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Rat NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Cat.No.:E-EL-R3023
Reactivity: Rat

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48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat NT-proBNP in samples. No significant cross-reactivity or interference between Rat NT-proBNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat NT-proBNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NT-proBNP. You can calculate the concentration of Rat NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NT-proBNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NT-proBNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	184.27	457.87	1859.66	182.33	410.06	1884.75
Standard deviation	9.58	21.15	91.50	9.32	19.19	88.58
CV (%)	5.20	4.62	4.92	5.11	4.68	4.70

Recovery

The recovery of Rat NT-proBNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-105	99
EDTA plasma (n=8)	97-105	102
Cell culture media (n=8)	97-109	101

Linearity

Samples were spiked with high concentrations of Rat NT-proBNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	NT-Pro-BNP
Research Area	Cancer, Cardiovascular, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Cardiovascular Medicine (2021) IF: 6.05

Early Passive Leg Movement Prevents Against the Development of Heart Failure With Preserved Ejection Fraction in Rats

DOI: 10.3389/fcvm.2021.655009
PMID: 33969019	Sample: Serum

BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53

Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway

DOI: 10.1016/j.biopha.2020.111184
PMID: 33418305	Sample: Plasma

TOXICOLOGY AND APPLIED PHARMACOLOGY (2021) IF: 4.219

Phenomapping for classification of doxorubicin-induced cardiomyopathy in rats

DOI: 10.1016/j.taap.2021.115579
PMID: 34015281	Sample: Plasma

Frontiers in Pharmacology (2020) IF: 4.225

Tsantan Sumtang Restored Right Ventricular Function in Chronic Hypoxia-Induced Pulmonary Hypertension Rats

DOI: 10.3389/fphar.2020.607384
PMID: 33536917	Sample: Plasma

SAUDI PHARMACEUTICAL JOURNAL (2021) IF: 4.33

Cinnamomum zeylanicum Blume (Ceylon cinnamon) bark extract attenuates doxorubicin induced cardiotoxicity in Wistar rats

DOI: 10.1016/j.jsps.2021.06.004
PMID: 34408544	Sample: serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556

Age- and Phenotype-Dependent Changes in Circulating MMP-2 and MMP-9 Activities in Normotensive and Hypertensive Rats

DOI: 10.3390/ijms21197286
PMID: 33023122	Sample: plasma

VOX SANGUINIS (2021) IF: 2.144

Differential effects of speed and volume on transfusion-associated circulatory overload: A randomized study in rats

DOI: 10.1111/vox.13191
PMID: 34396543	Sample: serum

Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63

Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression

DOI: 10.1155/2021/6635876
PMID: 33603819	Sample: Serum

Journal of Healthcare Engineering (2022) IF: 2.682

The Difference between Sacubitril Valsartan and Valsartan on Vascular Endothelial Function, APN, MMP-9, and BNP Levels in Patients with Hypertension and Chronic Heart Failure

DOI: 10.1155/2022/9494981
PMID: 35222898	Sample: blood

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2019) IF: 2.813

Synergistic effect of Aconiti Lateralis Radix Praeparata water-soluble alkaloids and Ginseng Radix et Rhizoma total ginsenosides compatibility on acute heart failure rats

DOI: 10.1016/j.jchromb.2019.121935
PMID: 31877430	Sample: Serum

PHARMACEUTICAL BIOLOGY (2020) IF: 2.971

Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism

DOI: 10.1080/13880209.2020.1778043
	Sample: serum

Anatolian Journal of Cardiology (2016) IF: 1.141

Cardioprotective effects of Viscum album L. ssp. album (Loranthaceae) on isoproterenol-induced heart failure via regulation of the nitric oxide pathway in rats

DOI: 10.14744/AnatolJCardiol.2016.6780
	Sample: plasma

Evidence-based Complementary and Alternative Medicine (2020) IF: 1.813

Cardioprotective Potential of Murraya koenigii (L.) Spreng. Leaf Extract against Doxorubicin-Induced Cardiotoxicity in Rats

DOI: 10.1155/2020/6023737
PMID: 32308710	Sample: Serum

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