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Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA Kit

  • Cat.No.:E-EL-R0367

  • Reactivity: Rat

To Purchase E-EL-R0367

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NOS3/eNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS3/eNOS. You can calculate the concentration of Rat NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NOS3/eNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NOS3/eNOS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 48.40 111.20 492.00 53.00 121.10 496.00
Standard deviation 3.00 6.70 25.10 2.70 5.20 17.90
CV (%) 6.20 6.03 5.10 5.09 4.29 3.61


The recovery of Rat NOS3/eNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma (n=8) 86-102 93
Cell culture media (n=8) 95-106 101


Samples were spiked with high concentrations of Rat NOS3/eNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q62600
Research AreaCancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


    Quince extract resists atherosclerosis in rats by down-regulating the EGFR/PI3K/Akt/GSK-3β pathway

    DOI: 10.1016/j.biopha.2023.114330

    Sample: serum
    Renoprotective and therapeutic effects of newly water, ethanol, and butanol ginseng fractions in hypertensive and chronic kidney disease with L-NAME

    DOI: 10.1016/j.biopha.2021.111978

    PMID: 34411920

    Sample: serum
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    Sample: heart
  4. LIFE SCIENCES (2022) IF: 5.037
    Suppression of PCSK9/NF-kB-dependent pathways by acetate ameliorates cardiac inflammation in a rat model of polycystic ovarian syndrome

    DOI: 10.1016/j.lfs.2022.120560

    PMID: 35452635

    Sample: plasma
  5. Nutrition & Metabolism (2021) IF: 4.169
    Diet-induced prediabetes: effects of exercise treatment on risk factors for cardiovascular complications

    DOI: 10.1186/s12986-021-00573-0

    PMID: 33888141

    Sample: Plasma
  6. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690

    Sample: liver
    Nitric oxide modulates tapentadol antinociceptive tolerance and physical dependence

    DOI: 10.1016/j.ejphar.2021.174245

    PMID: 34126091

    Sample: lumbar
  8. Life-Basel (2022) IF: 3.251
    Evaluation of the Cardiovascular Effects of Coriandrum sativum and Citrus limon to Treat Arsenic-Induced Endothelial Damage and Hypertension in Rats

    DOI: 10.3390/life12111842

    Sample: serum
    var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system

    DOI: 10.1007/s11033-019-04730-w

    Sample: serum,urine
  10. MOLECULAR VISION (2018) IF: 2.219
    Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress

    PMID: 30090013

    Sample: retinal tissues
  11. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    Sample: Plasma,Tissue homogenate
  12. Journal of Plastic Surgery and Hand Surgery (2019) IF: 1.037
    Transferring the protective effect of remote ischemic preconditioning on skin flap among rats by blood serum

    DOI: 10.1080/2000656X.2019.1582422

    PMID: 30888241

    Sample: Serum
  13. ARYA Atherosclerosis (2021)
    The effect of subchronic cigarette smoke exposure on oxidative stress parameters and endothelial nitric oxide synthase in a rat aorta

    DOI: 10.22122/arya.v17i0.2150

    Sample: aortas
  14. Chinese Herbal Medicines (2019)
    Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats

    DOI: 10.1016/j.chmed.2018.12.004

    Sample: Plasma,Serum,Tissue homogenate
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