Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    <
    >
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-R1424

      Synonyms:ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Rat

      Detection Range: 0.16~10 ng/mL

      Sensitivity: 0.10 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Rat SOD1 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat SOD1. During the reaction, Rat SOD1 in the sample or standard competes with a fixed amount of Rat SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SOD1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive
      Format 96T
      Assay time 2.0h
      Reactivity Rat
      Detection Method Colormetric
      Detection Range 0.16—10 ng/mL
      Sensitivity 0.10 ng/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Rat SOD1 in samples. No significant cross-reactivity or interference between Rat SOD1 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL) O.D Average
      10 0.382
      0.44
      0.411
      5 0.512
      0.54
      0.526
      2.5 0.734
      0.72
      0.727
      1.25 1.032
      1.05
      1.041
      0.63 1.453
      1.431
      1.442
      0.31 1.882
      1.854
      1.868
      0.16 2.181
      2.197
      2.189
      0 2.685
      2.691
      2.688

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat SOD1 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat SOD1 were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (ng/mL) 0.50 1.20 3.40 0.50 1.30 3.60
      Standard deviation 0.03 0.07 0.12 0.03 0.07 0.13
      C V (%) 6.00 5.83 3.53 6.00 5.38 3.61

      Recovery

      The recovery of Rat SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 91-102 97
      EDTA plasma (n=5) 91-104 97
      Cell culture media (n=5) 89-105 96

      Linearity

      Samples were spiked with high concentrations of Rat SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 90-104 90-104 92-105
      Average (%) 98 96 100
      1:4 Range (%) 84-97 91-103 100-115
      Average (%) 90 98 107
      1:8 Range (%) 87-99 94-104 95-107
      Average (%) 92 99 101
      1:16 Range (%) 86-98 89-102 96-110
      Average (%) 93 96 103

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      1.Add 50 μL standard or sample to each well.

      2.Immediately add 50 μL Biotinylated Detection Ab to each well.

      3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.

      6.Add 50 μL Stop Solution.

      7.Read at 450 nm immediately. Calculation of results.

      Citations

      1. Publication: Luo Y, Fu C, Wang Z, et al. Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And Anti‑Inflammatory Effects, And Inhibition Of The p38‑MAPK Mechanism[J]. Molecular Medicine Reports, 2015, 12: 8294-8300.
        Sample Type: Serum
      2. Publication: Xun C, Mamat M, Guo H, et al. Tocotrienol Alleviates Inflammation And Oxidative Stress In A Rat Model Of Spinal Cord Injury Via Suppression Of Transforming Growth Factor-β[J]. Experimental and Therapeutic Medicine, 2017, 14(1): 431-438.
        Sample Type: Serum
      3. Publication: Bahadır A, Ceyhan A, Gergin Ö Ö, et al. Protective Effects of Curcumin and Beta-carotene on Cisplatin-induced Cardiotoxicity: An Experimental Rat Model[J]. Anatolian Journal of Cardiology, 2018, 19(3): 213.
      4. Publication: Quadri J A, Sarwar S, Kar P, et al. Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings[J]. Toxicology, 2018, 406: 44-57.
        Sample Type: Rat
      5. Publication: Yuan X, Fei F, Sun H, et al. Tanshinol borneol ester on nanostructured lipid carriers has longer brain and systemic effector retention and better antioxidant activity in vivo[J]. International Journal of Nanomedicine, 2018, 13: 2265.
        Sample Type: Rat
      6. Publication: Peng Zeng, Yan Shi, Xiao-Ming Wang, Li Lin, Yan-Jun Du, Na Tang, Qun Wang, Ying-Yan Fang, Jian-Zhi Wang, Xin-Wen Zhou, Youming Lu, Qing Tian; Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats, International Journal of Neuropsychopharmacology, pyy090.
        Sample Type: Rat
      Show AllShorten
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought