SM (Spectinomycin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    <
    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E081

      Size:
      • 96T
      Qty:
      - +
      Price: $276

      sample_type: Raw milk,Tissue

      Sensitivity: 0.2 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect spectinomycin (SM) in samples, such as tissues, raw milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, SM in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-SM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of SM. The concentration of SM in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.2 ppb (ng/mL)

      Reaction mode: 25℃,30 min~10 min

      Detection limit: Raw milk ---5 ppb; Tissue ---10 ppb

      Cross-reactivity: Spectinomycin---100%

         Streptomycin, Neomycin, Kanamycin, Gentamicin, Dihydrostreptomycin---0.01%

      Sample recovery rate: 90%±30%.



      Kits components

      Item

      Specifications

      ELISA Microtiter Plate

      96 wells

      Standard Liquid

      1.5 mL each

           (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb)

      20×Concentrated Pork Solution

      5 mL

      HRP Conjugate

      7 mL

      Antibody Working Solution

      7 mL

      Substrate Reagent A

      7 mL

      Substrate Reagent B

      7 mL

      Stop Solution

      7 mL

      60×Concentrated Sample Solution

      15 mL

      20×Concentrated Wash Buffer

      25 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy



      Other supplies required

      Instrument: Microplate reader, Oscillators, Centrifuge,  Balance (sensibility 0.01g),

      Micropipettor:  Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 



      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.




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