SM2(Sulfamethazine) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E043


      • 96T
      - +
      Price: $260

      sample_type: Muscle,Milk,Urine,Honey

      Sensitivity: 0.5 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Sulfamethazine (SM2) in samples, such as muscle, honey. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, SM2 in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- SM2 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of SM2. The concentration of SM2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

      Technical indicator

      Sensitivity: 0.5 ppb (ng/mL)

      Reaction mode: 25, 45 min~ 15 min

      Detection limit: Muscle (method 1) ---0.5 ppb, Milk ---10 ppb,

      Muscle (method 2) ---2.5 ppb, Urine---2 ppb, Honey---0.5 ppb.

      Cross-reactivity: Sulfamethazine ---100%.

      Sample recovery rate: Muscle, Honey---95%±25%, Urine, Milk ---85%±25%.

      Kits components



      ELISA Microtiter plate

      96 wells

      Standard Liquid

       1 mL each

      (0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb)

      HRP Conjugate

      5.5 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      2×Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece


      1 copy

      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      Micropipette: Single channel (20-200 μL, 100-1000 μL), Multichannel (30-300 μL).

      Reagents: Ethyl acetate, N-hexane, Acetonitrile, Na2HPO412H2O, NaOH, Concentrated HCl,  NaH2PO42H2O.

      Storage and expiry date

      Store the kit at 2~8. Do not freeze any test kit components.

      Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8.

      Expiry date: expiration date is on the packing box.

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      MSDS for ELISA ISO 9001

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