AA(Arachidonic Acid) ELISA Kit (E-EL-0051)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal AA. During the reaction, Universal AA in the sample or standard competes with a fixed amount of Universal AA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal AA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal AA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Arachidonic Acid, also known as arachidonic acid (AA or ARA), is an important polyunsaturated fatty acid. Its chemical name is pancis-5,8,11,14-eicosatetraenoic acid (CIS-5,8,11,14-eicosatetraenoic acid), the molecular formula is C20H32O2, the molecular weight is 304.46, and the CAS number is 506-32-1. Arachidonic acid is liquid at room temperature, with a melting point of -49.5 ° C and a boiling point of 245 ° C, and can be dissolved in alcohols, ethers and water. It is widely distributed in the neutral fat of animals, is one of the most abundant and widely distributed polyunsaturated fatty acids in mammals, and is also an important essential fatty acid in human body. Arachidonic acid can produce many metabolites such as prostaglandin, throboxane, lipoxygenin, leukotriene, hydroxy-eicosatetraenoic acid and epoxide eicosatetraenoic diol through three metabolic pathways regulated by cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP450). These metabolites are widely involved in organ function maintenance, inflammatory response, drug metabolism and other physiological processes, and play an important role in the occurrence and development of cardiovascular diseases, cancer and other diseases