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Rat TSH(Thyroid Stimulating Hormone) ELISA Kit

Cat.No.:E-EL-R0976
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.25-80 ng/mL
Sensitivity	0.75 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat TSH in samples. No significant cross-reactivity or interference between Rat TSH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat TSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TSH. You can calculate the concentration of Rat TSH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TSH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.20	7.90	38.10	4.60	7.50	38.10
Standard deviation	0.30	0.30	1.30	0.30	0.40	1.60
CV (%)	7.14	3.80	3.41	6.52	5.33	4.20

Recovery

The recovery of Rat TSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-105	98
EDTA plasma (n=8)	93-108	100
Cell culture media (n=8)	88-100	94

Linearity

Samples were spiked with high concentrations of Rat TSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Thyrotropin
Research Area	Cancer, Metabolism, Neuroscience, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CHEMOSPHERE (2021) IF: 7.086
Adverse postnatal developmental effects in offspring from humidifier disinfectant biocide inhaled pregnant rats

DOI: 10.1016/j.chemosphere.2021.131636

Sample: Serum
SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats

DOI: 10.1016/j.scitotenv.2021.145196

PMID: 34030373
Sample: Serum
Computational and Structural Biotechnology Journal (2020) IF: 6.018
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

DOI: 10.1016/j.csbj.2020.11.009

PMID: 33304464
Sample: serum
JOURNAL OF MOLECULAR ENDOCRINOLOGY (2021) IF: 5.098
Pathological changes and oxidative stress of the HPG axis in hypothyroid rat

DOI: 10.1530/JME-21-0095

PMID: 34270447
Sample: serum
BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring

DOI: 10.1016/j.bbr.2022.114082

PMID: 36041571
Sample: serum
Biomed Research International (2021) IF: 3.411
Effect of Preeclampsia on Ultrastructure of Thyroid Gland, Hepatic Type 1 Iodothyronine Deiodinase, and Thyroid Hormone Levels in Rats

DOI: 10.1155/2021/6681491

PMID: 34195279
Sample: Serum
BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
The Protective effect of P7C3 against DNA and neuron damage in rat pups with congenital hypothyroidism

DOI: 10.1016/j.biopha.2018.01.058

Sample: plasma
DRUG METABOLISM AND DISPOSITION (2018) IF: 3.64
Activation of 5-HT1A Receptors in the Hypothalamic Paraventricular Nuclei Negatively Regulates Cytochrome P450 Expression and Activity in Rat Liver

DOI: 10.1124/dmd.117.079632

Sample: Serum,Tissue homogenate
BMC Complementary and Alternative Medicine (2022) IF: 3.659
Attenuation of carbohydrate metabolism and lipid profile by methanolic extract of Euphorbia helioscopia and improvement of beta cell function in a type 2 diabetic rat model

DOI: 10.1186/s12906-022-03507-2

PMID: 35078449
Sample: serum
BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 3.738
The Effect of Irisin on Thyroid Hormone Levels in Chronic Paroxetine-Treated Rats

DOI: 10.1007/s12011-022-03204-8

PMID: 35322355
Sample: serum
INTERNATIONAL JOURNAL OF TOXICOLOGY (2021) IF: 2.032
Palmitoylethanolamide: Prenatal Developmental Toxicity Study in Rats:

DOI: 10.1177/1091581820986073

Sample: Serum
International Journal of Endocrinology (2019) IF: 2.287
Aliskiren, Fosinopril, and Their Outcome on Renin-Angiotensin-Aldosterone System (RAAS) in Rats with Thyroid Dysfunction

DOI: 10.1155/2019/5960563

Sample: serum
JOURNAL OF PHARMACY AND PHARMACOLOGY (2019) IF: 2.39
The effect of eplerenone on the renin‐angiotensin‐aldosterone system of rats with thyroid dysfunction

DOI: 10.1111/jphp.13168

Sample: Serum
Journal of Physiological Sciences (2018) IF: 2.757
Effects of central FGF21 infusion on the hypothalamus–pituitary–thyroid axis and energy metabolism in rats

DOI: 10.1007/s12576-018-0595-7

Sample: serum
JOURNAL OF INVESTIGATIVE SURGERY (2017) IF: 1.532
Effects of Thyroid Hormone Therapy on Cut-Surface Healing of the Remnant Stomach with Short-Term Weight Loss Alterations after Sleeve Gastrectomy

DOI: 10.1080/08941939.2017.1280566

Sample: Serum,Tissue homogenate
JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
Effect of myrtle (Myrtus communis L.) essential oil on oxidant–antioxidant balance in rats with propylthiouracil-induced hypothyroidism

DOI: 10.1111/jfbc.13498

Sample: Serum
Cureus (2021)
The Interplay Between Hyperthyroidism and Ovarian Cytoarchitecture in Albino Rats

DOI: 10.7759/cureus.14517

Sample: serum
Hormone Molecular Biology and Clinical Investigation (2021)
Effect of short-term and long-term traffic noise exposure on the thyroid gland in adult rats: a sexual dimorphic study

DOI: 10.1515/hmbci-2020-0029

Sample: plasma

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