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Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

Cat.No.:E-EL-R2603
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VEGF-A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	97.90	170.39	948.99	89.62	158.20	968.59
Standard deviation	5.02	9.25	46.60	5.65	7.26	37.97
CV (%)	5.13	5.43	4.91	6.30	4.59	3.92

Recovery

The recovery of Rat VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-101	93
EDTA plasma (n=8)	90-102	95
Cell culture media (n=8)	89-101	95

Linearity

Samples were spiked with high concentrations of Rat VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Bioactive Materials (2021) IF: 14.593

Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

DOI: 10.1016/j.bioactmat.2021.03.020
PMID: 33842738	Sample: Cell culture supernatant

Stem Cell Research & Therapy (2020) IF: 5.116

Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model

DOI: 10.1186/s13287-020-01733-4
PMID: 32456711	Sample: Plasma

RNA biology (2019) IF: 5.35

MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

DOI: 10.1080/15476286.2019.1657352
PMID: 31425004	Sample: Serum,Cell culture medium

International Journal of Nanomedicine (2019) IF: 4.471

Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

DOI: 10.2147/IJN.S184920
PMID: 30666115	Sample: Cell culture supernatant

J Cell Sci (2018) IF: 4.401

Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.

DOI: 10.1242/jcs.216135
PMID: 29898921	Sample: Tissue homogenate

Archives of biochemistry and biophysics (2019) IF: 3.391

Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture

DOI: 10.1016/j.abb.2019.108108
PMID: 31550444	Sample: Cell lysate

Colloids and Surfaces B: Biointerfaces (2018) IF: 3.997

The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models.

DOI: 10.1016/j.colsurfb.2018.04.060
PMID: 29730577	Sample: VEGF solution after immobilization

Human & Experimental Toxicology (2020) IF: 2.067

Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats

DOI: 10.1177/0960327120960775
PMID: 32959702	Sample: Tissue homogenate

Human & Experimental Toxicology (2020) IF: 2.067

PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats

DOI: 10.1177/0960327120914977
PMID: 32228192	Sample: Tissue homogenate

Andrologia (2020) IF: 1.951

Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM-1 and VEGF-A

DOI: 10.1111/and.13472
PMID: 31773790	Sample: Serum

Universitas Airlangga (2018)

PENGARUH CURCUMIN TERHADAP KADAR VEGF VITREORETINA PADA MODEL TIKUS HIPERGLIKEMIA (Studi Eksperimental pada Tikus Wistar)


	Sample: vitreo

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