Cell Copper (Cu2+) Colorimetric Assay Kit (Complexing Method) (E-BC-K775-M)
For research use only.
| Detection Principle | In acidic condition, the copper ion in the sample react with complexing agent to form a purple complex which has a maximum absorption peak at 580 nm. And copper ion content can be calculated indirectly by measuring the OD value at 580 nm. |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (575-585 nm) |
| Research Area | Inorganic Ions , Cuproptosis |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.18 μmol/L |
| Detection Range | 0.18-5 μmol/L |
| Precision | inter-assay CV: 3.1% | intra-assay CV: 0.03 |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 293T cell | 1 |
| Molt-4 cell | 1 |
| Jurkat cell | 1 |
| Hela cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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