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Rat S100B(S100 Calcium Binding Protein B) ELISA Kit

Cat.No.:E-EL-R0868
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat S100B in samples. No significant cross-reactivity or interference between Rat S100B and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat S100B concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat S100B. You can calculate the concentration of Rat S100B in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat S100B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat S100B were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	201.30	463.70	1488.10	188.10	449.20	1497.50
Standard deviation	12.50	25.00	59.50	12.80	23.40	80.90
CV (%)	6.21	5.39	4.00	6.80	5.21	5.40

Recovery

The recovery of Rat S100B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-104	96
EDTA plasma (n=8)	87-99	92
Cell culture media (n=8)	95-106	100

Linearity

Samples were spiked with high concentrations of Rat S100B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	S100-B, NEF, S100Beta
Research Area	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of the American Heart Association (2022) IF: 6.106
Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia‐Induced Cardiac Arrest and Resuscitation Rat Model

DOI: 10.1161/JAHA.122.027685

Sample: blood
Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
Neuroprotective Effect of the Inhibitor Salubrinal after Cardiac Arrest in a Rodent Model

DOI: 10.1155/2020/7468738

PMID: 32064028
Sample: Serum
Journal of the American Heart Association (2020) IF: 4.605
Cerebral Blood Flow–Guided Manipulation of Arterial Blood Pressure Attenuates Hippocampal Apoptosis After Asphyxia‐Induced Cardiac Arrest in Rats

DOI: 10.1161/JAHA.120.016513

Sample: serum
PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221

PMID: 35635784
Sample: serum
NEUROCHEMICAL RESEARCH (2019) IF: 3.996
Triptolide Improves Cognitive Dysfunction in Rats with Vascular Dementia by Activating the SIRT1/PGC-1α Signaling Pathway

DOI: 10.1007/s11064-019-02831-3

Sample: serum
INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020) IF: 2.107
Aging protects rat cortical slices against to oxygen-glucose deprivation induced damage

DOI: 10.1080/00207454.2020.1730830

Sample: cortical
European Journal of Trauma and Emergency Surgery (2020) IF: 2.139
Expression of Notch and Wnt/β-catenin signaling pathway in acute phase severe brain injury rats and the effect of exogenous thyroxine on those pathways

DOI: 10.1007/s00068-020-01359-4

Sample: blood
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 2.559
Mitochondrial dynamics and protective effects of a mitochondrial division inhibitor, Mdivi-1, in lipopolysaccharide-induced brain damage

DOI: 10.1016/j.bbrc.2018.01.136

Sample: plasma
BEHAVIOURAL BRAIN RESEARCH (2019) IF: 2.77
Long-term cognitive impairment without diffuse axonal injury following repetitive mild traumatic brain injury in rats

DOI: 10.1016/j.bbr.2019.112268

Sample: serum
Molecular Medicine Reports (2020) IF: 2.952
Effect of ultra‑wide‑band electromagnetic pulses on blood‑brain barrier permeability in rats

DOI: 10.3892/mmr.2020.11382

Sample: Serum
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2019) IF: 1.351
Electroacupuncture reduces astrocyte number and oxidative stress in aged rats with surgery-induced cognitive dysfunction:

DOI: 10.1177/0300060519860026

Sample: Serum
MEDICAL SCIENCE MONITOR (2019) IF: 1.98
Expression of S100B Protein in Ischemia/Reperfusion-Induced Brain Injury After Cyclosporine Therapy: A Biochemical Serum Marker with Prognostic Value?

DOI: 10.12659/MSM.912810

Sample: serum

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