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Rat VCAM-1/CD106(Vascular Cell Adhesion Molecule 1) ELISA Kit

Cat.No.:E-EL-R1061
Reactivity: Rat

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	12.50-800 pg/mL
Sensitivity	7.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Rat VCAM-1/CD106 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. You can calculate the concentration of Rat VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VCAM-1/CD106 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VCAM-1/CD106 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	39.80	71.20	345.30	42.10	77.80	375.50
Standard deviation	2.50	3.20	15.90	2.70	3.50	13.50
CV (%)	6.28	4.49	4.60	6.41	4.50	3.60

Recovery

The recovery of Rat VCAM-1/CD106 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-108	100
EDTA plasma (n=8)	85-101	92
Cell culture media (n=8)	85-96	91

Linearity

Samples were spiked with high concentrations of Rat VCAM-1/CD106 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CD106, INCAM-100
Research Area	Cancer, Cardiovascular, Neuroscience, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ANNALS OF THE RHEUMATIC DISEASES (2022) IF: 19.103
POS0483 THE EFFECT OF VITAMIN D3 AND α-TOCOPHEROL ACETATE IN THE PRECLINICAL MODEL OF SYSTEMIC SCLEROSIS

DOI: 10.1136/annrheumdis-2022-eular.3061

Sample: serum
BRITISH JOURNAL OF ANAESTHESIA (2021) IF: 9.166
Bosutinib reduces endothelial permeability and organ failure in a rat polytrauma transfusion model

DOI: 10.1016/j.bja.2021.01.032

PMID: 33685634
Sample: Whole Blood
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Hepatocyte Nuclear Factor 1α Proinflammatory Effect Linked to the Overexpression of Liver Nuclear Factor–κB in Experimental Model of Chronic Kidney Disease

DOI: 10.3390/ijms23168883

Sample: serum
Scientific Reports (2016) IF: 5.228
Endothelial dysfunction correlates with decompression bubbles in rats

DOI: 10.1038/srep33390

Sample: Serum
REDOX REPORT (2022) IF: 5.696
Atorvastatin-mediated downregulation of VCAM-1 and XO/UA/caspase 3 signaling averts oxidative damage and apoptosis induced by ovarian ischaemia/reperfusion injury

DOI: 10.1080/13510002.2022.2129192

PMID: 36200598
Sample: ovarian
Frontiers in Physiology (2017) IF: 4.134
Implication of Free Fatty Acids in Thrombin Generation and Fibrinolysis in Vascular Inflammation in Zucker Rats and Evolution with Aging

DOI: 10.3389/fphys.2017.00949

Sample: vascular smooth muscle cell
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Pleiotropic vascular effects of ivabradine in streptozotocin-induced diabetes

DOI: 10.1016/j.ejphar.2021.174551

PMID: 34906548
Sample: plasma
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Diacerein protects rats with liver ischemia/reperfusion damage: Down-regulation of TLR4/ NFκ-B signaling pathway

DOI: 10.1016/j.biopha.2020.111063

PMID: 33348310
Sample: Tissue homogenate
EXPERIMENTAL BIOLOGY AND MEDICINE (2020) IF: 3.139
H2S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty:

DOI: 10.1177/1535370220961038

Sample: Plasma
EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

DOI: 10.1016/j.ejphar.2019.172786

PMID: 31712060
Sample: Serum
Frontiers in Physiology (2018) IF: 3.394
Age-, Gender-, and in Vivo Different Doses of Isoproterenol Modify in Vitro Aortic Vasoreactivity and Circulating VCAM-1

DOI: 10.3389/fphys.2018.00020

Sample: plasma
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
Possible mechanisms mediating the protective effect of cilostazol in L-arginine induced acute pancreatitis in rats: role of cGMP, cAMP, and HO-1

DOI: 10.1007/s00210-020-01897-z

PMID: 32424476
Sample: Serum
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2019) IF: 2.058
Mineralocorticoid receptor blockade attenuates disrupted glutathione-dependent antioxidant defense and elevated endoglin in the hearts of pregnant rats exposed to testosterone

DOI: 10.1007/s00210-019-01630-5

PMID: 30783718
Sample: Plasma
HUMAN & EXPERIMENTAL TOXICOLOGY (2020) IF: 2.067
Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats:

DOI: 10.1177/0960327120960775

Sample: Tissue homogenate
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2019) IF: 2.11
Late gestational testosterone exposure causes glucose deregulation and elevated cardiac VCAM-1 and DPP-4 activity in rats

DOI: 10.1080/13813455.2019.1650068

PMID: 31387411
Sample: Plasma
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 1.806
Glucocorticoid exposure causes disrupted glucoregulation, cardiac inflammation and elevated dipeptidyl peptidase-4 activity independent of glycogen synthase kinase-3 in female rats

DOI: 10.1080/13813455.2018.1479426

Sample: Plasma
ANDROLOGIA (2019) IF: 1.84
Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM‐1 and VEGF‐A

DOI: 10.1111/and.13472

Sample: Serum
Reproductive Biology (2020) IF: 1.862
Leptin-induced increase in blood pressure and markers of endothelial activation during pregnancy in Sprague Dawley rats is prevented by resibufogenin, a marinobufagenin antagonist

DOI: 10.1016/j.repbio.2020.03.004

PMID: 32253169
Sample: Serum
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2020) IF: 1.946
Dexamethasone causes defective glucose-6-phosphate dehydrogenase dependent antioxidant barrier through endoglin in pregnant and nonpregnant rats

DOI: 10.1139/cjpp-2018-0351

Sample: Plasma,Tissue homogenate

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