SARS-CoV-2 Spike Protein IgM ELISA Kit

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    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-E603

      Synonyms:COVID 19 IgM,S protein IgM

      Size:
      • 96T
      Qty:
      - +
      Price: $500

      Reactivity: Human

      Assaytype: Indirect-ELISA

      Assaytime: 1.5h

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro qualitative determination of SARS-CoV-2 Spike Protein IgM in serum or plasma from pneumonia patients infected with SARS-CoV-2.

       

      Test principle

      This ELISA kit uses Indirect-ELISA as the method to qualitatively detect the SARS-CoV-2 Spike protein IgM in the sample. The micro ELISA plate provided in this kit is pre-coated with purified SARS-CoV-2 Spike protein (PKSR030489) antigen, after adding samples to wells, the SARS-CoV-2 Spike protein IgM in the samples will combine with the pre-coated SARS-CoV-2 Spike protein antigen. After washing completely, add Horseradish Peroxidase (HRP) conjugated mouse anti human IgM to develop the antigen-antibody-HRP conjugated secondary antibody complex. Free components are washed away, then the substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike protein IgM and HRP conjugated anti-human IgM will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike protein IgM exists in the tested samples or not.

       

      Kit components

      The unopened kit can be stable for 6 months at 2-8℃. After opening the kit, keep the reagents according to the conditions below.

      Item

      Specifications

      Storage

      Micro ELISA Plate(Dismountable)

      8 wells ×12 strips

      2-8℃, 6 months

      Positive Control

      2 vials

      2-8℃, 6 months

      Negative Control

      2 vials

      Sample & Control Diluent

      1 vial, 25 mL

      Concentrated HRP Conjugated Mouse anti-human IgM(100×)

      1 vial, 120 μL

      2-8℃(Protect from light), 6 months

      HRP Conjugate Diluent

      1 vial, 14 mL

      2-8℃,6 months

      Concentrated Wash Buffer(25×)

      1 vial, 30 mL

      Stop Solution

      1 vial, 10 mL

      SubstrateReagent

      1vial, 10 mL

      2-8℃(Protect from light), 6 months

      Plate Sealer

      5 pieces

       

      Product Description

      1 copy

      Certificate of Analysis

      1 copy

      Note:All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

       

      Other supplies required

      Microplate Reader with 450 nm wavelength filter or dual-wavelength (450/630 nm)

      High-precision transfer pettor, EP tubes and disposable pipette tips

      Incubator capable of maintaining 37℃

      Deionized or distilled water

      Absorbent paper

      Loading slot for Wash Buffer



      Performance

      Intra-CV:CV%<8%

      3 samples with low, mid-range and high level were tested 20 times on one plate respectively.

      Inter-CV:CV%<10%

      3 samples with low, mid-range and high level were tested on 3 different plates, 20 replicates in each plate.


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