Cell Copper (Cu2+) Colorimetric Assay Kit (Complexing Method) (E-BC-K775-M)

For research use only.
Detection Principle | In acidic condition, the copper ion in the sample react with complexing agent to form a purple complex which has a maximum absorption peak at 580 nm. And copper ion content can be calculated indirectly by measuring the OD value at 580 nm. |
Sample Type | Cell |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (575-585 nm) |
Research Area | Inorganic Ions , Cuproptosis |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 0.18 μmol/L |
Detection Range | 0.18-5 μmol/L |
Precision | inter-assay CV: 3.1% | intra-assay CV: 0.03 |
Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
293T cell | 1 |
Molt-4 cell | 1 |
Jurkat cell | 1 |
Hela cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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