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SARS-CoV-2 Spike Protein IgG ELISA Kit

  • Cat.No.:E-EL-E602

  • Reactivity: Human
  • Assay Type: Indirect-ELISA
  • Assay time: 1.5h

To Purchase E-EL-E602

Size:
  • 96T
  • 24T
  • 96T*5
  • 96T*10
Price: $500
Qty:

Properties

Format 24T, 96T, 96T*5, 96T*10
Assay time 1.5h
Sample type Human serum or plasma
Sample volume 100 μL
Storage 2-8℃
Interpretation Qualitative
Application This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 IgG in serum or plasma.
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms COVID 19 IgG,S protein IgG

Test Principle

This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgG in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgG in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgG to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgG and HRP conjugated anti-human IgG will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgG exists in the tested samples or not.

Assay Procedures

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