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Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Citations(35)Uniprot : P07632

Cat.No.:E-EL-R1424
Reactivity: Rat

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.20	3.40	0.50	1.30	3.60
Standard deviation	0.03	0.07	0.12	0.03	0.07	0.13
CV (%)	6.00	5.83	3.53	6.00	5.38	3.61

Recovery

The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-102	97
EDTA plasma(n=8)	91-104	97
Cell culture media(n=8)	89-105	96

Linearity

Samples were spiked with high concentrations of Rat SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P07632
Synonyms	ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]
Research Area	Cancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Biomedicines (2021) IF: 6.081

WIN55,212-2 Attenuates Cognitive Impairments in AlCl3 + d-Galactose-Induced Alzheimer’s Disease Rats by Enhancing Neurogenesis and Reversing Oxidative Stress

DOI: 10.3390/biomedicines9091270
	Sample: brain

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53

Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress

DOI: 10.1016/j.biopha.2022.113225
PMID: 35671584	Sample: serum

INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY (2019) IF: 5.176

Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats

DOI: 10.1093/ijnp/pyy090
	Sample: Plasma,Tissue homogenate

RNA Biology (2019) IF: 5.477

MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

DOI: 10.1080/15476286.2019.1657352
PMID: 31425004	Sample: Serum,Cell culture medium

Frontiers in Pharmacology (2021) IF: 5.811

The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702
	Sample: serum

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223

Centella asiatica extract protects against cisplatin-induced hepatotoxicity via targeting oxidative stress, inflammation, and apoptosis

DOI: 10.1007/s11356-022-18626-z
PMID: 35029831	Sample: hepatic tissue

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486

Protection of melatonin against acidosis‐induced neuronal injuries

DOI: 10.1111/jcmm.15351
	Sample: Primary cortical neurons

Nutrients (2020) IF: 4.546

Effect of Defatted Dabai Pulp Extract in Urine Metabolomics of Hypercholesterolemic Rats

DOI: 10.3390/nu12113511
PMID: 33202660	Sample: Serum

BIOFACTORS (2020) IF: 4.734

Berberine promotes peri‐implant osteogenesis in diabetic rats by ROS‐mediated IRS‐1 pathway

DOI: 10.1002/biof.1692
	Sample: serum

NEUROSCIENCE LETTERS (2021) IF: 3.046

Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385
PMID: 34871743	Sample: hippocampus,liver

Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168

Heamanetic Effects of a Dioxidovanadium(V) Complex in STZ-Induced Diabetic Male Sprague Dawley Rats

DOI: 10.2147/DMSO.S214726
PMID: 34707382	Sample: plasma

PLoS One (2021) IF: 3.24

A low direct electrical signal attenuates oxidative stress and inflammation in septic rats

DOI: 10.1371/journal.pone.0257177
PMID: 34499695	Sample: serum,Lung tissue

TOXICOLOGY (2018) IF: 3.265

Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings

DOI: 10.1016/j.tox.2018.05.012
	Sample: Tissue homogenate

Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2019) IF: 3.319

Plant-derived oleanolic acid ameliorates markers associated with non-alcoholic fatty liver disease in a diet-induced pre-diabetes rat model

DOI: 10.2147/DMSO.S218626
	Sample: plasma

Frontiers in Behavioral Neuroscience (2022) IF: 3.558

Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats

DOI: 10.3389/fnbeh.2021.783056
PMID: 35058758	Sample: hippocampus tissue

BMC Complementary and Alternative Medicine (2022) IF: 3.659

Attenuation of carbohydrate metabolism and lipid profile by methanolic extract of Euphorbia helioscopia and improvement of beta cell function in a type 2 diabetic rat model

DOI: 10.1186/s12906-022-03507-2
PMID: 35078449	Sample: serum

BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743

The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers

DOI: 10.1016/j.biopha.2019.108925
	Sample: plasma,kidney tissues

NEUROCHEMISTRY INTERNATIONAL (2021) IF: 3.921

Exposure to footshock stress downregulates antioxidant genes and increases neuronal apoptosis in an Aβ(1–42) rat model of Alzheimer's disease

