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Current Position:Home>>Kits>>ELISA Kits>> Rat PRL(Prolactin) ELISA Kit

Rat PRL(Prolactin) ELISA Kit

Citations(17)

  • Cat.No.:E-EL-R3006

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.13-200 ng/mL
Sensitivity 1.88 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat PRL in samples. No significant cross-reactivity or interference between Rat PRL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat PRL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PRL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PRL. You can calculate the concentration of Rat PRL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PRL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PRL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 8.90 20.68 83.80 8.89 23.84 81.45
Standard deviation 0.45 1.02 3.61 0.47 1.17 4.23
CV (%) 5.06 4.93 4.31 5.29 4.91 5.19

Recovery

The recovery of Rat PRL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-112 105
EDTA plasma (n=8) 88-99 92
Cell culture media (n=8) 86-97 94

Linearity

Samples were spiked with high concentrations of Rat PRL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsLactotrope, LTH, Luteotropic Hormone
Research AreaCancer, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Computational and Structural Biotechnology Journal (2020) IF: 6.018
    Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

    DOI: 10.1016/j.csbj.2020.11.009

    PMID: 33304464

    		 Sample: serum
  2. International Journal of Nanomedicine (2021) IF: 6.4
    Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model

    DOI: 10.2147/IJN.S302161

    PMID: 34531655

    		 Sample: serum
  3. JOURNAL OF MOLECULAR ENDOCRINOLOGY (2021) IF: 5.098
    Pathological changes and oxidative stress of the HPG axis in hypothyroid rat

    DOI: 10.1530/JME-21-0095

    PMID: 34270447

    		 Sample: serum
  4. Frontiers in Endocrinology (2022) IF: 5.555
    PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas

    DOI: 10.3389/fendo.2021.706909
    Sample: serum,MMQ cell
  5. ENDOCRINE-RELATED CANCER (2021) IF: 5.678
    Microglial NLRP3 inflammasome activation-mediated inflammation promotes prolactinoma development

    DOI: 10.1530/ERC-21-0137

    PMID: 33974557

    		 Sample: serum
  6. TOXICOLOGY (2020) IF: 4.099
    Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats

    DOI: 10.1016/j.tox.2020.152556

    PMID: 32758512

    		 Sample: Serum,Tissue homogenate
  7. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Total barley maiya alkaloids inhibit prolactin secretion by acting on dopamine D2 receptor and protein kinase A targets

    DOI: 10.1016/j.jep.2021.113994

    PMID: 33711439

    		 Sample: Cell culture supernatant
  8. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
    Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats

    DOI: 10.1007/s11356-020-10324-y

    PMID: 32812151

    		 Sample: Serum,Tissue homogenate
  9. BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
    Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring

    DOI: 10.1016/j.bbr.2022.114082

    PMID: 36041571

    		 Sample: serum
  10. HORMONES AND BEHAVIOR (2022) IF: 3.587
    The elusive role of prolactin in the sociality of the naked mole-rat

    DOI: 10.1016/j.yhbeh.2022.105196

    PMID: 35597054

    		 Sample: Urinary
  11. ENDOCRINE (2022) IF: 3.633
    Beneficial effects of irisin in experimental paroxetine-induced hyperprolactinemia

    DOI: 10.1007/s12020-022-03010-1

    PMID: 35226247

    		 Sample: serum
  12. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021) IF: 3.652
    Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats

    DOI: 10.1002/jbt.22741
    Sample: Serum
  13. Evidence-based Complementary and Alternative Medicine (2018) IF: 2.064
    Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment

    DOI: 10.1155/2018/3858209
    Sample: serum
  14. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    Cannabinoid-deficient Benin republic hemp ( Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

    DOI: 10.1186/s12906-019-2541-5
    Sample: plasma
  15. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    Chlorogenic acid co-administration abates tamoxifen-mediated reproductive toxicities in male rats: An experimental approach

    DOI: 10.1111/jfbc.13615

    PMID: 33491243

    		 Sample: Serum
  16. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2020) IF: 1.665
    Inhibition of lncRNA-UCA1 suppresses pituitary cancer cell growth and prolactin (PRL) secretion via attenuating glycolysis pathway

    DOI: 10.1007/s11626-020-00494-x

    PMID: 32909148

    		 Sample: Cell culture supernatant
  17. World Neurosurgery (2019) IF: 1.723
    AFAP1-AS1 Promotes Proliferation of Pituitary Adenoma Cells through miR-103a-3p to Activate PI3K/AKT Signaling Pathway

    DOI: 10.1016/j.wneu.2019.07.032
    Sample: GH3 cells,MMQ cells
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