CellaQuant™ Human IL-12 (Interleukin 12) ELISA Kit (CQH019)

Name: CellaQuant™ Human IL-12 (Interleukin 12) ELISA Kit
Brand: Elabscience
SKU: CQH019
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

CellaQuant™ Human IL-12 (Interleukin 12) ELISA Kit - 1

Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

The CellaQuant^TM series of kits independently developed by Elabscience^® are specifically designed for the detection of cell samples, enabling accurate quantification of the expression levels of target proteins (such as cytokines) in cell supernatants and cell lysates. These products have been strictly optimized and thoroughly validated for typical cell samples, featuring high sensitivity, high specificity, and low background interference to ensure the accuracy and reliability of detection results. The CellaQuant^TM series is developed in strict accordance with clinical quality control standards. Some key parameters are traceable to to international standards, and they have excellent batch-to-batch consistency, providing a stable and reproducible analytical tool for protein expression research at the cellular level.

Sensitivity	0.38 pg/mL
Detection Range	0.63-40 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IL-12/P70 in samples.No significant cross-reactivity or interference between and analogues was observed
Recovery	80%-120%
Sample Type	Cell culture supernatant, Cell lysates or other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 19 nm. The OD value is proportional to the concentration of Human IL-12. You can calculate the concentration of Human IL-12 in the samples by comparing the OD of the samples to the standard curve.

Background

IL-12 p70 is the biologically active heterodimer of interleukin-12 (IL-12), consisting of disulfide-linked IL-12A (p35) and IL-12B (p40) subunits with a molecular weight of approximately 70 kDa. It is primarily produced by professional antigen-presenting cells (APCs) following antigen stimulation, which distinguishes it from inactive p40 monomers or homodimers. Functionally, IL-12 p70 induces interferon-gamma (IFN-γ) production, promotes the differentiation of naive T cells into T-helper 1 (Th1) cells, and enhances the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). It exerts its effects by binding to the IL12Rβ1/β2 heterodimeric receptor, which activates JAK-STAT4 signaling to regulate cytokine-responsive gene expression.IL-12 p70 shares the p40 subunit with IL-23 and the p35 subunit with IL-35. As a key link between innate and adaptive immunity, it plays a vital role in research on infectious diseases, autoimmune disorders, and cancer immunotherapy.