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Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (E-BC-K236-S)

All Size Price Qty
100Assays $ 180.00
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For research use only.

Detection Principle AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Synonyms AST;GOT
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (505 nm)
Research Area Liver And Renal Function
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.38 IU/L
Detection Range 0.38-72.30 IU/L
Precision inter-assay CV: 4.9% | intra-assay CV: 4.2%
Assay Time 1 h 40 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human plasma 1
Human serum 1
10% Rat liver tissue homogenate 16-32
HepG2 supernatant 1
Rat serum 1
Mouse plasma 1
10% Rat kidney tissue homogenate 1
10% Rat heart tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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