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Rat SDF-1/CXCL12(Stromal Cell Derived Factor 1) ELISA Kit

Cat.No.:E-EL-R3027
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Rat SDF-1/CXCL12 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1/CXCL12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1/CXCL12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDF-1/CXCL12. You can calculate the concentration of Rat SDF-1/CXCL12 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat SDF-1/CXCL12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat SDF-1/CXCL12 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.89	2.29	8.82	0.93	2.07	8.78
Standard deviation	0.05	0.11	0.39	0.05	0.10	0.47
CV (%)	5.62	4.80	4.42	5.38	4.83	5.35

Recovery

The recovery of Rat SDF-1/CXCL12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-106	103
EDTA plasma (n=8)	86-101	93
Cell culture media (n=8)	83-96	90

Linearity

Samples were spiked with high concentrations of Rat SDF-1/CXCL12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	SDF1, CXCL12, IRH, PBSF, SCYB12, TLSF, TPAR1
Research Area	Cancer, Cardiovascular, Immunology, Microbiology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Stem Cell Research & Therapy (2022) IF: 6.832
Sequential transplantation of exosomes and mesenchymal stem cells pretreated with a combination of hypoxia and Tongxinluo efficiently facilitates cardiac repair

DOI: 10.1186/s13287-022-02736-z

PMID: 35130979
Sample: myocardium
Pharmaceuticals (2022) IF: 5.215
Repositioning Linagliptin for the Mitigation of Cadmium-Induced Testicular Dysfunction in Rats: Targeting HMGB1/TLR4/NLRP3 Axis and Autophagy

DOI: 10.3390/ph15070852

PMID: 35890148
Sample: testicular tissue
Stem Cells Translational Medicine (2019) IF: 5.962
Optimization of Timing and Times for Administration of Atorvastatin‐Pretreated Mesenchymal Stem Cells in a Preclinical Model of Acute Myocardial Infarction

DOI: 10.1002/sctm.19-0013

Sample: Tissue homogenate
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2015) IF: 4.101
Effect of SDF-1/CXCR4 axis on the migration of transplanted bone mesenchymal stem cells mobilized by erythropoietin toward lesion sites following spinal cord injury

DOI: 10.3892/ijmm.2015.2344

Sample: Tissue homogenate
JOURNAL OF CELL SCIENCE (2018) IF: 4.401
The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs

DOI: 10.1242/jcs.216135

Sample: Tissue homogenate
EXPERIMENTAL NEUROLOGY (2018) IF: 4.483
Oncostatin M-induced upregulation of SDF-1 improves Bone marrow stromal cell migration in a rat middle cerebral artery occlusion stroke model

DOI: 10.1016/j.expneurol.2018.09.005

PMID: 30213507
Sample: Cell culture supernatant
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
Low‐energy shock wave pretreatment recruit circulating endothelial progenitor cells to attenuate renal ischaemia reperfusion injury

DOI: 10.1111/jcmm.15678

Sample: Serum,Tissue homogenate
Stem Cell Research & Therapy (2019) IF: 4.627
Combinatorial treatment of acute myocardial infarction using stem cells and their derived exosomes resulted in improved heart performance

DOI: 10.1186/s13287-019-1353-3

PMID: 31601262
Sample: Cell culture supernatant,Tissue homogenate
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2019) IF: 3.559
Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture

DOI: 10.1016/j.abb.2019.108108

PMID: 31550444
Sample: Cell lysate
REPRODUCTION (2021) IF: 3.906
CXCL12 and its receptors regulate granulosa cell apoptosis in PCOS rats and human KGN tumor cells

DOI: 10.1530/REP-20-0451

PMID: 33258800
Sample: Serum,Cell culture supernatant(ovarian granulosa c

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