SARS-CoV-2 Spike Protein Total Antibody ELISA Kit

    • sandwich-Ag-ELISA-Elabscience
    • sandwich-Ag-ELISA-Elabscience
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    • sandwich-Ag-ELISA-Elabscience
    • sandwich-Ag-ELISA-Elabscience
    • sandwich-Ag-ELISA-Elabscience
    • sandwich-Ag-ELISA-Elabscience

      Catalog number:E-EL-E607

      Synonyms:COVID 19 Spike Protein Total Antibody,Spike Protein Total Antibody,Spike Protein Antibody,SP Total Antibody,SP Antibody,SARS-CoV-2 Spike(RBD) Tab、2019-nCoV Spike(RBD) Tab、COVID-19 Spike(RBD) Tab

      Size:
      • 96T
      • 24T
      • 96T*5
      • 96T*10
      Qty:
      - +
      Price: $576

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Intended use

      This ELISA kit applies to the in vitro qualitative determination of total antibodies against SARS-CoV-2 Spike Protein in serum or plasma.

       

      Test principle

      This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.

       

      Kit components

      An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the conditions on the next page once the kit is received.

      Item

      Specifications

      Storage

      Micro ELISA Plate (Dismountable)

      96T: 8 wells ×12 strips
      24T: 8 wells ×3 strips
      96T*5: 5 plates, 96T

      -20℃, 6 months

      Positive Control

      96T: 2 vials
      24T: 1 vial
      96T*5: 10 vials

      Negative Control

      96T: 2 vials
      24T: 1 vial
      96T*5: 10 vials

      Concentrated HRP Conjugated Spike Protein (HRP-SP, 100×)

      96T: 1 vial, 120 μL
      24T: 1 vial, 60 μL
      96T*5: 5 vials, 120 μL

      -20℃(Protect from light), 6 months

      Sample & Control Diluent

      96T/24T: 1 vial, 20 mL
      96T*5: 5 vials, 20 mL

      2-8℃, 6 months

      HRP Conjugate Diluent

      96T/24T: 1 vial, 14 mL
      96T*5: 5 vials, 14 mL

      Concentrated Wash Buffer(25×)

      96T/24T: 1 vial, 30 mL
      96T*5: 5 vials, 30 mL

      Substrate Reagent

      96T/24T: 1 vial, 10 mL
      96T*5: 5 vials, 10 mL

      2-8℃(Protect from light)

      Stop Solution

      96T/24T: 1 vial, 10 mL
      96T*5: 5 vials, 10 mL

      2-8℃

      Plate Sealer

      96T/24T: 5 pieces
      96T*5: 25 pieces

       

      Product Description

      1 copy

      Certificate of Analysis

      1 copy

      Note:All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).


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