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Rat VCAM-1/CD106(Vascular Cell Adhesion Molecule 1) ELISA Kit

  • Cat.No.:E-EL-R1061

  • Reactivity: Rat

To Purchase E-EL-R1061

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 12.50-800 pg/mL
Sensitivity 7.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Rat VCAM-1/CD106 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. You can calculate the concentration of Rat VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VCAM-1/CD106 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VCAM-1/CD106 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 39.80 71.20 345.30 42.10 77.80 375.50
Standard deviation 2.50 3.20 15.90 2.70 3.50 13.50
CV (%) 6.28 4.49 4.60 6.41 4.50 3.60

Recovery

The recovery of Rat VCAM-1/CD106 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-108 100
EDTA plasma (n=8) 85-101 92
Cell culture media (n=8) 85-96 91

Linearity

Samples were spiked with high concentrations of Rat VCAM-1/CD106 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCD106, INCAM-100
Research AreaCancer, Cardiovascular, Neuroscience, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. British Journal Of Anaesthesia (2021) IF: 9.166
    Bosutinib reduces endothelial permeability and organ failure in a rat polytrauma transfusion model

    DOI: 10.1016/j.bja.2021.01.032

    PMID: 33685634

    Sample: Whole Blood
  2. Scientific Reports (2016) IF: 4.259
    Endothelial Dysfunction Correlates With Decompression Bubbles In Rats.

    DOI: 10.1038/srep33390

    PMID: 27615160

    Sample: Serum
  3. Experimental Biology and Medicine (2020) IF: 3.139
    H 2 S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty

    DOI: 10.1177/1535370220961038

    PMID: 32996350

    Sample: Plasma
  4. European Journal of Pharmacology (2019) IF: 3.263
    Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

    DOI: 10.1016/j.ejphar.2019.172786

    PMID: 31712060

    Sample: Serum
  5. Human & Experimental Toxicology (2020) IF: 2.067
    Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats

    DOI: 10.1177/0960327120960775

    PMID: 32959702

    Sample: Tissue homogenate
  6. Archives of Physiology and Biochemistry (2019) IF: 2.575
    Late gestational testosterone exposure causes glucose deregulation and elevated cardiac VCAM-1 and DPP-4 activity in rats

    DOI: 10.1080/13813455.2019.1650068

    PMID: 31387411

    Sample: Plasma
  7. Naunyn-Schmiedeberg's Archives of Pharmacology (2020) IF: 2.05
    Possible mechanisms mediating the protective effect of cilostazol in L-arginine induced acute pancreatitis in rats: role of cGMP, cAMP, and HO-1

    DOI: 10.1007/s00210-020-01897-z

    PMID: 32424476

    Sample: Serum
  8. Reproductive Biology (2020) IF: 1.862
    Leptin-induced increase in blood pressure and markers of endothelial activation during pregnancy in Sprague Dawley rats is prevented by resibufogenin, a marinobufagenin antagonist

    DOI: 10.1016/j.repbio.2020.03.004

    PMID: 32253169

    Sample: Serum
  9. Canadian Journal of Physiology and Pharmacology (2020) IF: 1.946
    Dexamethasone causes defective glucose-6-phosphate dehydrogenase-dependent antioxidant barrier through endoglin in pregnant and non-pregnant rats

    DOI: 10.1139/cjpp-2018-0351

    PMID: 32259461

    Sample: Plasma,Tissue homogenate
  10. Andrologia (2020) IF: 1.951
    Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM-1 and VEGF-A

    DOI: 10.1111/and.13472

    PMID: 31773790

    Sample: Serum
  11. Archives of physiology and biochemistry (2018) IF: 1.806
    Glucocorticoid exposure causes disrupted glucoregulation, cardiac inflammation and elevated dipeptidyl peptidase-4 activity independent of glycogen synthase kinase-3 in female rats.

    DOI: 10.1080/13813455.2018.1479426

    PMID: 29912577

    Sample: Plasma
  12. Naunyn-Schmiedeberg's archives of pharmacology (2019)
    Mineralocorticoid receptor blockade attenuates disrupted glutathione-dependent antioxidant defense and elevated endoglin in the hearts of pregnant rats exposed to testosterone

    DOI: 10.1007/s00210-019-01630-5

    PMID: 30783718

    Sample: Plasma
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