Arginase Activity Colorimetric Assay Kit (E-BC-K848-M)
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For research use only.
| Detection Principle | Arginase is a key enzyme in the urea cycle. Arginase plays an important role in tumor, cardiovascular disease and so on. The principle of this kit is that arginase catalyzes substrate decomposition to produce urea, and urea reacts with chromogenic agent to produce chromogenic substance. The optimal detection wavelength of this product is 520 nm. By detecting the amount of chromogenic substance produced per unit time, the activity of arginase in the sample can be calculated. |
| Sample Type | Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-540 nm, optimum wavelength: 520 nm) |
| Research Area | Oxidative Stress , Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), Deionized water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.07 U/L |
| Detection Range | 0.07-23.40 U/L |
| Precision | inter-assay CV: 7.2-10.0% | intra-assay CV: 2.5-4.8% |
| Sample Volume | 50 μL(tissue/cell homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 20-120 |
| 1×10^6 293T cells | 1 |
| 1×10^6 HepG2 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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