25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit (E-EL-0015)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 25-HVD3. During the reaction, Universal 25-HVD3 in the sample or standard competes with a fixed amount of Universal 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

25-hydroxyvitamin D, also known as 25-hydroxyvitamin D3, is the main form of vitamin D in the body. It is produced by cholecalciferol (vitamin D3) under the induction of 25-hydroxylase in the liver.The main functions of 25-hydroxyvitamin D are to promote the absorption of calcium and phosphorus by the small intestinal mucosa, promote osteoclast activity and enhance the mobilization of bone calcium by the parathyroid glands, and promote the reabsorption of calcium by the renal tubules, etc.