cAMP (Cyclic adenosine monophosphate) ELISA Kit (E-EL-0184)

"This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. During the reaction, cAMP in samples or Standard competes with a fixed amount of cAMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cAMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve."

cAMP is a ubiquitous intracellular second messenger mediating diverse cellular processes from bacteria to higher eukaryotes. It is converted from ATP by adenylyl cyclases (ACs) and inactivated by phosphodiesterases (PDEs) via hydrolysis to 5'-AMP. In mammals, GPCRs linked to Gαs activate transmembrane ACs to increase cAMP, while those associated with Gαi/o inhibit cAMP synthesis. cAMP binds and activates PKA, Epac1/2, and cyclic nucleotide-gated ion channels, and can be released extracellularly and converted to adenosine. With a mass of ~329.21 g/mol, cAMP is synthesized from ATP by adenylate cyclase, notably in neurons and endocrine tissues. It acts by activating PKA to phosphorylate target proteins, regulating metabolism, gene expression, and cell growth. As a key GPCR signaling component, cAMP modulates ion channels and enzymes, participates in feedback loops for homeostasis, and integrates into cAMP-dependent and calcium signaling pathways. It works with CREB to regulate transcription and is degraded by PDEs for transient responses. Irregular cAMP levels associate with heart failure, certain cancers, and cystic fibrosis (via CFTR), highlighting its critical role in cellular function and signaling balance.