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Cat.No.:E-EL-R1424
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 0.16-10 ng/mL |
Sensitivity | 0.10 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.50 | 1.20 | 3.40 | 0.50 | 1.30 | 3.60 |
Standard deviation | 0.03 | 0.07 | 0.12 | 0.03 | 0.07 | 0.13 |
CV (%) | 6.00 | 5.83 | 3.53 | 6.00 | 5.38 | 3.61 |
The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-102 | 97 |
EDTA plasma(n=8) | 91-104 | 97 |
Cell culture media(n=8) | 89-105 | 96 |
Samples were spiked with high concentrations of Rat SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt: P07632 |
Synonyms | ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn] |
Research Area | Cancer, Cell Biology, Metabolism, Signal transduction |
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1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
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Sample: brain |
PMID: 35671584 |
Sample: serum |
DOI: 10.1093/ijnp/pyy090 |
|
Sample: Plasma,Tissue homogenate |
PMID: 31425004 |
Sample: Serum,Cell culture medium |
Sample: serum |
PMID: 35029831 |
Sample: hepatic tissue |
DOI: 10.1111/jcmm.15351 |
|
Sample: Primary cortical neurons |
DOI: 10.3390/nu12113511 |
|
PMID: 33202660 |
Sample: Serum |
DOI: 10.1002/biof.1692 |
|
Sample: serum |
PMID: 34871743 |
Sample: hippocampus,liver |
DOI: 10.2147/DMSO.S214726 |
|
PMID: 34707382 |
Sample: plasma |
PMID: 34499695 |
Sample: serum,Lung tissue |
Sample: Tissue homogenate |
DOI: 10.2147/DMSO.S218626 |
|
Sample: plasma |
PMID: 35058758 |
Sample: hippocampus tissue |
PMID: 35078449 |
Sample: serum |
Sample: plasma,kidney tissues |
PMID: 31491333 |
Sample: Serum,Plasma,Tissue homogenate |
PMID: 35918636 |
Sample: heart tissue |
Sample: kidney |
PMID: 32594358 |
Sample: Tissue homogenate |
DOI: 10.1155/2022/7832540 |
|
Sample: hepatic tissue |
Sample: kidney |
DOI: 10.1002/jemt.23798 |
|
PMID: 33908126 |
Sample: Tissue homogenate(liver) |
PMID: 32489292 |
Sample: Serum |
Sample: hippocampus |
Sample: plasma |
Sample: Serum |
Sample: Tissue homogenate |
Sample: Serum |
PMID: 35507975 |
Sample: Plasma,vastus intermedius muscle tissue |
PMID: 35331657 |
Sample: serum,cochlea |
Sample: Serum,Urine,Tissue homogenate |
Sample: Tissue homogenate |
E********bSubmitted [ Dec 17 2020 ]
Asked: What species can Catalog.No: E-EL-R1424 be used? Rat, mouse? thank you
Reply
adminSubmitted [ Dec 17 2020 ]
Answered: Hi Elly, Thanks for your message. E-EL-R1424 detects Rat samples