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Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

  • Cat.No.:E-EL-R2603

  • Reactivity: Rat

To Purchase E-EL-R2603

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VEGF-A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 97.90 170.39 948.99 89.62 158.20 968.59
Standard deviation 5.02 9.25 46.60 5.65 7.26 37.97
CV (%) 5.13 5.43 4.91 6.30 4.59 3.92


The recovery of Rat VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-101 93
EDTA plasma (n=8) 90-102 95
Cell culture media (n=8) 89-101 95


Samples were spiked with high concentrations of Rat VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Bioactive Materials (2021) IF: 14.593
    Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair

    DOI: 10.1016/j.bioactmat.2021.03.020

    PMID: 33842738

    Sample: Cell culture supernatant
  2. Stem Cell Research & Therapy (2020) IF: 5.116
    Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model

    DOI: 10.1186/s13287-020-01733-4

    PMID: 32456711

    Sample: Plasma
  3. RNA biology (2019) IF: 5.35
    MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

    DOI: 10.1080/15476286.2019.1657352

    PMID: 31425004

    Sample: Serum,Cell culture medium
  4. International Journal of Nanomedicine (2019) IF: 4.471
    Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

    DOI: 10.2147/IJN.S184920

    PMID: 30666115

    Sample: Cell culture supernatant
  5. J Cell Sci (2018) IF: 4.401
    Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.

    DOI: 10.1242/jcs.216135

    PMID: 29898921

    Sample: Tissue homogenate
  6. Archives of biochemistry and biophysics (2019) IF: 3.391
    Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture

    DOI: 10.1016/

    PMID: 31550444

    Sample: Cell lysate
  7. Colloids and Surfaces B: Biointerfaces (2018) IF: 3.997
    The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models.

    DOI: 10.1016/j.colsurfb.2018.04.060

    PMID: 29730577

    Sample: VEGF solution after immobilization
  8. Human & Experimental Toxicology (2020) IF: 2.067
    Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats

    DOI: 10.1177/0960327120960775

    PMID: 32959702

    Sample: Tissue homogenate
  9. Human & Experimental Toxicology (2020) IF: 2.067
    PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats

    DOI: 10.1177/0960327120914977

    PMID: 32228192

    Sample: Tissue homogenate
  10. Andrologia (2020) IF: 1.951
    Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM-1 and VEGF-A

    DOI: 10.1111/and.13472

    PMID: 31773790

    Sample: Serum
  11. Universitas Airlangga (2018)
    Sample: vitreo
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