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SARS-CoV-2 Spike Protein Total Antibody ELISA Kit

  • Cat.No.:E-EL-E607

  • Assay Type: Sandwich-ELISA
  • Assay time: 2.5h

To Purchase E-EL-E607

Size:
  • 96T
  • 24T
  • 96T*5
  • 96T*10
Price: $576
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Properties

Format 24T, 96T, 96T*5, 96T*10
Assay time 2.5h
Sample type Serum or plasma
Sample volume 100 μL
Storage 2-8℃
Interpretation Qualitative
Application This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Spike Protein Total Antibody in serum or plasma.
Reproducibility Both intra-CV and inter-CV are < 10%
Synonyms COVID 19 Spike Protein Total Antibody,Spike Protein Total Antibody,Spike Protein Antibody,SP Total Antibody,SP Antibody,SARS-CoV-2 Spike(RBD) Tab、2019-nCoV Spike(RBD) Tab,COVID-19 Spike(RBD) Tab

Test Principle

This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.

Assay Procedures

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Citations

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