Intended
use
This
ELISA kit applies to the in vitro qualitative determination of SARS-CoV-2 Spike Protein IgG
in serum or plasma from pneumonia
patients infected with SARS-CoV-2.
Test
principle
This
ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 S pike protein IgG in the sample. The
micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgG in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP)
conjugated mouse anti human IgG to develop the antigen-antibody-HRP conjugated secondary
antibody complex. Free components are washed away, then the substrate solution
is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgG and
HRP conjugated anti-human IgG will appear blue in color. The enzyme-substrate
reaction is terminated by the addition of stop solution and the color turns
yellow. The optical density (OD) is measured spectrophotometrically at a
wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgG
exists in the tested samples or not.
Kit components
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the
reagents according to the conditions below.
|
Item
|
Specifications
|
Storage
|
|
Micro ELISA Plate(Dismountable)
|
8 wells ×12 strips
|
2-8℃, 6 months
|
|
Positive Control
|
2 vials
|
2-8℃, 6 months
|
|
Negative Control
|
2 vials
|
|
Sample &
Control Diluent
|
1
vial, 25 mL
|
|
Concentrated HRP Conjugated Mouse anti-human
IgG（100×）
|
1 vial, 120 μL
|
2-8℃(Protect from light), 6 months
|
|
HRP
Conjugate Diluent
|
1 vial, 14 mL
|
2-8℃,6
months
|
|
Concentrated
Wash Buffer（25×）
|
1
vial, 30 mL
|
|
Stop Solution
|
1
vial, 10 mL
|
|
SubstrateReagent
|
1
vial, 10 mL
|
2-8℃(Protect from light), 6 months
|
|
Plate Sealer
|
5
pieces
|
|
|
Product Description
|
1
copy
|
|
Certificate of
Analysis
|
1
copy
|
Note:All reagent bottle caps must be tightened to prevent evaporation and
microbial pollution. The volume of reagents in partial shipments is a little
more than the volume marked on the label, please use accurate measuring
equipment instead of directly pouring into the vial(s).
Other supplies required
Microplate Reader with 450 nm wavelength filter or
dual-wavelength (450/630 nm)
High-precision transfer pettor, EP tubes and disposable
pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash
Buffer
Performance
Intra-CV:CV%＜8%
3 samples with low, mid-range and high level were tested 20 times on
one plate respectively.
Inter-CV:CV%＜10%
3
samples with low, mid-range and high level were tested on 3 different plates,
20 replicates in each plate.
Message submitted successfully !