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Cell Copper (Cu2+) Colorimetric Assay Kit (Complexing Method) (E-BC-K775-M)

All Size Price Qty
96T $ 300.00
48T $ 200.00
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For research use only.

Detection Principle In acidic condition, the copper ion in the sample react with complexing agent to form a purple complex which has a maximum absorption peak at 580 nm. And copper ion content can be calculated indirectly by measuring the OD value at 580 nm.
Sample Type Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (575-585 nm)
Research Area Inorganic Ions ,  Cuproptosis
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.18 μmol/L
Detection Range 0.18-5 μmol/L
Precision inter-assay CV: 3.1% | intra-assay CV: 0.03
Assay Time 30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
293T cell 1
Molt-4 cell 1
Jurkat cell 1
Hela cell 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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