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Rat S100B(S100 Calcium Binding Protein B) ELISA Kit

  • Cat.No.:E-EL-R0868

  • Reactivity: Rat

To Purchase E-EL-R0868

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat S100B in samples. No significant cross-reactivity or interference between Rat S100B and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat S100B concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat S100B. You can calculate the concentration of Rat S100B in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat S100B were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat S100B were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 201.30 463.70 1488.10 188.10 449.20 1497.50
Standard deviation 12.50 25.00 59.50 12.80 23.40 80.90
CV (%) 6.21 5.39 4.00 6.80 5.21 5.40


The recovery of Rat S100B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 96
EDTA plasma (n=8) 87-99 92
Cell culture media (n=8) 95-106 100


Samples were spiked with high concentrations of Rat S100B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsS100-B, NEF, S100Beta
Research AreaCancer, Cell Biology, Epigenetics and Nuclear Signaling, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of the American Heart Association (2022) IF: 6.106
    Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia‐Induced Cardiac Arrest and Resuscitation Rat Model

    DOI: 10.1161/JAHA.122.027685

    Sample: blood
  2. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Neuroprotective Effect of the Inhibitor Salubrinal after Cardiac Arrest in a Rodent Model

    DOI: 10.1155/2020/7468738

    PMID: 32064028

    Sample: Serum
  3. Journal of the American Heart Association (2020) IF: 4.605
    Cerebral Blood Flow–Guided Manipulation of Arterial Blood Pressure Attenuates Hippocampal Apoptosis After Asphyxia‐Induced Cardiac Arrest in Rats

    DOI: 10.1161/JAHA.120.016513

    Sample: serum
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    Sample: serum
    Triptolide Improves Cognitive Dysfunction in Rats with Vascular Dementia by Activating the SIRT1/PGC-1α Signaling Pathway

    DOI: 10.1007/s11064-019-02831-3

    Sample: serum
    Aging protects rat cortical slices against to oxygen-glucose deprivation induced damage

    DOI: 10.1080/00207454.2020.1730830

    Sample: cortical
  7. European Journal of Trauma and Emergency Surgery (2020) IF: 2.139
    Expression of Notch and Wnt/β-catenin signaling pathway in acute phase severe brain injury rats and the effect of exogenous thyroxine on those pathways

    DOI: 10.1007/s00068-020-01359-4

    Sample: blood
    Mitochondrial dynamics and protective effects of a mitochondrial division inhibitor, Mdivi-1, in lipopolysaccharide-induced brain damage

    DOI: 10.1016/j.bbrc.2018.01.136

    Sample: plasma
    Long-term cognitive impairment without diffuse axonal injury following repetitive mild traumatic brain injury in rats

    DOI: 10.1016/j.bbr.2019.112268

    Sample: serum
  10. Molecular Medicine Reports (2020) IF: 2.952
    Effect of ultra‑wide‑band electromagnetic pulses on blood‑brain barrier permeability in rats

    DOI: 10.3892/mmr.2020.11382

    Sample: Serum
    Electroacupuncture reduces astrocyte number and oxidative stress in aged rats with surgery-induced cognitive dysfunction:

    DOI: 10.1177/0300060519860026

    Sample: Serum
    Expression of S100B Protein in Ischemia/Reperfusion-Induced Brain Injury After Cyclosporine Therapy: A Biochemical Serum Marker with Prognostic Value?

    DOI: 10.12659/MSM.912810

    Sample: serum
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