by blood serum

    DOI: 10.1080/2000656X.2019.1582422

    PMID: 30888241

    		 Sample: Serum
  23. Anatolian Journal of Cardiology (2016) IF: 1.141
    Cardioprotective effects of Viscum album L. ssp. album (Loranthaceae) on isoproterenol-induced heart failure via regulation of the nitric oxide pathway in rats

    DOI: 10.14744/AnatolJCardiol.2016.6780
    Sample: plasma
  24. Molecular Medicine Reports (2015) IF: 1.554
    Candesartan ameliorates acute myocardial infarction in rats through inducible nitric oxide synthase, nuclear factor‑κB, monocyte chemoattractant protein‑1, activator protein‑1 and restoration of heat shock protein 72

    DOI: 10.3892/mmr.2015.4432
    Sample: Serum
  25. CYTOTECHNOLOGY (2019) IF: 1.672
    empty pod extract exerts anti-inflammatory properties by modulating NFκB and MAPK pathways in cardiomyocytes exposed to tumor necrosis factor-α

    DOI: 10.1007/s10616-018-0267-8
    Sample: Cell culture supernatant
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