CellaQuant™ Human IFN-β (Interferon Beta) ELISA Kit (CQH017)

Name: CellaQuant™ Human IFN-β (Interferon Beta) ELISA Kit
Brand: Elabscience
SKU: CQH017
Price: 150.0 USD
Availability: InStock

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CellaQuant™ Human IFN-β (Interferon Beta) ELISA Kit - 1

Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

The CellaQuant^TM series of kits independently developed by Elabscience^® are specifically designed for the detection of cell samples, enabling accurate quantification of the expression levels of target proteins (such as cytokines) in cell supernatants and cell lysates. These products have been strictly optimized and thoroughly validated for typical cell samples, featuring high sensitivity, high specificity, and low background interference to ensure the accuracy and reliability of detection results. The CellaQuant^TM series is developed in strict accordance with clinical quality control standards. Some key parameters are traceable to to international standards, and they have excellent batch-to-batch consistency, providing a stable and reproducible analytical tool for protein expression research at the cellular level.

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IFN-β in samples.No significant cross-reactivity or interference between and analogues was observed
Recovery	80%-120%
Sample Type	Cell culture supernatant, Cell lysates or other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 17 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.

Background

Interferon-beta (IFN-β), a type I interferon, plays a key role in innate immunity against infections, tumors and inflammatory stimuli. Produced by fibroblasts, dendritic cells, macrophages and endothelial cells, it exerts diverse functions via specific signaling pathways and is clinically used to treat multiple sclerosis (MS). IFN-β mainly signals by binding to the heterodimeric receptor (high-affinity IFNAR2 and low-affinity IFNAR1), activating the Jak-STAT pathway. This regulates interferon-responsive genes encoding antiviral, proliferation-regulating and immunoregulatory proteins. It can also function via IFNAR1 alone, independent of Jak-STAT, enhancing its signaling versatility.Its biological effects include antiviral/antibacterial activities, regulation of B-cell development, myelopoiesis and LPS-inducible tumor necrosis factor. Notably, it is more potent than IFN-α in inducing apoptotic and antiproliferative pathways for tumor control, and protects dopaminergic neurons by maintaining neuronal homeostasis.