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Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

  • Cat.No.:E-EL-R1424

  • Reactivity: Rat

To Purchase E-EL-R1424

Size:
  • 96T
  • 48T
  • 24T
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.20 3.40 0.50 1.30 3.60
Standard deviation 0.03 0.07 0.12 0.03 0.07 0.13
CV (%) 6.00 5.83 3.53 6.00 5.38 3.61

Recovery

The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma(n=8) 91-104 97
Cell culture media(n=8) 89-105 96

Linearity

Samples were spiked with high concentrations of Rat SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P07632
SynonymsALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]
Research AreaCancer,Cell biology

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. RNA biology (2019) IF: 5.35
    MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

    DOI: 10.1080/15476286.2019.1657352

    PMID: 31425004

    Sample: Serum,Cell culture medium
  2. International Journal of Nanomedicine (2018) IF: 4.37
    Tanshinol borneol ester on nanostructured lipid carriers has longer brain and systemic effector retention and better antioxidant activity in vivo.

    DOI: 10.2147/IJN.S159789

    PMID: 29695905

    Sample: Tissue homogenate
  3. International Journal of Neuropsychopharmacology (2018) IF: 3.981
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    Sample: Plasma,Tissue homogenate
  4. International Journal of Neuropsychopharmacology IF: 3.98
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    Sample: Serum, Tissue homogenate
  5. Toxicology (2018) IF: 3.265
    Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings.

    DOI: 10.1016/j.tox.2018.05.012

    PMID: 29800585

    Sample: Tissue homogenate
  6. Anatolian Journal of Cardiology (2018) IF: 3.767
    Protective Effects of Curcumin and Beta-carotene on Cisplatin-induced Cardiotoxicity: An Experimental Rat Model.

    DOI: 10.14744/AnatolJCardiol.2018.53059

    PMID: 29521316

  7. Biological Trace Element Research (2020) IF: 2.639
    Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods

    DOI: 10.1007/s12011-020-02249-x

    PMID: 32594358

    Sample: Tissue homogenate
  8. Saudi Journal of Biological Sciences (2020) IF: 2.802
    Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats

    DOI: 10.1016/j.sjbs.2020.03.009

    PMID: 32489292

    Sample: Serum
  9. International urology and nephrology (2020) IF: 1.843
    Vinpocetine reduces cisplatin-induced acute kidney injury through inhibition of NF-κB pathway and activation of Nrf2/ARE pathway in rats

    DOI: 10.1007/s11255-020-02485-z

    PMID: 32418008

    Sample: Serum,Urine,Tissue homogenate
  10. Canadian journal of physiology and pharmacology (2019) IF: 1.946
    The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2019-0369

    PMID: 31491333

    Sample: Serum,Plasma,Tissue homogenate
  11. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Tocotrienol Alleviates Inflammation And Oxidative Stress In A Rat Model Of Spinal Cord Injury Via Suppression Of Transforming Growth Factor-β.

    DOI: 10.3892/etm.2017.4505

    PMID: 28672950

    Sample: Serum
  12. Molecular Medicine Reports (2015) IF: 1.692
    Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism.

    DOI: 10.3892/mmr.2015.4425

    PMID: 26458544

    Sample: Serum
  13. Oriental Pharmacy and Experimental Medicine (2020)
    Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus

    DOI: 10.1007/s13596-020-00449-3

    PMID: 99999999

    Sample: Tissue homogenate
  14. Annals of Tropical Medicine & Public Health (2019)
    Protective effect of Melatonin, Rosuvastatin and their combination against Amikacin induced nephrotoxicity in rats

    DOI: 10.36295/ASRO.2019.220513

    PMID: 99999999

    Sample: Serum
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

E********bSubmitted [ Dec 17 2020 ]

Asked: What species can Catalog.No: E-EL-R1424 be used? Rat, mouse? thank you

Reply

A

adminSubmitted [ Dec 17 2020 ]

Answered: Hi Elly, Thanks for your message. E-EL-R1424 detects Rat samples

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