DOI: 10.1016/j.neuint.2021.105170

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019) IF: 2.041

The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

DOI: 10.1139/cjpp-2019-0369
PMID: 31491333	Sample: Serum,Plasma,Tissue homogenate

BMC Cardiovascular Disorders (2022) IF: 2.174

Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

DOI: 10.1186/s12872-022-02758-8
PMID: 35918636	Sample: heart tissue

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2021) IF: 2.273

Anti-hyperglycaemic effects of dioxidovanadium complex cis-[VO2(obz)py] avert kidney dysfunction in streptozotocin-induced diabetic male Sprague–Dawley rats

DOI: 10.1139/cjpp-2020-0278
	Sample: kidney

BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639

Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods

DOI: 10.1007/s12011-020-02249-x
PMID: 32594358	Sample: Tissue homogenate

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65

San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540
	Sample: hepatic tissue

Dose-Response (2022) IF: 2.658

Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study:

DOI: 10.1177/15593258211073087
	Sample: kidney

MICROSCOPY RESEARCH AND TECHNIQUE (2021) IF: 2.769

Histological, immunohistochemical, and molecular investigation on the hepatotoxic effect of potassium dichromate and the ameliorating role of Persea americana mill pulp extract

DOI: 10.1002/jemt.23798
PMID: 33908126	Sample: Tissue homogenate(liver)

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2020) IF: 2.802

Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats

DOI: 10.1016/j.sjbs.2020.03.009
PMID: 32489292	Sample: Serum

BRAIN RESEARCH (2019) IF: 2.929

Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus

DOI: 10.1016/j.brainres.2019.146326
	Sample: hippocampus

Molecular Medicine Reports (2018) IF: 2.952

Puerarin reduces ischemia/reperfusion-induced myocardial injury in diabetic rats via upregulation of vascular endothelial growth factor A/angiotensin-1 and suppression of apoptosis

DOI: 10.3892/mmr.2018.8754
	Sample: plasma

Experimental and Therapeutic Medicine (2017) IF: 1.261

Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β

DOI: 10.3892/etm.2017.4505
	Sample: Serum

Anatolian Journal of Cardiology (2018) IF: 1.271

Protective effects of curcumin and beta-carotene on cisplatin-induced cardiotoxicity: An experimental rat model

DOI: 10.14744/AnatolJCardiol.2018.53059
	Sample: Tissue homogenate

Molecular Medicine Reports (2015) IF: 1.554

Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti‑inflammatory effects, and inhibition of the p38‑MAPK mechanism

DOI: 10.3892/mmr.2015.4425
	Sample: Serum

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2022) IF: 1.753

Protective effect by low-intensity downhill running training against muscle damage and oxidative stress after high-intensity downhill running in rats

DOI: 10.1590/0001-3765202220200265
PMID: 35507975	Sample: Plasma,vastus intermedius muscle tissue

Brazilian Journal of Otorhinolaryngology (2022) IF: 1.811

Astaxanthin protects against hearing impairment in diabetic rats

DOI: 10.1016/j.bjorl.2022.02.004
PMID: 35331657	Sample: serum,cochlea

INTERNATIONAL UROLOGY AND NEPHROLOGY (2020) IF: 1.843

Vinpocetine reduces cisplatin-induced acute kidney injury through inhibition of NF–κB pathway and activation of Nrf2/ARE pathway in rats

DOI: 10.1007/s11255-020-02485-z
	Sample: Serum,Urine,Tissue homogenate

Advances in Traditional Medicine (2020)

Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus

DOI: 10.1007/s13596-020-00449-3
	Sample: Tissue homogenate

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Reviews/Q&A

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Reviews
Q&A

E********bSubmitted [ Dec 17 2020 ]

Asked: What species can Catalog.No: E-EL-R1424 be used? Rat, mouse? thank you

adminSubmitted [ Dec 17 2020 ]

Answered: Hi Elly, Thanks for your message. E-EL-R1424 detects Rat samples

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