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Catalog number:E-EL-E602
Synonyms:COVID 19 IgG,S protein IgG
Reactivity: Human
Assaytype: Indirect-ELISA
Assaytime: 1.5h
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This ELISA kit applies to the in vitro qualitative determination of SARS-CoV-2 Spike Protein IgG in serum or plasma from pneumonia patients infected with SARS-CoV-2.
Test principle
This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 S pike protein IgG in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgG in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgG to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgG and HRP conjugated anti-human IgG will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgG exists in the tested samples or not.
Kit components
The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the reagents according to the conditions below.
Item |
Specifications |
Storage |
Micro ELISA Plate(Dismountable) |
8 wells ×12 strips |
2-8℃, 6 months |
Positive Control |
2 vials |
2-8℃, 6 months |
Negative Control |
2 vials |
|
Sample & Control Diluent |
1 vial, 25 mL |
|
Concentrated HRP Conjugated Mouse anti-human IgG(100×) |
1 vial, 120 μL |
2-8℃(Protect from light), 6 months |
HRP Conjugate Diluent |
1 vial, 14 mL |
2-8℃,6 months |
Concentrated Wash Buffer(25×) |
1 vial, 30 mL |
|
Stop Solution |
1 vial, 10 mL |
|
SubstrateReagent |
1 vial, 10 mL |
2-8℃(Protect from light), 6 months |
Plate Sealer |
5 pieces |
|
Product Description |
1 copy |
|
Certificate of Analysis |
1 copy |
Note:All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).
Other supplies required
Microplate Reader with 450 nm wavelength filter or dual-wavelength (450/630 nm)
High-precision transfer pettor, EP tubes and disposable pipette tips
Incubator capable of maintaining 37℃
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
Performance
Intra-CV:CV%<8%
3 samples with low, mid-range and high level were tested 20 times on one plate respectively.
Inter-CV:CV%<10%
3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate.